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Structural dynamics and cooperativity of axonemal dyneins

Research Project

Project/Area Number 17K07376
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics
Research InstitutionNational Institute of Information and Communications Technology

Principal Investigator

Oiwa Kazuhiro  国立研究開発法人情報通信研究機構, 未来ICT研究所, 主管研究員 (10211096)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords鞭毛 / 軸糸 / ダイニン / タンパク質モータ / X線繊維回折 / 原子間力顕微鏡 / 軸糸ダイニン / 分子モーター / 鞭毛運動
Outline of Final Research Achievements

To understand the coordination mechanism of dynein arms in the eukaryotic flagellar axoneme, we have investigated the structural dynamics of axonemal dyneins in the axoneme using the atomic force microscopy (AFM) and the X-ray fiber diffraction (XFD) under the physiological conditions. AFM confirmed the 24nm structural repeat of outer dynein arms in the topology of the axonemes. AFM imaging thus defined a detailed map of axonemal dyneins under the aqueous environment and provided the dynamics of these components.
In XFD studies, the axonemes aligned in the shear-flow were irradiated with the intense and stable X-ray in SPring-8, BL40XU. We explored the spatial arrangement of axonemal components under different [Ca2+], suggesting the change in the helical symmetry of 9 doublets in the axoneme coupled with [Ca2+]. Since Chlamydomonas changes the ciliary waveform to the flagellar one in response to the change in [Ca2+], the helical symmetric would relate to the waveform generation.

Academic Significance and Societal Importance of the Research Achievements

鞭毛・繊毛運動の理解は、その生理学的重要性と、繊毛の機能不全が引き起こす疾病群があることから、医学的重要性が高い。鞭毛運動の解析は前述の疾病発症メカニズムの解明につながり、さらには診断法、治療法の開発につながることが期待される。
これに加えて、周囲の溶媒から激しい熱運動を受けながらも、高いエネルギー変換効率で一方向の運動を起こすダイニンの運動機能やアンサンブルとして機能する場合に示す高い協働性の知見は、分子マシン構築において重要である。自然が創り出した機能モジュールを組み合わせて構築する分子マシンの研究の進展において、軸糸の運動メカニズムの知見は、新たな設計指針を与えるものである。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (23 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (12 results) (of which Int'l Joint Research: 7 results,  Invited: 2 results) Book (2 results) Remarks (4 results)

  • [Int'l Joint Research] Max Planck Institute(ドイツ)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] Max Planck Institute(ドイツ)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Force-Generating Mechanism of Axonemal Dynein in Solo and Ensemble2020

    • Author(s)
      Kenta Ishibashi, Hitoshi Sakakibara, Kazuhiro Oiwa
    • Journal Title

      International Journal of Molecular Sciences

      Volume: - Issue: 8 Pages: 2843-2843

    • DOI

      10.3390/ijms21082843

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transport of microtubules according to the number and spacing of kinesin motors on gold nano-pillars2019

    • Author(s)
      Kaneko Taikopaul、Ando Suguru、Furuta Ken’ya、Oiwa Kazuhiro、Shintaku Hirofumi、Kotera Hidetoshi、Yokokawa Ryuji
    • Journal Title

      Nanoscale

      Volume: 000 Issue: 20 Pages: 1-9

    • DOI

      10.1039/c9nr01324e

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Flagellar-associated Protein FAP85 Is a Microtubule Inner Protein That Stabilizes Microtubules2018

    • Author(s)
      Junya Kirima and Kazuhiro Oiwa
    • Journal Title

      Cell Structure and Function

      Volume: 43 Issue: 1 Pages: 1-14

    • DOI

      10.1247/csf.17023

    • NAID

      130006356079

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Change in the helical symmetry of Chlamydomonas and Ciona flagellar axonemes coupled with the change in Ca2+ concentrations revealed by X-ray fiber diffraction2020

    • Author(s)
      Kazuhiro Oiwa, Kogiku Shiba, Kazuo Inaba, Hiroyuki Iwamoto, Hitoshi Sakakibara
    • Organizer
      64th Annual Meeting of the Biophysical Society
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] High-speed atomic force microscopy on outer dynein arms aligned on microtubules2019

    • Author(s)
      Kenta Ishibashi and Kazuhiro Oiwa
    • Organizer
      第57回 日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Structural changes of Chlamydomonas and Ciona flagellar axonemes coupled with the change in [Ca 2+] studied with X-ray fiber diffraction2019

