Mechanism on diversity and adaptability of plant peroxisome

• Project/Area Number: 17K07457
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Mano Shoji 基礎生物学研究所, オルガネラ制御研究室, 准教授 (20321606)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ペルオキシソーム / 多様性 / シロイヌナズナ / apem変異体 / タンパク質輸送 / ゼニゴケ / オルガネラ形成 / 脂質結合 / Peroxin / GFP / 進化 / 共通性／多様性 / 非モデル植物 / 形質転換植物 / RNA-seq / タンパク質間相互作用 / 適応性

Outline of Final Research Achievements

To better understand molecular mechanism(s) on diversity and adaptation of plant peroxisomes, we searched for peroxisomal genes in Marchantia polymorpha genome, which were homologous to Arabidopsis peroxisomal genes. The results using Marchantia polymorpha showed that PTS (Peroxisome Targeting Signal) 1 and PTS2 pathways, which were used in vascular land plants, were present in Marchantia polymorpha, indicating that these pathways were acquired at the earlier evolutionary history for land plants. In addition, we identified some Arabidopsis APEM (Aberrant PEroxisome Morphology) genes, whose defects affected peroxisome biogenesis including protein transport, and characterized their gene-products.
As a research tool, we generated various Gateway technology-compatible destination vectors that were dedicated for Marchantia polymorpha research. These vectors are provided to researchers at home and abroad free of cost.

Academic Significance and Societal Importance of the Research Achievements

ペルオキシソーム形成や機能発現の制御機構の解析は、これまでシロイヌナズナを中心に進められてきたが、このシロイヌナズナの知見が植物に共通なものなのかは明らかにされていなかった。本研究成果から、ペルオキシソームタンパク質の輸送機構については共通性が高いこと、進化の過程で遺伝子重複により機能の多様性を獲得していったことが明らかとなった。ペルオキシソームは、植物のみならず動物や酵母の細胞においても必須のオルガネラであることから、本研究成果は、真核生物におけるペルオキシソーム形成や機能発現の共通原理の理解における知見として意義がある。

