Molecular Mechanisms of Regulation of P680 Redox Potential in Photosystem II
| Project/Area Number |
17K07367
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Ehime University |
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Principal Investigator |
Sugiura Miwa 愛媛大学, プロテオサイエンスセンター, 准教授 (80312255)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 光合成電子伝達 / 光化学系II / P680 / 電荷分離 / 光励起 / クロロフィル / 光阻害 / 光合成電子伝達系 / 三重項 / 光合成 / 電子伝達 |
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| Outline of Final Research Achievements |
The chlorophyll, ChlD1, which is located between the PD1PD2 chlorophyll pair and the PheoD1 is the longest wavelength chlorophyll in the heart of Photosystem II. Its central Mg2+ is liganded to a water molecule that is H-bonded to D1/T179. In this study, site-directed mutants were made in the thermophilic cyanobacterium, and characterized by a range of biophysical techniques. TL indicate that i) radiative recombination occurs via the repopulation of *ChlD1 itself; ii) non-radiative charge recombination reactions appeared to be faster in the T179H-PSII; and iii) the properties of PD1PD2 were unaffected by this mutation, and consequently iv) the immediate precursor state of the radiative excited state is the ChlD1+PheoD1－ radical pair. Chlorophyll bleaching due to high light correlated with the amount of 1O2 generated. The ChlD1 itself is the chlorophyll that is first damaged by 1O2. Thus, ChlD1 appears to be one of the primary damage site in recombination-mediated photoinhibition.
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| Academic Significance and Societal Importance of the Research Achievements |
シアノバクテリアや植物が行う光合成は地球上の生命を維持する非常に重要な反応である。そして好気的生物の生存を支えるほど高い効率で反応を行う光合成の分子反応機構を理解することは、サステイナブルエネルギー開発の強力な参考になるため、解明が急務である。しかし、光合成の反応の特に重要で基本的な部分については未だ不明な点が多い。本研究では、光合成の初発の反応である、太陽光によって光合成タンパク質に結合したクロロフィル分子の励起、電荷分離という、太陽光発電に通じる部分の分子機構について明らかにした。本研究成果は学術的に重要であるだけでなく、次世代エネルギー開発の基盤となることが期待でき、社会的意義は大きい。
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Probing the role of Arginine 323 of the D1 protein in Photosystem II function2020
Author(s)
Sugiura, M., Taniguchi, T., Tango, N., Nakamura, M., Selles, J., and Boussac, A.
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Journal Title
Physiol. Plant.
Volume: -
Issue: 2
Pages: 183-199
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] New insights on ChlD1 function in Photosystem II from site-directed mutants of D1-T179 in Thermosynechococcus elongatus2019
Author(s)
Takegawa, Y., Nakamura, M., Nakamura, S., Noguchi, T., Selles, J., Rutherford, A. W., Boussac, A., and Sugiura, M.
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Journal Title
Biochim. Biophys. Acta (Bioenergetics)
Volume: 1860
Issue: 4
Pages: 297-309
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Probing the role of Valine 185 of the D1 protein in the Photosystem II oxygen evolution2018
Author(s)
Sugiura, M., Tibiletti, T., Takachi, I., Hara, Y., Kanawaku, S., Selles, J., and Boussac, A
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Journal Title
Biochim. Biophys. Acta (Bioenergetics)
Volume: 1859
Issue: 12
Pages: 1259-1273
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] An ATP generation system for in vitro motility assay2017
Author(s)
Kabir, A. M. R., Ito, M., Uenishi. K., Anan, S., Konagaya, A., Sada. K., Sugiura, M., and Kakugo, A.
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Journal Title
Chem. Lett
Volume: 46
Pages: 178-180
Related Report
Peer Reviewed / Int'l Joint Research
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