| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In this work, in order to get broader knowledge on the relationship between amino acid substitutions and protein function/structure, we attempted to extract ‘mutable and fatal sites’ by ‘random mutation’ using ‘stable hyperthermophilic proteins’. The proteins applied were an esterase from Sulfolobus tokodaii (Sto-Est) and a subtilisin from Thermococcus kodakarensis (Tk-Sub). These proteins have been well studied for their function, structure and stability, and have also been used in random mutation experiments; we have extensive published and unpublished data on the activity and sequence for random mutations of these proteins. This study has revealed a portion of the properties at conserved sites that can be mutated and non-conserved sites that are not suitable for mutation.
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