Search for important sites in protein structure formation and elucidation of selection factors in their evolution
Project/Area Number |
17K07368
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biophysics
|
Research Institution | Kyoto Prefectural University |
Principal Investigator |
Takano Kazufumi 京都府立大学, 生命環境科学研究科, 教授 (40346185)
|
Project Period (FY) |
2017-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | タンパク質 / 立体構造 / アミノ酸変異 / 配列アライメント / フォールディング / 進化 |
Outline of Final Research Achievements |
In this work, in order to get broader knowledge on the relationship between amino acid substitutions and protein function/structure, we attempted to extract ‘mutable and fatal sites’ by ‘random mutation’ using ‘stable hyperthermophilic proteins’. The proteins applied were an esterase from Sulfolobus tokodaii (Sto-Est) and a subtilisin from Thermococcus kodakarensis (Tk-Sub). These proteins have been well studied for their function, structure and stability, and have also been used in random mutation experiments; we have extensive published and unpublished data on the activity and sequence for random mutations of these proteins. This study has revealed a portion of the properties at conserved sites that can be mutated and non-conserved sites that are not suitable for mutation.
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Academic Significance and Societal Importance of the Research Achievements |
今日、タンパク質の構造構築メカニズムに関して、計算機を用いた研究が増加してきているが、実験を通して得られる知見もまだまだ多くある。特に本研究のような網羅的解析が有効である。本研究は、研究代表者がこれまで行ってきた個々のタンパク質の安定性・フォールディング研究から構造構築網羅的解析、物性と進化の関係も含んだ新たな研究を展開するものである。
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Report
(4 results)
Research Products
(16 results)