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Quantification of oocyte aging by analyzing mitochondrial thermogenesis

Research Project

Project/Area Number 17K07372
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Itabashi Takeshi  国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 (20434384)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords細胞分裂
Outline of Final Research Achievements

The heat acting inside and outside the cell is involved in the accuracy of cell division. Mitochondrion produces the heat within the cell and is an organelle involved in oocyte aging. In this study, we attempted to clarify the relationship between heat production of meiotic mitochondria and, characteristics and quality of oocyte. It was suggested that mitochondrial temperature was increased during meiosis using jellyfish oocytes. We also developed an experimental system for analyzing the amount, distribution, and ultrastructure of mitochondria in the identical oocyte by a three-dimensional electron microscope after measuring the thermodynamics with an optical microscope.

Academic Significance and Societal Importance of the Research Achievements

昨今、老化した卵子にミトコンドリア移植を行うという治療法が、1つの有効な不妊治療として注目されている。つまり、老化した卵子のミトコンドリアの性質を多面的に精査することが急務である。本研究は、光学顕微鏡による細胞温度計測と先端電子顕微鏡による微細構造解析という新たな観点から、減数分裂の失敗に起因する先天性疾患や不妊症を引き起こす卵細胞の特性・品質の解明と、その診断法開発に向けて新規な基盤を与えることが期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (6 results)

All 2020 2019 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.2020

    • Author(s)
      Miyamoto T, Hosoba K, Itabashi T, Iwane AH, Akutsu AN, Ochiai H, Saito Y, Yamamoto T, Matsuura S
    • Journal Title

      EMBO Journal

      Volume: - Issue: 12

    • DOI

      10.15252/embj.2019103499

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 細胞伸展刺激に起因する細胞核動態変化の微細構造解析2019

    • Author(s)
      板橋 岳志, 一ノ瀨 孝子, 島田 文子, 森 ひかり, 平原 麻利子, 山本 華代, 岩根 敦子
    • Organizer
      日本顕微鏡学会 第75回学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 発生過程を先端顕微鏡解析から得られた3D構造モデルから読み解く2019

    • Author(s)
      板橋 岳志, 一ノ瀨 孝子, 黒田 純平, 近藤 滋, 岩根 敦子
    • Organizer
      日本顕微鏡学会 第75回学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 細胞丸ごと 超微細構造イメージング: 細胞を三次元電子顕微鏡構造モデルから読み解く2019

    • Author(s)
      板橋 岳志
    • Organizer
      「細胞を創る」研究会 12.0
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Examining force regulation of anaphase cell2018

    • Author(s)
      Takeshi Itabashi, Shin’ichi Ishiwata
    • Organizer
      第56回生物物理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Responses of the mitotic spindle to mechanical perturbations2018

    • Author(s)
      板橋岳志
    • Organizer
      THE 3RD HIROSHIMA INTERNATIONAL SYMPOSIUM ON FUTURE SCIENCE “FRONTIERS IN BIOIMAGING BASED LIFE SCIENCE”
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2017-04-28   Modified: 2021-02-19  

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