Study of the molecular mechanisms and evolution of GUN1 plastid signaling

• Project/Area Number: 17K07444
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: Mochizuki Nobuyoshi 京都大学, 理学研究科, 助教 (60280939)
• Project Period (FY): 2017-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: プラスチドレトログレードシグナル / 葉緑体 / PPR / 色素体機能 / レトログレードシグナル / 光合成 / PPRタンパク / 天然変性領域 / 植物陸上化 / 色素体機能・光合成 / プラスチドシグナル

Outline of Final Research Achievements

Photosynthesis-related genes encoded in the nuclear genomes are co-ordinately regulated by GUN-signal transduction pathways. Novel approaches were conducted to identify factors involved in GUN plastid signaling. We identify genes encoding proteins related to the plastid tRNA modification and cytosolic carboxypeptidase activity. (2) Analysis of GUN1 orthologues derived from Chara braunii, Marchantia polymorpha and Arabidopsis thaliana revealed that GUN1 first evolved the function in the plastid-dependent regulation on nuclear genes. GUN1 involved in RNA-editing after the plant colonization to the land. GUN1-depnendent regulation of plastid translation was evolved at latest in angiosperm.

Academic Significance and Societal Importance of the Research Achievements

GUNシグナルを初めとするレトログレードシグナルについては、ハイインパクトな雑誌に報告が相次いでいるが、シグナルの実体や分子機構の詳細は不明である。また、植物の緑化はフィトクロムなど光受容体が主に制御すると考えられてきたが、プラスチドシグナルも大きな役割を果たす事が分かりつつあり、本研究で緑化制御の新たな理解が進むと期待される。PPRについては、GUN1が属するPPR-SMRタンパクは未知の部分が多く、本研究による分子機構解明がそのブレイクスルーになる。GUNシグナルの進化について理解が進めば、植物陸上化におけるGUN1シグナルとプラスチド進化のつながりと生物学的意義が明らかになる。

Research Products

• [Int'l Joint Research] University of Southampton(英国)
• [Int'l Joint Research] Ludwig Maximilians Universitat Munchen(ドイツ)
• [Int'l Joint Research] University of Southampton(英国)
• [Int'l Joint Research] Ludwig-Maximilians-University(ドイツ)
• [Int'l Joint Research] University of Southampton/University of Cambridge(英国)
• [Journal Article] The retrograde signaling protein GUN1 regulates tetrapyrrole biosynthesis (2019)
  • Author(s): Shimizu, T. Kacprzak, S. M. Mochizuki, N. Nagatani, A. Watanabe, S. Shimada, T. Tanaka, K. Hayashi, Y. Arai, M. Leister, D. Okamoto, H. Terry, M. J. Masuda, T.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 116 Pages: 24900-24906
  • DOI: 10.1101/532036
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Auxin Contributes to the Intraorgan Regulation of Gene Expression in Response to Shade. (2018)
  • Author(s): Kim S, Mochizuki N, Deguchi A, Nagano AJ, Suzuki T, Nagatani A.
  • Journal Title: Plant Physiology Volume: 177 Issue: 2 Pages: 847-862
  • DOI: 10.1104/pp.17.01259
  • Peer Reviewed
• [Journal Article] Plastid-to-Nucleus Retrograde Signalling during Chloroplast Biogenesis Does Not Require ABI4. (2018)
  • Author(s): Kacprzak SM, Mochizuki N, Naranjo B, Xu D, Leister D, Kleine T, Okamoto H, Terry MJ.
  • Journal Title: Plant Physiology Volume: 179 Issue: 1 Pages: 18-23
  • DOI: 10.1104/pp.18.01047
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Seedlings Lacking the PTM Protein Do Not Show a genomes uncoupled (gun) Mutant Phenotype (2017)
  • Author(s): Page Mike T.、Kacprzak Sylwia M.、Mochizuki Nobuyoshi、Okamoto Haruko、Smith Alison G.、Terry Matthew J.
  • Journal Title: Plant Physiology Volume: 174 Issue: 1 Pages: 21-26
  • DOI: 10.1104/pp.16.01930
  • Peer Reviewed / Int'l Joint Research
• [Presentation] プラスチドシグナルとＧＵＮ１機能の進化 (2020)
  • Author(s): 望月伸悦、坂山英俊、西山智明、長谷あきら
  • Organizer: 第６１回日本植物生理学会年会
• [Presentation] RNA-seqによる排水組織特異的な発現遺伝子の同定 (2020)
  • Author(s): 八木宏樹、永野惇、田村謙太郎、望月伸悦、長谷あきら、嶋田知生
  • Organizer: 第６１回日本植物生理学会年会
• [Presentation] GlobalProliferativeArrestに関わるシロイヌナズナFIREWORKS遺伝子の同定 (2019)
  • Author(s): 今井翔，廣澤ひかる，鈴木孝征，小鍛治敬生，望月伸悦，長谷あきら，上口智治
  • Organizer: 日本植物生理学会 第６０回日本植物生理学会年会
• [Presentation] シロイヌナズナ実生の脱黄化における葉 緑体シグナル伝達系の解析 (2019)
  • Author(s): 望月伸悦，長谷あきら
  • Organizer: 日本植物生理学会 第６０回日本植物生理学会年会
• [Presentation] GUN1 regulates tetrapyrrole biosynthesis in Arabidopsis (2019)
  • Author(s): Tatsuru Masuda, Takayuki Shimizu, Nobuyoshi Mochizuki, Akira Nagatani, Satoru Watanabe, Kacprzak Sylwia, Haruko Okamoto, Terry Matthew
  • Organizer: 日本植物生理学会 第６０回日本植物生理学会年会
• [Presentation] 強い陰環境におけるphyA依存的な避陰応答抑制に関する研究 (2019)
  • Author(s): 望月 伸悦, 鈴木 友美, 長谷 あきら
  • Organizer: 日本植物生理学会 第６０回日本植物生理学会年会
• [Presentation] Re-evaluation of the role of ABI4 in plastid-to-nucleus retrograde signalling during chloroplast biogenesis (2018)
  • Author(s): Nobuyoshi Mochizuki, Sylwia M. Kacprzak, Belen Naranjo, Duorong Xu, Dario Leister, Tatjana Kleine, Haruko Okamoto, and Matthew J. Terry
  • Organizer: Retrograde signalling from endosymbiotic organelles
  • Int'l Joint Research
• [Presentation] ABI4 is not required for plastid-to-nucleus GUN retrograde signalling during chloroplast biogenesis (2018)
  • Author(s): Nobuyoshi Mochizuki, Sylwia M. Kacprzak, Belen Naranjo, Duorong Xu, Dario Leister, Tatjana Kleine, Haruko Okamoto, and Matthew J. Terry
  • Organizer: International Symposium on Photosynthesis and Chloroplast Biogenesis 2018
  • Int'l Joint Research
• [Presentation] 脱黄化制御におけるGUN1シグナル伝達系の解析 (2017)
  • Author(s): 望月伸悦、長谷あきら
  • Organizer: 日本植物生理学会
• [Remarks] 植物生理学（長谷研究室）
  • URL: http://physiol2.bot.kyoto-u.ac.jp/HP3/
• [Remarks] 研究室ホームページ
  • URL: http://physiol2.bot.kyoto-u.ac.jp/HP3/
• [Remarks] プラスチドシグナル
  • URL: http://physiol2.bot.kyoto-u.ac.jp/HP3/page1-4%20plastid.html