Analysis of physiological function of pc-ECV
| Project/Area Number |
17K07381
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Hiroshima University (2018-2019)
Hamamatsu University School of Medicine (2017) |
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Principal Investigator |
IKEGAMI KOJI 広島大学, 医系科学研究科(医), 教授 (20399687)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 一次繊毛 / 細胞外小胞 / エクソソーム / 線毛 / 繊毛 |
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| Outline of Final Research Achievements |
We established a method to specifically concentrate and purify primary cilia-derived extracellular vesicles and established a cell line that does not specifically release primary cilia-derived extracellular vesicles, and succeeded in establishing an experimental system that can verify the physiological functions of primary cilia-derived extracellular vesicles. By making full use of these methods, we succeeded in finding that primary cilia-derived vesicles were actually taken up by the target cells and that the cell migration of the target cells was enhanced as a result. These methodologies and findings have been reported in international scientific journals, Japanese language science journals, and various academic conferences.
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| Academic Significance and Societal Importance of the Research Achievements |
新しい細胞間コミュニケーションツールとして機能する一次繊毛由来細胞外小胞の一側面が明らかになったことは，基礎生命科学の中でも細胞生物学に留まらず古典的な内分泌学にも新しい影響を与えうると思われ学術的意義が期待できる．また，一次繊毛由来細胞外小胞を濃縮精製する方法の確立，一次繊毛由来細胞外小胞の細胞内への取り込みの実証は，その医学・薬学など広い分野における産業利用に向けた基盤になる可能性もあり社会的意義が期待できる．
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Copy number variation in DRC1 is the major cause of primary ciliary dyskinesia in the Japanese population.2020
Author(s)
Takeuchi K, Xu Y, Kitano M, Chiyonobu K, Abo M, Ikegami K, Ogawa S, Ikejiri M, Kondo M, Gotoh S, Nagao M, Fujisawa T, Nakatani K.
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Journal Title
Mol Genet Genomic Med
Volume: 8(3)
Issue: 3
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] UBL3 modification influences protein sorting to small extracellular vesicles.2018
Author(s)
Ageta H, Ageta-Ishihara N, Hitachi K, Karayel O, Onouchi T, Yamaguchi H, Kahyo T, Hatanaka K, Ikegami K, Yoshioka Y, Nakamura K, Kosaka N, Nakatani M, Uezumi A, Ide T, Tsutsumi Y, Sugimura H, Kinoshita M, Ochiya T, Mann M, Setou M, Tsuchida K.
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Journal Title
Nature Communications
Volume: 9
Issue: 1
Pages: 3936-3936
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Environmental responsiveness of tubulin glutamylation in sensory cilia is regulated by the p38 MAPK pathway2018
Author(s)
Kimura Y, Tsutsumi K, Konno A, Ikegami K, Hameed S, Kaneko T, Kaplan OI, Teramoto T, Fujiwara M, Ishihara T, Blacque OE, Setou M
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Journal Title
Scientific Reports
Volume: 8
Issue: 1
Pages: 8392-8392
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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