Molecular mechanisms for de novo membrane formation.

• Project/Area Number: 17K07377
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: University of Tsukuba
• Principal Investigator: Suda Yasuyuki 筑波大学, 医学医療系, 助教 (10553844)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 生体膜 / 膜輸送 / 出芽酵母 / 胞子形成 / 細胞生物学

Outline of Final Research Achievements

In this study, one of the effector complesx of Rab GTPase sec4, Exocyst complex, was focused to analyze for the initiation of prospore membrane formation during sporulation in Saccharomyces cerevisiae. We have constructed auxin-inducible degron mutants for each subunit of Exocyst complex and analyzed for prospore membrane formation. Although each of them did not show complete sporulation defective phenotypes, each mutants showed reduced sporulation efficiency, suggesting the Exocyst complex functions in prospore membrane formation.

Academic Significance and Societal Importance of the Research Achievements

本研究で明らかにする膜構造形成の分子機構は、出芽酵母の前胞子膜のみならず様々なオルガネラにおいて、さらには種を超えて存在する。よって本研究により得られる知見を足がかりとして細胞内膜交通の基本的な分子基盤が明らかになることが期待される。本研究ではExocyst複合体の前胞子膜形成への寄与を明らかにした。他生物におけるポストゴルジ小胞同士の融合による新規膜構造形成としては精子頭部の先体や植物細胞の細胞分裂時の細胞盤形成が挙げられ、Exocyst複合体はこれら膜構造形成にも同様に機能することが考えられる。

