| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In this study, we focused on vps45 gene, which is one of the causative genes identified in severe congenital neutropenia (SCN). As an model animal, we used the nematode Caenorhabditis elegans (C. elegans) and conducted molecular genetic and molecular physiological studies of vps45. (1) To identify novel genes involved in vps45, we conducted genetic screenings for suppressor mutations of a vps45 mutant and obtained multiple suppressor mutant alleles. (2) We have developed a transgenic animal habouring human vps45 mutations in severe congenital neutropenia. (3) We developed a calcium imaging system to analyze calcium dynamics in apoptotic cells and neural activity in the SCN disease model.
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