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Studies on photoperiodic regulation of flowering time: altering a long-day plant to a short-day plant

Research Project

Project/Area Number 17K07452
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionInternational Christian University

Principal Investigator

MIZOGUCHI TSUYOSHI  国際基督教大学, 教養学部, 教授 (70281623)

Project Period (FY) 2017-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsphotoperiodic response / 光周期 / 生物時計 / 概日リズム / シロイヌナズナ / 長日性 / 短日性 / 光周性 / Arabidopsis thaliana / 概日時計遺伝子 / 概日時計 / 植物科学
Outline of Final Research Achievements

FT expression was higher in short-day than long-day condition in elf3;gi. FT expression level under short-day condition was decreased by night-break. In addition to Ler background, elf3;gi mutant candidates in Ws and Col backgrounds were screened, identified and characterized.elf3;gi in Ler background exhibited an early flowering phenotype under short-day in natural field condition, in a similar way to that in laboratory condition.elf3 and gi mutant seeds of Brassica rapa were obtained. These mutant seeds together with control were sown and flowering time of these plants were investigated. elf3 and gi mutants in Brassica rapa were crossed to produce elf3;gi double mutants.

Academic Significance and Societal Importance of the Research Achievements

長日性のシロイヌナズナを基本型として、多種類の変異導入により、短日性を含めた自然界に存在するさまざまな光周期応答性の再現(再構成)を試みる第一歩となった。短日性を示すシロイヌナズナ変異体が実際に得られたことで、光周期応答性の分子基盤の解明につながる可能性がある。本研究により得られた研究成果により、今後は植物の開花・種子収穫季節を自在に制御する技術開発につながる可能性が考えられる。光周期応答性を改変した新規植物の分子育種により、地球上で今後さらに劣化の進行が危惧される栽培環境への適応可能性が拡大され、食糧生産性向上と地球環境保全の基礎基盤作りに活用されると考えられる。

Report

(7 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (16 results)

All 2021 2020 2019 2018 2017

All Journal Article (5 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (11 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato2021

    • Author(s)
      Kentaro Mori, Martine Lemaire-Chamley, Joana Jorly, Fernando Carrari, Mariana Conte, Erika Asamizu, Tsuyoshi Mizoguchi, Hiroshi Ezura, Christophe Rothan
    • Journal Title

      Journal of Experimental Botany

      Volume: 72 Issue: 4 Pages: 1181-1197

    • DOI

      10.1093/jxb/eraa495

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Temporal restriction of salt inducibility in expression of salinity-stress related gene by the circadian clock in <i>Solanum lycopersicum</i>2019

    • Author(s)
      Kelsey Coyne, Melissa Mullen Davis, Tsuyoshi Mizoguchi, Ryosuke Hayama
    • Journal Title

      Plant Biotechnology

      Volume: 36 Issue: 3 Pages: 195-200

    • DOI

      10.5511/plantbiotechnology.19.0703a

    • NAID

      130007724759

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2019-09-25
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ubiquitin carboxyl-terminal hydrolases are required for period maintenance of the circadian clock at high temperature in Arabidopsis2019

    • Author(s)
      Ryosuke Hayama, Peizhen Yang, Federico Valverde, Tsuyoshi Mizoguchi, Ikuyo Furutani-Hayama, Richard D. Vierstra, and George Coupland
    • Journal Title

      Scientific Reports

      Volume: 19;9(1) Issue: 1 Pages: 17030-17030

    • DOI

      10.1038/s41598-019-53229-8

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evaluation of internal control genes for quantitative realtime PCR analyses for studying fruit development of dwarf tomato cultivar ‘Micro-Tom’2018

    • Author(s)
      Choi SW, Hoshikawa K, Fujita S, Thi DP, Mizoguchi T, Ezura H, Ito E.
    • Journal Title

      Plant Biotechnology

      Volume: 35 Issue: 3 Pages: 225-235

    • DOI

      10.5511/plantbiotechnology.18.0525a

    • NAID

      130007495570

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2018-09-25
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Differential effects of light-to-dark transitions on phase setting in circadian expression among clock-controlled genes in Pharbitis nil.2018

    • Author(s)
      Hayama R, Mizoguchi T, Coupland G.
    • Journal Title

      Plant Signal. Behav.

