Functional analysis of LIL proteins that have chlorophyll-binding motifs, thylakoid localized and low-temperature responsive

• Project/Area Number: 17K07431
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Hokkaido University
• Principal Investigator: Tanaka Ryouichi 北海道大学, 低温科学研究所, 准教授 (20311516)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 光合成 / 低温

Outline of Final Research Achievements

To elucidate the mechanism by which plants in the cryosphere avoid photodamages at low temperatures, we sought to understand the function of light-harvesting-like proteins (LILs) with chlorophyll-binding motifs, which are thought to function in the regulation and protection of the photosystem. Analysis of Arabidopsis LIL8 mutants and complexes, as well as time-resolved chlorophyll fluorescence measurements, suggested that LIL8 is involved in the regulation of light-harvesting system dynamics. Iso-seq analysis and immunoblotting in yew showed an inverse correlation between annual changes in photosystem II quantum yield and LIL1 accumulation, suggesting that LIL1 binds to photosystem II in winter and is involved in heat dissipation.

Academic Significance and Societal Importance of the Research Achievements

寒冷圏の植物、とくに樹木は寒冷圏の生態系のみならず、地球全体の生態系を支える上で重要な役割を果たしている。しかし、物質生産の原動力となる光合成の反応は気温の影響を受けやすく、とくに低温下では、光合成に関わるタンパク質や葉緑体が障害を受けやすいことが知られている。寒冷圏の植物は他の地域の植物に比べて低温下で光合成のタンパク質を守る機能が発達していると考えられる。本研究は、このLILというタンパク質ファミリーの研究を通して、低温下で光合成を守る機能の解明に貢献した。

Research Products

• [Journal Article] Subcellular localization of chlorophyllase2 reveals it is not involved in chlorophyll degradation during senescence in Arabidopsis thalina. (2020)
  • Author(s): Hu X, Ting J, Hortensteiner S, Tanaka A, Tanaka R
  • Journal Title: Plant Science Volume: 290 Pages: 110314-110314
  • DOI: 10.1016/j.plantsci.2019.110314
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Deficiency of the Stroma-Lamellar Protein LIL8/PSB33 Affects Energy Transfer Around PSI in Arabidopsis (2017)
  • Author(s): Kato Yukako、Yokono Makio、Akimoto Seiji、Takabayashi Atsushi、Tanaka Ayumi、Tanaka Ryouichi
  • Journal Title: Plant and Cell Physiology Volume: 58 Issue: 11 Pages: 2026-2039
  • DOI: 10.1093/pcp/pcx124
  • NAID: 120006533129
  • Peer Reviewed
• [Presentation] Correlation of winter-specific gene expression and sustained thermal dissipation in over-wintering yew leaves (2021)
  • Author(s): Zihao Ye, Mina Sawada, Ryo Moriyama, Toshihiko Hara, Ayumi Tanaka, Atsushi Takabayashi, Ryouichi Tanaka
  • Organizer: 日本植物生理学会第62回年会
• [Presentation] 秋から冬にかけてのイチ イの光化学系 II 量子収率 の漸次的変化 (2020)
  • Author(s): 澤田 未菜，岩佐 万紀子，森山 亮，原 登志彦，田中 歩，伊藤 寿，高林 厚史，田中 亮一
  • Organizer: 日本植物学会第84回大会
• [Presentation] A stable intermediate pigment-protein complex for photosystem II assembly. (2019)
  • Author(s): Ryouichi Tanaka
  • Organizer: First Japan-US Binational Seminar
  • Int'l Joint Research / Invited
• [Presentation] Multiple roles of light-harvesting-like proteins in the regulation and maintenance of the photosynthetic machinery (2018)
  • Author(s): Ryouichi Tanaka
  • Organizer: Japan-Finland Seminar on "Shaping photosynthesis against climate change and toward efficient water and nutrient management"
  • Int'l Joint Research / Invited
• [Presentation] ストロマラメラに局在するLight-harvesting-likeタンパク質LIL8の機能解析 (2017)
  • Author(s): Yukako Kato, Makio Yokono, Seiji Akimoto, Atsushi Takabayashi, Ayumi Tanaka, Ryouichi Tanaka
  • Organizer: 日本植物学会第81回大会
• [Remarks] 北海道大学・低温科学研究所・生物適応科学ホームページ
  • URL: http://www.lowtem.hokudai.ac.jp/plantadapt/