Intertissue networks of water transpiration control in plants

• Project/Area Number: 17K07458
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Kuromori Takashi 国立研究開発法人理化学研究所, 環境資源科学研究センター, 上級研究員 (80332295)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 植物ホルモン / アブシジン酸 / 維管束 / 気孔 / 孔辺細胞 / 膜輸送 / 膜タンパク質 / 変異体 / 植物 / 遺伝子

Outline of Final Research Achievements

Abscisic acid (ABA) is a key phytohormone that prevents water loss from the plant body. We examined whether ABA has an endocrine-type action or an autocrine-type action, by using molecular biological approaches. Consequently, I found that ABA looked to be able to function by both actions of endocrine and autocrine, which probably would be a unique strategy in plant survival.

Academic Significance and Societal Importance of the Research Achievements

多細胞生物において、ホルモンなどの生理活性物質は、生合成場所から作用場所への機敏で正確な伝播制御が要求されます。植物にとって水分状態を感知・伝播して、気孔を最適な状態に維持していくことは、個体レベルで水分蒸散を調節するための最も重要なネットワーク制御の一つです。水分蒸散を制御する植物ホルモンの生合成と組織間における伝播輸送ネットワーク、そしてそこに関わる制御因子と作用機序の理解を目指しています。

Research Products

• [Journal Article] Evidence for potassium transport activity of Arabidopsis KEA1-KEA6. (2019)
  • Author(s): Masaru Tsujii, Kota Kera, Shin Hamamoto, Takashi Kuromori, Toshiharu Shikanai, Nobuyuki Uozumi
  • Journal Title: Scientific Report Volume: 9 (1), 10040 Issue: 1 Pages: 10040-10040
  • DOI: 10.1038/s41598-019-46463-7
  • Peer Reviewed / Open Access
• [Journal Article] SnRK1 kinase and the NAC transcription factor SOG1 are components of a novel signaling pathway mediating the low energy response triggered by ATP depletion. (2019)
  • Author(s): Hamasaki H, Kurihara Y, Kuromori T, Kusano H, Nagata N, Yamamoto YY, Shimada H, Matsui M
  • Journal Title: Frontiers in Plant Science Volume: 10 Pages: 503-503
  • DOI: 10.3389/fpls.2019.00503
  • Peer Reviewed / Open Access
• [Journal Article] ABA transport and plant water stress responses. (2018)
  • Author(s): Kuromori T, Seo M, Shinozaki K
  • Journal Title: Trends in Plant Science Volume: 23 Issue: 6 Pages: 513-522
  • DOI: 10.1016/j.tplants.2018.04.001
  • Peer Reviewed
• [Journal Article] Regulatory Gene Networks in Drought Stress Responses and Resistance in Plants. (2018)
  • Author(s): Takahashi F, Kuromori T, Sato H, Shinozaki K
  • Journal Title: Advances in Experimental Medicine and Biology Volume: 1081 Pages: 189-214
  • DOI: 10.1007/978-981-13-1244-1_11
  • ISBN: 9789811312434, 9789811312441
  • Peer Reviewed
• [Journal Article] Functional relationship of AtABCG21 and AtABCG22 in stomatal regulation (2017)
  • Author(s): Kuromori T, Sugimoto E, Ohiraki H, Yamaguchi-Shinozaki K, Shinozaki K
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 12501-12501
  • DOI: 10.1038/s41598-017-12643-6
  • Peer Reviewed / Open Access
• [Journal Article] Acetate-mediated novel survival strategy against drought in plants (2017)
  • Author(s): Kim JM, To TK, Matsui A, