| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Outline of Final Research Achievements |
In this research, I aim to explore roles of semaphori-6A, a signaling molecule implicated in axon guidance, in human embryonic stem cell (hESC) biology. For this end, I try to develop a conditional gene knockout system using a Crispr-cas9 genome editing tool.
Following the improved strategy, I established some hESC lines in which 2nd exon of SEME6A gene were flowed. I confirmed that lentivirus-mediated introduction of Cre recombinase induced deletion of 2nd exon and the expression semaphoring-6A protein decreased to the undetectable level. Preliminary data suggested that SAMA6A gene-disrupted hESCs show some abnormal phenotypes. This experimental platform allows us to genetically explore the function of a given gene that plays essential role in hESCs.
|
