Study of the starting mechanisms of the first oscillatory wave for segmentation

• Project/Area Number: 17K07418
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Osaka Medical College
• Principal Investigator: Akiyama-Oda Yasuko 大阪医科大学, 医学部, 非常勤講師 (80426650)
• Co-Investigator(Kenkyū-buntansha): 小田 広樹 株式会社生命誌研究館, その他部局等, 主任研究員 (50396222)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 胚発生 / ゲノム / RNA-seq / ヘッジホッグシグナル / オオヒメグモ / パターン形成 / ヘッジホッグ / 体節形成 / 発現振動 / 発生・分化 / 遺伝子

Outline of Final Research Achievements

This study showed that three types of gene expression waves are responsible for segmental stripe formation in three regions of the spider body, head, thorax, and opisthosoma. msx1 is a new segmentation gene in the spider embryo; its expression is detected in all these waves and regulates dynamics of the waves. The newly identified primary wave of msx1, whose dynamics is regulated by Hedgehog signaling, splits into three stripes in the thorax and leads the oscillatory waves for the opisthosoma segmentation.

Academic Significance and Societal Importance of the Research Achievements

本研究では、オオヒメグモの縞パターンの形成が体の領域により２分割、３分割、振動という異なるダイナミクスの遺伝子発現の波により達成されることを示した。これらの異なるダイナミクスの波はコンポーネントを共有していることも示した。この発見はゲノム上に状況に応じて異なるダイナミクスを示す分子ネットワークが存在することを示している。このネットワークのさらなる解析が、コンテクストによる表現型の違いや、動物による縞パターン形成メカニズムの違いなどの理解へと発展しうると考えられる。

