| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Dictyostelium amoebas expressing GFP myosin II were dispersed on substrates of different stiffness. Cell migration and myosin II dynamics of them were measured in detail. On a soft substrate, the cells produced few filopodia-like pseudopodia, while on a stiff substrate, the cells produced many filopodia-like pseudopodia. A detailed analysis of pseudopodia behavior revealed that the maximum pseudopodia length was shorter for cells on a hard substrate. The maximum fluorescence intensity of myosin II was stronger in cells on a stiff substrate. These results suggest that the accumulation of myosin II and pseudopodia-dynamics depends on the degree of cell cortex extension.
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