| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In this study, we analyzed substrate recognition and regulatory mechanisms of protein kinases, as well as physiological and pathological functions. We identified the specific sequences of MYPT1, a substrate for Rho-kinase, as docking motifs (DMs) conferring selectivity and efficiency of phosphorylation by Rho-kinase. We also found that longer intrinsically disordered regions (IDRs) are preferably phosphorylated by stochastic simulation model using our screening dataset for 15 protein kinases. In addition, functional analyses revealed that MAPK-Npas4/MKL2 (mouse striatal neurons) and PKN-MKL1 (mouse heart) signals are involved in dopamine-induced gene expression and stress-induced cardiac dysfunction, respectively.
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