| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Rab1 was found to bind to the two distinct sites on GM130 and the Rab1 binding to GM130 was suggested to regulate the conformation of GM130 reading to the control of the higher-order assembly of GM130 and their association to the membrane. YIPF1 binds to proteins of 26kDa and 66kDa, YIPF2 binds to a 22kDa protein and YIPF6 binds to a 17kDa protein. Transiently expressed YIPF6 was found to be degraded by ERAD. YIPF proteins were found to be conserved in most of the eukaryotes and the 6 members of YIPF proteins were conserved widely in holozoa including animal cells. The analysis in human cells indicated that three distinct complexes are formed from two members of YIPF and localize in the upper, middle, and lower Golgi compartment. Therefore, in holozoa, YIPF proteins form complexes and differently localized at the Golgi apparatus.
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