      Volume: 35 Issue: 6 Pages: 225-235

    • DOI

      10.1080/15592324.2018.1473686

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] シロイヌナズナ(Columbia)の短日型光周性を有する変異体のスクリーニング2021

    • Author(s)
      溝口剛
    • Organizer
      日本植物バイオテクノロジー学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 恒明条件でのジベレリン依存型花成制御におけるPTA7の役割2020

    • Author(s)
      坂本絢、宮田佳奈、早山良輔、溝口剛
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Differential effects of light-to-dark transitions on phase setting in circadian expression among clock-controlled genes in Pharbitis nil.2019

    • Author(s)
      Hayama R, Mizoguchi T, Coupland G.
    • Organizer
      The 60th of Annual meeting of the Japanese Society of Plant Physiologists
    • Related Report
      2018 Research-status Report
  • [Presentation] Inversion of photoperiodic response by mutations of circadian clock genes in Arabidopsis thaliana.2018

    • Author(s)
      Murayama Y, Kuroki K, Hara M, Miyata K, Hayama R, Ito E, Choi SW, Mizoguchi T.
    • Organizer
      International Symposium on Biological Material Science -Aiming at Future Interdiciplinary Collaborations
    • Related Report
      2018 Research-status Report
  • [Presentation] Gibberellic acid and PTA7 may control flowering time and stem elongation via a common genetic pathway in Arabidopsis thaliana.2018

    • Author(s)
      Sakamoto A, Miyara K, Kobayashi M, Hayama R, Ito E, Choi SW, Misoguchi T.
    • Organizer
      International Symposium on Biological Material Science -Aiming at Future Interdiciplinary Collaborations
    • Related Report
      2018 Research-status Report
  • [Presentation] early flowering 3 (elf3);gigantea (gi) の短日性花成応答形質に関する解析2018

    • Author(s)
      村山優子、黒木健、原美由紀、宮田佳奈、早間良輔、伊藤瑛海、崔勝媛、溝口剛
    • Organizer
      第36回日本植物細胞分子生物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 恒明条件におけるlhy;cca1変異形質を増強するpta7変異の解析2018

    • Author(s)
      坂本絢、宮田佳奈、小林桃佳、早間良輔、伊藤瑛海、崔勝媛、溝口剛
    • Organizer
      第36回日本植物細胞分子生物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] マイクロトム果実形成と品質維持における液胞輸送の役割2018

    • Author(s)
      伊藤瑛海、崔勝媛、星川健、溝口剛、江面浩
    • Organizer
      第134回日本育種学会講演会、第60回シンポジウム
    • Related Report
      2018 Research-status Report
  • [Presentation] PSEUDO RESPONSE REGULATORs stabilize CONSTANS protein to promote flowering in response to day length2018

    • Author(s)
      Ryosuke Hayama, Liron Sarid-Krebs, Rene Richter, Virginia Fernandez, Tsuyoshi Mizoguchi, George Coupland
    • Organizer
      International Symposium on Plant Photobiology, 2018
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] PSEUDO RESPONSE REGULATORs stabilize CONSTANS protein to promote flowering in response to day length2018

    • Author(s)
      Ryosuke Hayama, Liron Sarid-Krebs, Rene Richter, Virginia Fernandez, Tsuyoshi Mizoguchi, George Coupland
    • Organizer
      The 59th Annual Meeting of the Japanese Society of Plant Physiologists
    • Related Report
      2017 Research-status Report
  • [Presentation] Inversion of photoperiodic response by mutations of circadian clock genes in Arabidopsis thaliana2017

    • Author(s)
      Tsuyoshi Mizoguchi
    • Organizer
      Tunisia-Japan Symposium on Science, Society and Technology (TJASSST 2017)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2017-04-28   Modified: 2024-01-30  

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