Research Products

• [Journal Article] Pop2 Phosphorylation at S39 Contributes to the Glucose Repression of Stress Response Genes, HSP12 and HSP26 (2019)
  • Author(s): Lien PTK, Viet NTM, Mizuno T, Suda Y, Irie K.
  • Journal Title: PLoS One Volume: 14 Issue: 4 Pages: e0215064-e0215064
  • DOI: 10.1371/journal.pone.0215064
  • NAID: 120007133492
  • Peer Reviewed / Open Access
• [Journal Article] Visualization of secretory cargo transport within the Golgi apparatus (2019)
  • Author(s): Kurokawa K, Osakada H, Kojidani T, Waga M, Suda Y, Asakawa H, Haraguchi T, Nakano A.
  • Journal Title: J. Cell Biol. Volume: - Issue: 5 Pages: 1602-1618
  • DOI: 10.1083/jcb.201807194
  • NAID: 120007133506
  • Peer Reviewed / Open Access
• [Journal Article] Spatiotemporal dissection of the trans-Golgi network in budding yeast (2019)
  • Author(s): Takuro Tojima, Yasuyuki Suda, Midori Ishii, Kazuo Kurokawa, Akihiko Nakano
  • Journal Title: Journal of Cell Science Volume: 132 Issue: 15 Pages: 231159-231159
  • DOI: 10.1242/jcs.231159
  • Peer Reviewed / Open Access
• [Journal Article] Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network (2019)
  • Author(s): Zhao Shen-Bao、Suda Yasuyuki、Nakanishi Hideki、Wang Ning、Yoko-o Takehiko、Gao Xiao-Dong、Fujita Morihisa
  • Journal Title: Biochimica et Biophysica Acta (BBA) - General Subjects Volume: 1863 Issue: 6 Pages: 1147-1157
  • DOI: 10.1016/j.bbagen.2019.04.009
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Regulation of LRG1 expression by RNA‐binding protein Puf5 in the budding yeast cell wall integrity pathway (2018)
  • Author(s): Viet Nguyen Thi Minh、Duy Duong Long、Saito Kazuhiro、Irie Kaoru、Suda Yasuyuki、Mizuno Tomoaki、Irie Kenji
  • Journal Title: Genes to Cells Volume: 23 Issue: 12 Pages: 988-997
  • DOI: 10.1111/gtc.12646
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of ER-Golgi Transport Dynamics by GTPases in Budding Yeast (2018)
  • Author(s): Suda Yasuyuki、Kurokawa Kazuo、Nakano Akihiko
  • Journal Title: Front. Cell Dev. Biol. Volume: 5 Pages: 122-122
  • DOI: 10.3389/fcell.2017.00122
  • NAID: 120007128323
  • Peer Reviewed / Open Access
• [Journal Article] Activation of Rab GTPase Sec4 by its GEF Sec2 is required for prospore membrane formation during sporulation in yeast Saccharomyces cerevisiae (2017)
  • Author(s): Suda Yasuyuki、Tachikawa Hiroyuki、Inoue Ichiro、Kurita Tomokazu、Saito Chieko、Kurokawa Kazuo、Nakano Akihiko、Irie Kenji
  • Journal Title: FEMS Yeast Res. Volume: 18 Issue: 1 Pages: 1-1
  • DOI: 10.1093/femsyr/fox095
  • Peer Reviewed
• [Journal Article] Dynamic localization of a yeast development-specific PP1 complex during prospore membrane formation is dependent on multiple localization signals and complex formation. (2017)
  • Author(s): Tsuyoshi S Nakamura, Yumi Numajiri, Yuuya Okumura, Junji Hidaka, Takayuki Tanaka, Ichiro Inoue, Yasuyuki Suda, Tetsuo Takahashi, Hideki Nakanishi, Xiao-Dong Gao, Aaron M Neiman, and Hiroyuki Tachikawa
  • Journal Title: Mol. Biol. Cell Volume: 28 Issue: 26 Pages: 3881-3895
  • DOI: 10.1091/mbc.e17-08-0521
  • NAID: 120007134596
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Visualization of the membrane traffic in budding yeast. (2019)
  • Author(s): Yasuyuki Suda
  • Organizer: International graduate symposium on industrial biotechnology.
  • Int'l Joint Research / Invited
• [Presentation] 出芽酵母減数分裂における膜交通関連オルガネラの再構成. (2019)
  • Author(s): 須田恭之，舘川宏之，中野明彦, 入江賢児
  • Organizer: 酵母遺伝学フォーラム第52回研究報告会
• [Presentation] 出芽酵母減数分裂における膜交通関連オルガネラの再構成. (2019)
  • Author(s): 須田恭之，舘川宏之，中野明彦, 入江賢児
  • Organizer: 第19回日本蛋白質科学会年会・第71回日本細胞生物学会大会合同年次大会
• [Presentation] Live imaging of the Golgi apparatus in Saccharomyces cerevisiae (2018)
  • Author(s): Yasuyuki Suda
  • Organizer: Mini-symposium on membrane trafficking and remodeling
  • Int'l Joint Research / Invited
• [Presentation] 出芽酵母前胞子膜伸長における PI4P およびオルガネラ接触部位の 役割の解析 (2018)
  • Author(s): 中村 毅, 棟重賢治, 須田恭之, 舘川宏之
  • Organizer: 酵母遺伝フォーラム第51回研究報告会
• [Presentation] Post-Golgi vesicle tethering and fusion for de novo membrane formation during sporulation in budding yeast (2018)
  • Author(s): Yasuyuki Suda, Hiroyuki Tachikawa, Kenji Irie, Akihiko Nakano
  • Organizer: The 2018 FEBS Golgi meeting
  • Int'l Joint Research
• [Presentation] Cytoplasmic Deadenylase Ccr4 is Required for Translational Repression of Puf5 mRNA targets in the Stationary Phase in Saccharomyces cerevisiae (2018)
  • Author(s): Long-Duy Duong, Yasuyuki Suda, Kenji Irie
  • Organizer: 2018 joint annual meeting of JSCB & JSDB
• [Presentation] Analysis of the role of PI4P and organelle contact site in prospore membrane extension during sporulation of budding yeast (2018)
  • Author(s): Tsuyoshi S. Nakamura, Kenji Muneshige, Yasuyuki Suda, Hiroyuki Tachikawa
  • Organizer: 2018 joint annual meeting of JSCB & JSDB
• [Presentation] Cytoplasmic deadenylases Ccr4 regulates not only LRG1 mRNA level through po;y(A) shortening but also the translation of LRG1 mRNA in the stationary phase. (2017)
  • Author(s): Kenji Irie, Duong Long Duy, Yasuyuki Suda
  • Organizer: Cutting edge development in RNA biology for the control of gene expression
  • Int'l Joint Research
• [Presentation] Cytoplasmic deadenylases Ccr4 is required for translational repression of Puf5 mRNA targets in the stationary phase. (2017)
  • Author(s): Duong Long Duy, Yasuyuki Suda, Kenji Irie
  • Organizer: Cutting edge development in RNA biology for the control of gene expression
  • Int'l Joint Research
• [Presentation] ポリA分解酵素Ccr4はLRG1 mRNAのポリ(A)鎖長と翻訳を制御する。 (2017)
  • Author(s): Duong Long Duy, 須田恭之, 入江賢児
  • Organizer: 酵母遺伝学フォーラム第50回研究報告会
• [Presentation] Cytoplasmic deadenylases Ccr4 and Pop2 are required for translational repression of LRG1 mRNA in the stationary phase. (2017)
  • Author(s): Arvin Lapiz Valderrama, Duong Long Duy, Yasuyuki Suda, Kenji Irie
  • Organizer: 28th International Conference on Yeast Genetics and Molecular Biology
  • Int'l Joint Research
• [Presentation] Membrane traffic in Saccharomyces cerevisiae (2017)
  • Author(s): Yasuyuki Suda
  • Organizer: Special seminar in College of Life Science, National Taiwan University
  • Int'l Joint Research / Invited
• [Presentation] Cytoplasmic deadenylases Ccr4 is required for translational repression of LRG1 mRNA in the stationary phase. (2017)
  • Author(s): Duong Long Duy, Yasuyuki Suda, Kenji Irie
  • Organizer: 第19回日本RNA学会年会
• [Presentation] 出芽酵母Sec2の胞子形成における役割 (2017)
  • Author(s): 須田恭之, 舘川宏之, 中野明彦, 入江賢児
  • Organizer: 第69回日本細胞生物学会大会
• [Presentation] 出芽酵母の前胞子膜伸張に必須なPP1ターゲティングサブユニットGip1の解析 (2017)
  • Author(s): 中村毅, 奥村裕哉, 井上一朗, 須田恭之, 舘川宏之
  • Organizer: 第69回日本細胞生物学会大会
• [Remarks] トランスゴルジ網の時空間ダイナミクス
  • URL: https://www.riken.jp/press/2019/20190711_2/
• [Remarks] タンパク質がゴルジ体内を輸送される仕組みが明らかに
  • URL: https://www.riken.jp/press/2019/20190311_4/