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Study of the molecular mechanisms and evolution of GUN1 plastid signaling

Research Project

Project/Area Number 17K07444
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionKyoto University

Principal Investigator

Mochizuki Nobuyoshi  京都大学, 理学研究科, 助教 (60280939)

Project Period (FY) 2017-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsプラスチドレトログレードシグナル / 葉緑体 / PPR / 色素体機能 / レトログレードシグナル / 光合成 / PPRタンパク / 天然変性領域 / 植物陸上化 / 色素体機能・光合成 / プラスチドシグナル
Outline of Final Research Achievements

Photosynthesis-related genes encoded in the nuclear genomes are co-ordinately regulated by GUN-signal transduction pathways. Novel approaches were conducted to identify factors involved in GUN plastid signaling. We identify genes encoding proteins related to the plastid tRNA modification and cytosolic carboxypeptidase activity. (2) Analysis of GUN1 orthologues derived from Chara braunii, Marchantia polymorpha and Arabidopsis thaliana revealed that GUN1 first evolved the function in the plastid-dependent regulation on nuclear genes. GUN1 involved in RNA-editing after the plant colonization to the land. GUN1-depnendent regulation of plastid translation was evolved at latest in angiosperm.

Academic Significance and Societal Importance of the Research Achievements

GUNシグナルを初めとするレトログレードシグナルについては、ハイインパクトな雑誌に報告が相次いでいるが、シグナルの実体や分子機構の詳細は不明である。また、植物の緑化はフィトクロムなど光受容体が主に制御すると考えられてきたが、プラスチドシグナルも大きな役割を果たす事が分かりつつあり、本研究で緑化制御の新たな理解が進むと期待される。PPRについては、GUN1が属するPPR-SMRタンパクは未知の部分が多く、本研究による分子機構解明がそのブレイクスルーになる。GUNシグナルの進化について理解が進めば、植物陸上化におけるGUN1シグナルとプラスチド進化のつながりと生物学的意義が明らかになる。

Report

(5 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (21 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (5 results) Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (9 results) (of which Int'l Joint Research: 2 results) Remarks (3 results)

  • [Int'l Joint Research] University of Southampton(英国)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] Ludwig Maximilians Universitat Munchen(ドイツ)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] University of Southampton(英国)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] Ludwig-Maximilians-University(ドイツ)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] University of Southampton/University of Cambridge(英国)

    • Related Report
      2017 Research-status Report
  • [Journal Article] The retrograde signaling protein GUN1 regulates tetrapyrrole biosynthesis2019

    • Author(s)
      Shimizu, T. Kacprzak, S. M. Mochizuki, N. Nagatani, A. Watanabe, S. Shimada, T. Tanaka, K. Hayashi, Y. Arai, M. Leister, D. Okamoto, H. Terry, M. J. Masuda, T.
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 116 Pages: 24900-24906

    • DOI

      10.1101/532036

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Auxin Contributes to the Intraorgan Regulation of Gene Expression in Response to Shade.2018

    • Author(s)
      Kim S, Mochizuki N, Deguchi A, Nagano AJ, Suzuki T, Nagatani A.
    • Journal Title

      Plant Physiology

      Volume: 177 Issue: 2 Pages: 847-862

    • DOI

      10.1104/pp.17.01259

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Plastid-to-Nucleus Retrograde Signalling during Chloroplast Biogenesis Does Not Require ABI4.2018

    • Author(s)
      Kacprzak SM, Mochizuki N, Naranjo B, Xu D, Leister D, Kleine T, Okamoto H, Terry MJ.
    • Journal Title

      Plant Physiology

      Volume: 179 Issue: 1 Pages: 18-23

    • DOI

      10.1104/pp.18.01047

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Seedlings Lacking the PTM Protein Do Not Show a genomes uncoupled (gun) Mutant Phenotype2017

    • Author(s)
      Page Mike T.、Kacprzak Sylwia M.、Mochizuki Nobuyoshi、Okamoto Haruko、Smith Alison G.、Terry Matthew J.
    • Journal Title

      Plant Physiology

      Volume: 174 Issue: 1 Pages: 21-26

    • DOI

      10.1104/pp.16.01930

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] プラスチドシグナルとGUN1機能の進化2020

    • Author(s)
      望月伸悦、坂山英俊、西山智明、長谷あきら
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] RNA-seqによる排水組織特異的な発現遺伝子の同定2020

    • Author(s)
      八木宏樹、永野惇、田村謙太郎、望月伸悦、長谷あきら、嶋田知生
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] GlobalProliferativeArrestに関わるシロイヌナズナFIREWORKS遺伝子の同定2019

    • Author(s)
      今井翔,廣澤ひかる,鈴木孝征,小鍛治敬生,望月伸悦,長谷あきら,上口智治
    • Organizer
      日本植物生理学会 第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] シロイヌナズナ実生の脱黄化における葉 緑体シグナル伝達系の解析2019

    • Author(s)
      望月伸悦,長谷あきら
    • Organizer
      日本植物生理学会 第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] GUN1 regulates tetrapyrrole biosynthesis in Arabidopsis2019

    • Author(s)
      Tatsuru Masuda, Takayuki Shimizu, Nobuyoshi Mochizuki, Akira Nagatani, Satoru Watanabe, Kacprzak Sylwia, Haruko Okamoto, Terry Matthew
    • Organizer
      日本植物生理学会 第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 強い陰環境におけるphyA依存的な避陰応答抑制に関する研究2019

    • Author(s)
      望月 伸悦, 鈴木 友美, 長谷 あきら
    • Organizer
      日本植物生理学会 第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Re-evaluation of the role of ABI4 in plastid-to-nucleus retrograde signalling during chloroplast biogenesis2018

    • Author(s)
      Nobuyoshi Mochizuki, Sylwia M. Kacprzak, Belen Naranjo, Duorong Xu, Dario Leister, Tatjana Kleine, Haruko Okamoto, and Matthew J. Terry
    • Organizer
      Retrograde signalling from endosymbiotic organelles
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] ABI4 is not required for plastid-to-nucleus GUN retrograde signalling during chloroplast biogenesis2018

    • Author(s)
      Nobuyoshi Mochizuki, Sylwia M. Kacprzak, Belen Naranjo, Duorong Xu, Dario Leister, Tatjana Kleine, Haruko Okamoto, and Matthew J. Terry
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogenesis 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 脱黄化制御におけるGUN1シグナル伝達系の解析2017

    • Author(s)
      望月伸悦、長谷あきら
    • Organizer
      日本植物生理学会
    • Related Report
      2017 Research-status Report
  • [Remarks] 植物生理学(長谷研究室)

    • URL

      http://physiol2.bot.kyoto-u.ac.jp/HP3/

    • Related Report
      2019 Research-status Report
  • [Remarks] 研究室ホームページ

    • URL

      http://physiol2.bot.kyoto-u.ac.jp/HP3/

    • Related Report
      2018 Research-status Report
  • [Remarks] プラスチドシグナル

    • URL

      http://physiol2.bot.kyoto-u.ac.jp/HP3/page1-4%20plastid.html

    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2024-01-30  

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