| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
An Arabidopsis mutant named defective repression of OLE3::LUC 1 (drol1) was originally isolated as a mutant with defects in the repression of OLEOSIN gene after seed germination. Since the DROL1 gene was speculated to encode a splicing factor, the transcriptome of drol1 mutant was compared to that of the wild type. The analysis revealed that the introns with AT-AC terminal sequences were specifically retained in the transcriptome of drol1, indicating that DROL1 was required for the splicing of AT-AC-type introns. This result suggested the existence of new subclasses spliceosome specifically excising introns with AT-AC terminal dinucleotides.
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