Research Products

• [Journal Article] Regulation of sugar and storage oil metabolism by phytochrome during de-etiolation (2020)
  • Author(s): Kozuka, T., Sawada, Y., Imai, H., Kanai, M., Yokota-Hirai, M., Mano, S., Uemura, M., Nishimura, M., Kusaba, M., and Nagatani, A.
  • Journal Title: Plant Physiology Volume: 182 Issue: 2 Pages: 1114-1129
  • DOI: 10.1104/pp.19.00535
  • Peer Reviewed / Open Access
• [Journal Article] Sucrose starvation induces microautophagy in plant root cells (2019)
  • Author(s): Goto-Yamada, S., Oikawa, K., Bizan, J., Shigenobu, S., Yamaguchi, K., Mano, S., Hayashi, M., Ueda, H., Hara-Nishimura, I., Nishimura, M., and Yamada, K.
  • Journal Title: Frontiers in Plant Science Volume: 10 Pages: 1604-1604
  • DOI: 10.3389/fpls.2019.01604
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Autophagy controls reactive oxygen species homeostasis in guard cells that is essential for stomatal opening (2019)
  • Author(s): Yamauchi, S., Mano, S., Oikawa, K., Hikino, K., Teshima, M.K., Kimori, Y., Nishimura, M., Shimazaki, K., and Takemiya, A.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 116 Issue: 38 Pages: 19187-19192
  • DOI: 10.1073/pnas.1910886116
  • Peer Reviewed
• [Journal Article] Gateway binary vectors with organelle-targeted fluorescent proteins for highly sensitive reporter assay in gene expression analysis of plants. (2019)
  • Author(s): Sultana, M.M., Dutta, A.K., Tanaka, Y., Aboulela, M., Nishimura, K., Sugiura, S., Niwa, T., Maeo, K., Goto-Yamada, S., Kimura, T., Ishiguro, S., Mano, S., and Nakagawa, T.
  • Journal Title: Journal of Biotechnology Volume: 297 Pages: 19-27
  • DOI: 10.1016/j.jbiotec.2019.03.015
  • NAID: 120006845280
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Novel gateway binary vectors for rapid tripartite DNA assembly and promoter analysis with various reporters and tags in the liverwort Marchantia polymorpha. (2018)
  • Author(s): Mano, S., Nishihama, R., Ishida, S., Hikino, K., Kondo, M., Nishimura, M., Yamato, T.K., Kohchi, K., and Nakagawa, T.
  • Journal Title: PLOS ONE Volume: 3 Issue: 10 Pages: e0204964-e0204964
  • DOI: 10.1371/journal.pone.0204964
  • NAID: 120006534596
  • Peer Reviewed / Open Access
• [Journal Article] Effect of mutation of C-terminal and heme binding region of Arabidopsis catalase on the import to peroxisomes. (2018)
  • Author(s): Fujikawa, Y., Suekawa, M., Endo, S., Fukami, Y., Mano, S., Nishimura, M., and Esaka, M.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 83 Issue: 2 Pages: 322-325
  • DOI: 10.1080/09168451.2018.1530094
  • Peer Reviewed / Open Access
• [Journal Article] A dual-site gateway cloning system for simultaneous cloning of two genes for plant transformation. (2017)
  • Author(s): Aboulela, M., Tanaka, Y., Nishimura, K., Mano, S., Kimura, T., and Nakagawa, T.
  • Journal Title: Plasmid Volume: 92 Pages: 1-11
  • DOI: 10.1016/j.plasmid.2017.05.001
  • Peer Reviewed
• [Journal Article] Development of an R4 dual-site (R4DS) gateway cloning system enabling the efficient simultaneous cloning of two desired sets of promoters and open reading frames in a binary vector for plant research. (2017)
  • Author(s): Aboulela, M., Tanaka, Y., Nishimura, K., Mano, S., Nishimura, M., Ishiguro, S., Kimura, T., and Nakagawa, T.
  • Journal Title: PLoS One Volume: 12 Issue: 5 Pages: 177889-177901
  • DOI: 10.1371/journal.pone.0177889
  • Peer Reviewed / Open Access
• [Journal Article] An efficient method for the isolation of highly purified RNA from seeds for use in quantitative transcriptome analysis. (2017)
  • Author(s): Kanai, M., Mano, S., and Nishimura, M.
  • Journal Title: J. Vis. Exp. Volume: 119 Issue: 119
  • DOI: 10.3791/55008
  • Peer Reviewed
• [Presentation] ペルオキシソーム形成に関わるシロイヌナズナAPEM6様タンパク質の機能比較 (2021)
  • Author(s): 神垣あかね、西村幹夫、真野昌二
  • Organizer: 第62回日本植物生理学会年会
• [Presentation] 水溶性ビタミンを介した母体による胚と胚乳の発生制御 (2021)
  • Author(s): 石本聖絵、鈴木雅晴、杉木愛、木嵜暁子、真野昌二、野坂（髙橋）実鈴、鈴木俊哉、タキムニュン、志水（佐藤）佐江、豊田敦、鈴木孝征、田畑亮、櫻井望、澤進一郎、長戸康郎、マッカーティードナルド、佐藤豊
  • Organizer: 日本植物学会第84回大会
• [Presentation] オルガネラ形成に関わるシロイヌナズナAPEM6ホモログの機能解析 (2020)
  • Author(s): 神垣あかね、西村幹夫、真野昌二
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] オートファジーは孔辺細胞ROSホメオ スタシスを制御することで青色光依存の気孔開口を可能にする (2020)
  • Author(s): 山内翔太、真野昌二、及川和聡、曳野和美、手島康介、木森義隆、西村幹生、島崎研一郎、武宮淳史
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] ペルオキシソーム形成に関わる新規因子APEM6の機能と局在制御 (2019)
  • Author(s): 神垣あかね、西村幹夫、真野昌二
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] 植物細胞を覗く ～ミクロの世界の探索～ (2018)
  • Author(s): 真野昌二
  • Organizer: 大学共同利用機関シンポジウム2018 ～最先端研究大集合～
  • Invited
• [Presentation] dentification and characterization of molecular players for peroxisome biogenesis and functions based on imaging-based approach (2017)
  • Author(s): Mano, S., Goto-Yamada, S., Oikawa, K., and Nishimura, M.
  • Organizer: Taiwan-Japan Plant Biology 2017
  • Int'l Joint Research
• [Presentation] Development of two Gateway binary vector series for the assembly of three DNA fragments and promoter analysis in Marchantia polymorpha (2017)
  • Author(s): Mano, S., Nishihama, R., Ishida, S., Kazumi, H., Kondo, M., Yamato, K., Kohchi, T., and Nakagawa, T.
  • Organizer: The 65th NIBB Conference Marchantia Workshop 2017 Renaissance of Marchantia polymorpha -the genome and beyond
  • Int'l Joint Research
• [Presentation] イメージング技術を用いた植物ペルオキシソーム研究 (2017)
  • Author(s): 真野昌二
  • Organizer: 第1回プラントバイオイメージング研究会
  • Invited
• [Presentation] Roles of LON protease and autophagy in the peroxisome quality control. (2017)
  • Author(s): Goto-Yamada, S., Mano, S., Oikawa, K., and Nishimura, M.
  • Organizer: EUROBIOTEC 2017 EXPO
  • Int'l Joint Research
• [Presentation] 植物の高次機能を支えるペルオキシソーム機能発現と形成機構 (2017)
  • Author(s): 真野昌二、及川和聡、後藤-山田志野、Cui Songkui、林誠、西村幹夫
  • Organizer: ConBio2017
  • Invited
• [Book] Methods in Molecular Biology. Two-Hybrid Systems. Methods and Protocol (2018)
  • Author(s): Goto-Yamada, S., Hikino, K., Nishimura, M., Nakagawa, T., and Mano, S.
  • Total Pages: 355
  • Publisher: Springer.
  • ISBN: 9781493978700
• [Remarks] オルガネラ制御研究室
  • URL: http://www.nibb.ac.jp/plantorganelles/