Quantification of oocyte aging by analyzing mitochondrial thermogenesis
| Project/Area Number |
17K07372
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Itabashi Takeshi 国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 (20434384)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 細胞分裂 |
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| Outline of Final Research Achievements |
The heat acting inside and outside the cell is involved in the accuracy of cell division. Mitochondrion produces the heat within the cell and is an organelle involved in oocyte aging. In this study, we attempted to clarify the relationship between heat production of meiotic mitochondria and, characteristics and quality of oocyte. It was suggested that mitochondrial temperature was increased during meiosis using jellyfish oocytes. We also developed an experimental system for analyzing the amount, distribution, and ultrastructure of mitochondria in the identical oocyte by a three-dimensional electron microscope after measuring the thermodynamics with an optical microscope.
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| Academic Significance and Societal Importance of the Research Achievements |
昨今、老化した卵子にミトコンドリア移植を行うという治療法が、1つの有効な不妊治療として注目されている。つまり、老化した卵子のミトコンドリアの性質を多面的に精査することが急務である。本研究は、光学顕微鏡による細胞温度計測と先端電子顕微鏡による微細構造解析という新たな観点から、減数分裂の失敗に起因する先天性疾患や不妊症を引き起こす卵細胞の特性・品質の解明と、その診断法開発に向けて新規な基盤を与えることが期待される。
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Report
(4 results)
Research Products
(6 results)
