Trimming of maternal mRNA during meiosis reinitiation
| Project/Area Number |
17K07405
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Ochanomizu University |
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Principal Investigator |
CHIBA KAZUYOSHI お茶の水女子大学, 基幹研究院, 教授 (70222130)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ヒトデ / mRNA / ウリジル化 / ポリＡ / 減数分裂 / ホルモン / 翻訳 / 母性 / 卵母細胞 / 母性mRNA / サイクリンＢ / オリゴＵ / トリミング / 分解 / ポリA鎖 / CPSF / 発生・分化 / 核酸 / 発現制御 |
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| Outline of Final Research Achievements |
Maternal cyclin B mRNAs with short poly(A) tails are uridylated in starfish oocytes. Generally, uridylated tails mediate mRNA decay, but starfish cyclin B mRNAs are not destructed in oocytes. In this study, we concluded that the activity of exoribonuclease removing uridines competes with poly(A) polymerase, causing trimming block and poly(A) elongation. In addition, we found that uridylation may be the signal determining decay or reuse. We published two papers in JBC showing intracellular pH regulation during meiosis resumption involved in poly(A) elongation.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、リボゾームタンパク質mRNAの3’末端の長いポリA鎖が、ホルモン刺激後に短縮された後に、新たに数塩基のオリゴUが結合すること、さらに驚くべきことに、胞胚期以降にオリゴUからポリA鎖が再伸長して、翻訳活性化することを明らかにした。すなわち、一度使われたmRNAのポリAが短縮し、再伸長することを明瞭に示した初めての例である。また、母性mRNAのU化意義が、動物胚の発生ステージ依存的に翻訳抑制からmRNA分解へと変わることも明らかにした。
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] SGK regulates pH increase and cyclin B-Cdk1 activation to resume meiosis in starfish ovarian oocytes.2019
Author(s)
Hosoda, E., Hiraoka, D., Hirohashi, N., Omi, S., Kishimoto, T. and Chiba, K.
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Journal Title
J Cell Biol.
Volume: 218
Issue: 11
Pages: 3612-3629
DOI
Related Report
Peer Reviewed / Open Access
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