Regulation of meiosis

• Project/Area Number: 17K07392
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Chuo University
• Principal Investigator: Murakami Hiroshi 中央大学, 理工学部, 教授 (80262020)
• Project Period (FY): 2017-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 減数分裂 / 細胞周期 / 遺伝的組換え / チェックポイント / DNA複製 / 第一減数分裂 / 分裂酵母 / 転写因子 / 遺伝子組換え / 体細胞分裂 / 遺伝子発現 / 生物 / 発現制御

Outline of Final Research Achievements

Meiosis generates genetic diversity and is essential for gametogenesis, but there are many unclear points about its regulatory mechanism. In this study, we showed that Cds1 kinase phosphorylates the forkhead transcription factor Mei4 and that phosphorylation is required for checkpoints in the regulation of premeiotic DNA replication and initiation of recombination. Regarding the control mechanism of pre-meiotic DNA replication and first meiotic initiation, we showed that Cds1 phosphorylates Fkh2, a forkhead transcription factor, and that this phosphorylation is required for the checkpoint.

Academic Significance and Societal Importance of the Research Achievements

減数分裂は遺伝的多様性を生み出し、配偶子形成に必須であるがその制御機構に関しては不明な点が多い。減数分裂において、細胞周期が順序正しく進行するために、チェックポイント機構が必要である。しかし、このチェックポイント機構は、ほとんどの生物で未解明であった。この研究により、その一部が明らかにされた。

Research Products

• [Journal Article] Direct evaluation of cohesin-mediated sister kinetochore associations at meiosis I in fission yeast (2022)
  • Author(s): Masashi Nambu, Atsuki Kishikawa, Takatomi Yamada, Kento Ichikawa, Yunosuke Kira, Yuta Itabashi, Akira Honda, Kohei Yamada, Hiroshi Murakami, Ayumu Yamamoto
  • Journal Title: Journal of Cell Science Volume: 135 Issue: 1
  • DOI: 10.1242/jcs.259102
  • Peer Reviewed
• [Journal Article] The histone variant H2A.Z promotes initiation of meiotic recombination in fission yeast. (2018)
  • Author(s): Yamada S, Kugou K, Ding DQ, Fujita Y, Hiraoka Y, Murakami H, Ohta K, Yamada T.
  • Journal Title: Nucleic Acids Res. Volume: 46 Issue: 2 Pages: 609-620
  • DOI: 10.1093/nar/gkx1110
  • Peer Reviewed / Open Access
• [Journal Article] Correlation of Meiotic DSB Formation and Transcription Initiation Around Fission Yeast Recombination Hotspots. (2017)
  • Author(s): . Yamada, M. Okamura, H. Murakami, K.Ohta, T. Yamada
  • Journal Title: Genetics Volume: in press Issue: 2 Pages: 801-809
  • DOI: 10.1534/genetics.116.197954
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sulfur restriction extends fission yeast chronological lifespan through Ecl1 family genes by downregulation of ribosome (2017)
  • Author(s): Hokuto Ohtsuka*, Masahiro Takinami*, Takafumi Shimasaki, Takahide Hibi, Hiroshi Murakami, and Hirofumi Aiba
  • Journal Title: Molecular Microbiology Volume: 未定 Issue: 1 Pages: 84-97
  • DOI: 10.1111/mmi.13686
  • NAID: 120006349138
  • Peer Reviewed
• [Journal Article] Ecl1 is a zinc binding protein that is involved in the zinc-limitation dependent extension of chronological lifespan in fission yeast. (2017)
  • Author(s): Takafumi Shimasaki, Hokuto Ohtsuka, Chikako Naito, Takeshi Tenno, Hidekazu Hiroaki, Hiroshi Murakami, Hirofumi Aiba
  • Journal Title: Molecular Genetics and Genomics Volume: 292 Issue: 2 Pages: 475-481
  • DOI: 10.1007/s00438-016-1285-x
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] S. pombeでの減数分裂におけるFkh2の減数分裂前DNA複製と減数分 裂を連携するチェックポイント機構の解析 (2019)
  • Author(s): ◯岡﨑亜美 1、小菅清二 1、三輪由紀子 2、饗場浩文 2、村上浩士 1
  • Organizer: 酵母遺伝学フォーラム第 52 回研究報告会プログラム
• [Presentation] 分裂酵母における DNA 複製と相同組換え開始を制御する Cds1 キナー ゼと転写因子 Mei4 の機能解析 (2019)
  • Author(s): 小菅清二 1、山田貴富 1, 2、村上優子 3、饗場浩文 4、◯村上浩士 1
  • Organizer: 酵母遺伝学フォーラム第 52 回研究報告会プログラム
• [Presentation] Phosphorylation of Mei4 by Cds1 in the meiotic DNA replication checkpoint linking pre-meiotic DNA replication and meiotic recombination in fission yeast. (2019)
  • Author(s): Hiroshi Murakami1, Seiji Kosuge1, Takatomi Yamada1, Hirofumi Aiba2, Yuko Murakami3,
  • Organizer: 10th International Fission Yeast Meeting, Barcelona, Spain,
• [Presentation] Regulation of mei4+ expression during mitotic cell cycle in fission yeast (2017)
  • Author(s): Yuuki Akiya, Atsushi Ogihara, Hirofumi Aiba and Hiroshi Murakami
  • Organizer: 9th International Fission yeast meeting,
  • Int'l Joint Research
• [Presentation] 減数分裂時のDNA複製と相同組換えを連携するチェックポイント (2017)
  • Author(s): 小菅清二、山田貴富、饗場浩文、村上浩士
  • Organizer: 第５０回酵母遺伝学フォーラム研究報告会
• [Book] 化学と生物 (2018)
  • Author(s): 山田貴富、村上浩士、
  • Total Pages: 9
  • Publisher: 国際文献社