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Analysis of reproductive process by calcium imaging system

Research Project

Project/Area Number 17K07446
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionKyoto University (2018-2020)
Osaka University (2017)

Principal Investigator

Iwano Megumi  京都大学, 生命科学研究科, 研究員 (50160130)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsカルシウム / 情報伝達系 / イメージング / シロイヌナズナ / ゼニゴケ / 生殖 / カルシウムセンサー / 苔類ゼニゴケ / 生殖過程 / ライブセルイメージング / 精子誘引 / 受精 / カルシウムイメージング / 発光イメージング / カルシウムシグナリング / オプトジェネティクス / 自家不和合性 / 植物
Outline of Final Research Achievements

The reproductive process of angiosperms leads to fertilization through pollination and attraction of pollen tubes to ovules. In the liverwort, a base land plant, sperm from the sperm genitals are attracted to the egg maker and lead to fertilization. In these reproductive processes, calcium-mediated signal transduction system is considered to be involved in communication between male and female cells. In this study, to elucidate molecules functioning in this system, we developed multicolored calcium sensors with different affinity for calcium ion and created plants capable of sensing the calcium dynamics of cytoplasm and organelles. Physiological and genetic analysis of the plants searched for calcium transporter genes that function during reproductive processes.

Academic Significance and Societal Importance of the Research Achievements

生体内のCa2+は、セカンドメッセンジャーとして細胞の増殖、生殖、環境応答などの生理反応に関与する。Ca2+濃度の低下は、染色体の凝縮や動原体微小管の安定化を阻害し細胞分裂周期の遅延や異常を引き起こす。また、植物が機械刺激などのストレスを感知すると、細胞外からのCa2+流入または液胞、小胞体などのカルシウムストアからCa2+放出が起き、機械刺激応答が発動される。従って、カルシウム動態を詳細に解析し、Ca2+カルシウム情報伝達系で機能するCa輸送体を解明することは、植物の生理反応全般の理解に必要であり、将来的には植物の生産性の向上に繋がる。

Report

(4 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (8 results)

All 2020 2019 2018 2017

All Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (2 results) (of which Invited: 1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Kinase in the liverwort Marchantia polymorpha; A photosynthesis-dependent kinase for carbon metabolism2020

    • Author(s)
      Koide E, Suetsugu N, Iwano M, Gotoh E, Nomura Y, Stolze SC, Nakagami H, Kohchi T, Nishihama R
    • Journal Title

      Plant Cell & Physiology

      Volume: 61 Issue: 3 Pages: 631-643

    • DOI

      10.1093/pcp/pcz232

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Parallel evolution of dominant pistil-side self-incompatibility suppressors in Arabidopsis2020

    • Author(s)
      Fujii Sota、Shimosato-Asano Hiroko、Kakita Mitsuru、Kitanishi Takashi、Iwano Megumi、Takayama Seiji
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 1404-1404

    • DOI

      10.1038/s41467-020-15212-0

    • NAID

      120006840746

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A stigmatic gene confers interspecies incompatibility in the Brassicaceae2019

    • Author(s)
      Fujii Sota、Tsuchimatsu Takashi、Kimura Yuka、Ishida Shota、Tangpranomkorn Surachat、Shimosato-Asano Hiroko、Iwano Megumi、Furukawa Shoko、Itoyama Wakana、Wada Yuko、Shimizu Kentaro K.、Takayama Seiji
    • Journal Title

      Nature Plants

      Volume: 5 Issue: 7 Pages: 731-741

    • DOI

      10.1038/s41477-019-0444-6

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Bioluminescent Low-Affinity Ca2+ Indicator for ER with Multicolor Calcium Imaging in Single Living Cells2018

    • Author(s)
      Hossain Md Nadim、Suzuki Kazushi、Iwano Megumi、Matsuda Tomoki、Nagai Takeharu
    • Journal Title

      ACS Chemical Biology

      Volume: 13 Issue: 7 Pages: 1862-1871

    • DOI

      10.1021/acschembio.7b01014

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 苔類ゼニゴケMCAの成長・生殖過程における機能解析2019

    • Author(s)
      岩野 恵
    • Organizer
      日本植物学会第 83 回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] ライブイメージングのための高光度発光植物の開発2017

    • Author(s)
      岩野恵、永井健治
    • Organizer
      第69回日本細胞生物学会大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Patent(Industrial Property Rights)] 発光蛋白質、その基質、及びそれらの使用2018

    • Inventor(s)
      永井健冶、岩野恵
    • Industrial Property Rights Holder
      永井健冶、岩野恵
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-106866
    • Filing Date
      2018
    • Related Report
      2018 Research-status Report
  • [Patent(Industrial Property Rights)] デバイス、及びそれを用いた判定システム2017

    • Inventor(s)
      永井健治、新井由之、岩野恵
    • Industrial Property Rights Holder
      永井健治、新井由之、岩野恵
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Research-status Report
    • Overseas

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Published: 2017-04-28   Modified: 2022-12-28  

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