Studies on the mechanism of polarized secretion of the hemostatic factor VWF in vascular endothelial cells.
| Project/Area Number |
17K07401
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
Yamazaki Yasuo 国立研究開発法人国立循環器病研究センター, 研究所, 室長 (30308621)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | オルガネラ形成 / 微小酸性環境 / 酸性オルガネラ / 微小環境形成 / 血管内皮細胞 / VWF / 細胞内輸送 / 細胞極性 / 細胞・組織 / シグナル伝達 / 生体分子 / 蛋白質 |
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| Outline of Final Research Achievements |
Hemostasis is essential to maintain the functional blood circulation; blood is required to clot in response to vascular damage to stop bleeding. Vascular endothelial cells are thin-layered cells covering luminal surface of the blood vessel. von Willebrand factor, a hemostatic protein, is produced in endothelial cells. It forms large homomeric multimers that are stored in specialized organelles called Weibel-Palade bodies. In this supported project, we studied a mechanism how the maturation and storage of von Willebrand factor is regulated in vascular endothelial cells. We found that a factor producing a local acidic environment contributes for the storage of von Willebrand factor in Weibel-Palade bodies.
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| Academic Significance and Societal Importance of the Research Achievements |
止血因子von Willebrand因子は止血反応に不可欠である。何らかの原因でvon Willebrand因子が機能しなくなると、von Willebrand病と呼ばれる血液凝固異常症を発症する。本研究の成果は、von Willebrand因子そのものが正常でも、その貯蔵機構に異常が生じるとvon Willebrand病になる可能性があることを示している。本研究課題により、これまで原因が不明であったvon Willebrand病の原因究明に貢献できる知見を得た。
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Report
(4 results)
Research Products
(5 results)