Research Products

• [Int'l Joint Research] Oxford Brookes University/University of Bristol(英国)
• [Int'l Joint Research] Georg-August-Universitat(ドイツ)
• [Int'l Joint Research] University of Wisconsin-Madison(米国)
• [Journal Article] Hedgehog signaling controls segmentation dynamics and diversity via msx1 in a spider embryo (2020)
  • Author(s): Akiyama-Oda Yasuko、Oda Hiroki
  • Journal Title: Science Advances Volume: 6 Issue: 37
  • DOI: 10.1126/sciadv.aba7261
  • Peer Reviewed / Open Access
• [Journal Article] オオヒメグモ胚のパターン形成─縞パターンはどのようにできるか？ (2020)
  • Author(s): 秋山-小田 康子
  • Journal Title: 生物の科学 遺伝 特集 クモのゲノム、遺伝子研究の最前線 Volume: 74 Pages: 670-676
• [Journal Article] The common house spider Parasteatoda tepidariorum. (2020)
  • Author(s): Oda, H., Akiyama-Oda, Y.
  • Journal Title: EvoDevo. Volume: 11 Pages: 6-6
  • Peer Reviewed / Open Access
• [Journal Article] Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum. (2020)
  • Author(s): Oda, H., Akiyama-Oda, Y.
  • Journal Title: Data Brief. Volume: 28 Pages: 105088-105088
  • DOI: 10.1016/j.dib.2019.105088
  • Peer Reviewed / Open Access
• [Journal Article] Microarray data on the comparison of transcript expression between normal and Pt-Delta RNAi embryos in the common house spider Parasteatoda tepidariorum. (2020)
  • Author(s): Oda, H., Akiyama-Oda, Y.
  • Journal Title: Data Brief. Volume: 25 Pages: 104350-104350
  • DOI: 10.1016/j.dib.2019.104350
  • Peer Reviewed / Open Access
• [Journal Article] Experimental duplication of bilaterian body axes in spider embryos: Holm's organizer and self-regulation of embryonic fields. (2019)
  • Author(s): Oda, H., Iwasaki-Yokozawa, S., Usui, T., Akiyama-Oda, Y.
  • Journal Title: Dev. Genes Evol. Volume: - Issue: 2 Pages: 49-63
  • DOI: 10.1007/s00427-019-00631-x
  • Peer Reviewed / Open Access
• [Journal Article] Homeobox gene duplication and divergence in arachnids. (2018)
  • Author(s): Leite, D.J., Baudouin-Gonzalez, L., Iwasaki-Yokozawa, S., Lozano-Fernandez, J., Turetzek, N., Akiyama-Oda, Y., Prpic, N.M., Pisani, D., Oda, H., Sharma, P.P., McGregor, A.P.
  • Journal Title: Mol Biol Evol. Volume: - Issue: 9 Pages: 2240-2253
  • DOI: 10.1093/molbev/msy125
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genome-scale embryonic developmental profile of gene expression in the common house spider Parasteatoda tepidariorum. (2018)
  • Author(s): Iwasaki-Yokozawa, S., Akiyama-Oda, Y., Oda, H.
  • Journal Title: Data Brief. Volume: 19 Pages: 865-867
  • DOI: 10.1016/j.dib.2018.05.106
  • Peer Reviewed / Open Access
• [Journal Article] A quantitative study of the diversity of stripe-forming processes in an arthropod cell-based field undergoing axis formation and growth (2018)
  • Author(s): Natsuki Hemmi, Yasuko Akiyama-Oda, Koichi Fujimoto, Hiroki Oda
  • Journal Title: Developmental Biology Volume: 437 Issue: 2 Pages: 84-104
  • DOI: 10.1016/j.ydbio.2018.03.001
  • Peer Reviewed / Open Access
• [Journal Article] The house spider genome reveals an ancient whole-genome duplication during arachnid evolution (2017)
  • Author(s): Schwager, E. E., Sharma, P. P., Clarke, T., Leite, D. J., Wierschin, T., Pechmann, M., Akiyama-Oda, Y., Esposito, L., Bechsgaard, J.,(35 authors omitted), Bucher, G., Gibbs, R. A., Coddington, J., Oda, H., Stanke, M., Ayoub, N.A., Prpic, N. M., Flot, J. F., Posnien, N., Richards, S., McGregor, A.
  • Journal Title: BMC Biology Volume: 15 Issue: 1 Pages: 62-62
  • DOI: 10.1186/s12915-017-0399-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evolutionary origin of type IV classical cadherins in arthropods. (2017)
  • Author(s): Sasaki M, Akiyama-Oda Y and Oda H
  • Journal Title: BMC Evol. Biol. Volume: 17 Issue: 1 Pages: 142-142
  • DOI: 10.1186/s12862-017-0991-2
  • Peer Reviewed / Open Access
• [Presentation] オオヒメグモ胚での全遺伝子発現パターンの推定： シングルセルRNAseq解析に基づく方法の確立 (2020)
  • Author(s): 赤岩孝憲、小田広樹、秋山-小田康子
  • Organizer: 日本動物学会第９１回大会
• [Presentation] Hedgehog signaling controls segmentation dynamics and diversity via msx1 in a spider embryo. (2019)
  • Author(s): Akiyama-Oda, Y., Oda, H.
  • Organizer: Society for Developmental Biology 78th Annual Meeting
  • Int'l Joint Research
• [Presentation] Segmentation dynamics and diversity controlled by Hedgehog signaling. (2019)
  • Author(s): Akiyama-Oda, Y., Oda, H.
  • Organizer: 52st JSDB
• [Presentation] Parasteatoda tepidariorum is a model spider the permits genome-based study of developmental biology. (2019)
  • Author(s): Akiyama-Oda, Y., Iwasaki-Yokozawa, S., Oda, H.
  • Organizer: 21st International Congress of Arachnology
  • Int'l Joint Research / Invited
• [Presentation] Wave generation mediated by Hedgehog signaling and its target gene: A key link between axis specification and segmentation. (2018)
  • Author(s): Akiyama-Oda, Y., Oda, H.
  • Organizer: Joint annual meeting of 51st JSDB and 70th JSCB
  • Int'l Joint Research
• [Presentation] A genome-wide study of Hedgehog signaling identifies a transcription factor gene mediating gene expression dynamics in the early spider embryo. (2017)
  • Author(s): Akiyama-Oda, Y., Iwasaki-Yokozawa, S., Oda, H.
  • Organizer: 18th International Congress of Developmental Biology (Singapore)
  • Int'l Joint Research
• [Presentation] A Hedgehog signaling network regulates the initiation of segmental oscillations in the early spider embryo. (2017)
  • Author(s): Akiyama-Oda, Y., Iwasaki-Yokozawa, S., Oda, H.
  • Organizer: 50th Annual Meeting of JSDB (Tokyo)
• [Remarks] BRH Data Resources
  • URL: https://www.brh2.jp
• [Remarks] Pt_spider Genome/Transcripts Database Home
  • URL: https://www.e-celldev.jp/pt_spider2/index.html
• [Remarks] BRH Data Resources
  • URL: https://www.brh2.jp/index.html
• [Remarks] BRH Data Resources
  • URL: http://www.brh2.jp/index.html