Study of the integration of spatio-temoporal information in vertebrate somitogenesis

• Project/Area Number: 17K07423
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Yabe Taijiro 基礎生物学研究所, 分子発生学研究部門, 助教 (30470074)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 体節形成 / 時空間情報 / ripply / 分節時計 / 組織形成 / ゼブラフィッシュ / 器官形成 / 分節形成

Outline of Final Research Achievements

In vertebrate, somitogenesis is known to be regulated by spatio-temporal information generated by cyclic activation of segmentation clock and posterior regression of wavefront respectively. In this study, I tried to identify the molecular mechanisms controlling the somite boundary formation in the downstream of segmentation clock and wavefront.
First, analysis of the phenotype of ripply1/ripply2 double mutant revealed that the significance of cessation of segmentation clock in somite boundary formation was less than previously predicted by theoretical model. Furthermore, I revealed that spatio-temporal information was integrated through the regulation fo ripply expression during zebrafish somitogenesis.

Academic Significance and Societal Importance of the Research Achievements

胚発生において、胚体内に形成される時空間情報をもとにして細胞の分化や増殖が厳密に制御されることにより、動物の複雑な体の構造は形成されると考えられているが、時間情報と空間情報が統合され個々の細胞の挙動を制御する仕組みについては不明な点が多く残されている。本研究において筆者は小型魚類であるゼブラフィッシュ の体節形成において分節時計とwavefrontによって作られる時空間情報が統合され、体節の分節境界を作る仕組みを明らかにした。分節時計と同じような仕組みは神経や筋肉の形成にも利用されていることが知られており、本研究の成果は脊椎動物の器官形成を広く理解するうえでも重要であると考えられる。

Research Products

• [Journal Article] Transcriptional autoregulation of zebrafish tbx6 is required for somite segmentation (2019)
  • Author(s): Ban Hiroyuki、Yokota Daisuke、Otosaka Shiori、Kikuchi Morimichi、Kinoshita Hirofumi、Fujino Yuuri、Yabe Taijiro、Ovara Hiroki、Izuka Ayaka、Akama Kagari、Yamasu Kyo、Takada Shinji、Kawamura Akinori
  • Journal Title: Development Volume: 146 Pages: 1-11
  • DOI: 10.1242/dev.177063
  • Peer Reviewed / Open Access
• [Journal Article] Functional roles of the Ripply-mediated suppression of segmentation gene expression at the anterior presomitic mesoderm in zebrafish (2018)
  • Author(s): Kinoshita Hirofumi、Ohgane Nanae、Fujino Yuuri、Yabe Taijiro、Ovara Hiroki、Yokota Daisuke、Izuka Ayaka、Kage Daichi、Yamasu Kyo、Takada Shinji、Kawamura Akinori
  • Journal Title: Mechanisms of Development Volume: 152 Pages: 21-31
  • DOI: 10.1016/j.mod.2018.06.001
  • Peer Reviewed
• [Presentation] Molecular mechanisms for the positioning of somite boundaries in zebrafish (2019)
  • Author(s): 矢部泰二郎 高田慎治
  • Organizer: 14th International Zebrafish Conference
  • Int'l Joint Research
• [Presentation] “Molecular mechanisms for the positioning of somite boundaries in zebrafish” (2018)
  • Author(s): Taijiro Yabe and Shinji Takada
  • Organizer: oint Annual Meeting of 51st JSDB and 70th JSCB
• [Presentation] “The molecular mechanism for the termination of segmentation clock during zebrafish somitogenesis” (2018)
  • Author(s): Taijiro Yabe and Shinji Takada
  • Organizer: 3th International Zebrafish Conference
  • Int'l Joint Research
• [Presentation] “Molecular mechanisms for the positioning of somite boundaries in zebrafish” (2018)
  • Author(s): Taijiro Yabe and Shinji Takada
  • Organizer: 第24回小型魚類研究会
• [Presentation] 『ゼブラフィッシュ体節形成における時空間情報の統合機構』 (2018)
  • Author(s): 矢部泰二郎、高田慎治
  • Organizer: JSDB秋季シンポジウム2018
• [Presentation] Molecular mechanism to convert the segmentation clock into the segmental pattern of somites in the zebrafish (2017)
  • Author(s): 矢部泰二郎、高田慎治
  • Organizer: 50th Annual Meeting of JSDB