    • Author(s)
      Kazuhiro Oiwa, Kogiku Shiba, Kazuo Inaba, Hiroyuki Iwamoto, Hitoshi Sakakibara
    • Organizer
      第57回 日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Changes in the helical symmetry of the axoneme of Chlamydomonas flagella coupled with Ca2+ concentrations2019

    • Author(s)
      Kazuhiro Oiwa, Hiroyuki Iwamoto, and Hitoshi Sakakibara
    • Organizer
      The American Society for Cell Biology/European Molecular Biology Organization 2019 Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Re-Design of Linear Molecular Motors2019

    • Author(s)
      Ryota Ibusuki, Akane Furuta, Kazuhiro Oiwa, Hiroaki Kojima, Ken'ya Furuta
    • Organizer
      Biophysical Society Annual Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Tubulin polymerization-promoting protein family member 3 (Tppp3) facilitates microtubule bundling and network formation via its weak interaction with microtubules2019

    • Author(s)
      Takayuki Torisawa, Shuji Ishihara, Kazuhiro Oiwa
    • Organizer
      Biophysical Society Annual Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Ca2+-dependent changes in helical symmetry of axonemal components of Chlamydomonas flagella studied by X-ray fiber diffraction2018

    • Author(s)
      Kazuhiro Oiwa, Hiroyuki Iwamoto
    • Organizer
      日本生物物理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] High-speed atomic force microscopic observations on demembranated Chlamydomonas axonemes and dynein arms2018

    • Author(s)
      Kenta Ishibashi, Kazuhiro Oiwa
    • Organizer
      日本生物物理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Repetitive buckling of microtubules driven by axonemal dynein arrays reconstituted on a microtubule2018

    • Author(s)
      Misaki Sagawa, Misaki Shiraga, Hitoshi Sakakibara, Kazuhiro Oiwa
    • Organizer
      日本生物物理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Creation of protein-based molecular motors moving on DNA nanostructure2018

    • Author(s)
      Kazuhiro Oiwa, Ryota Ibusuki, Ken'ya Furuta
    • Organizer
      THERMEC 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] High-speed atomic force microscopic observations on demembranated Chlamydomonas axonemes and dynein arms2018

    • Author(s)
      Misaki Shiraga, Yuka Matsuda, Junya Kirima, and Kazuhiro Oiwa
    • Organizer
      Biophysical Society Annual Meeting
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Helical arrangement of axonemal components is a key for determination and Ca2+-dependent switching of waveforms of Chlamydomonas flagella2017

    • Author(s)
      Kazuhiro Oiwa
    • Organizer
      International Workshop Dynein 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] Handbook of Dynein (Second Edition) Chapter 92019

    • Author(s)
      Misaki Shiraga, Junya Kirima, Naoki, Kanatani, Yousuke Shimizu, Hitoshi Sakakibara, Kazuhiro Oiwa
    • Total Pages
      436
    • Publisher
      Pan Stanford Publishing, Ed. Keiko Hirose
    • ISBN
      9789814800013
    • Related Report
      2018 Research-status Report
  • [Book] Dyneins: Dynein Mechanics, Dysfunction, and Disease, Chapter 1. Electron microscopy of isolated dynein complexes and the power stroke mechanism2017

    • Author(s)
      ED. S.M. King, Vol 2 Chapter 1. Kazuhiro Oiwa, Hitoshi Sakakibara, Ken'ya Furuta
    • Total Pages
      496
    • Publisher
      Academic Press
    • ISBN
      9780128094709
    • Related Report
      2017 Research-status Report
  • [Remarks] Google Scholar, Kazuhiro Oiwa

    • URL

      https://scholar.google.com/citations?user=LT6CPrAAAAAJ&hl=en

    • Related Report
      2018 Research-status Report
  • [Remarks] 情報通信研究機構 未来ICT研究所 主管研究員

    • URL

      http://www2.nict.go.jp/advanced_ict/oiwa/index.html

    • Related Report
      2018 Research-status Report
  • [Remarks] Google Scholar, Kazuhiro Oiwa

    • URL

      https://scholar.google.com/citations?hl=en&user=LT6CPrAAAAAJ&view_op=list_works&sortby=pubdate

    • Related Report
      2017 Research-status Report
  • [Remarks] 情報通信研究機構 未来ICT研究所 生体物性プロジェクト

    • URL

      http://www2.nict.go.jp/frontier/seitai/

    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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