| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
This study aimed to establish a technique for simultaneously imaging the nuclear localisation of a target gene using Dictyostelium that can visualise newly synthesised RNAs during cell differentiation. First, we constructed an efficient genome-editing system by creating high expression vectors of sgRNA using the endogenous promoter of tRNA. Next, based on this expression system, we combined it with dCas9, which abolishes nuclease activity, and SunTag, which aims to highlight the bright spots, to visualise the nuclear arrangement of the gene.
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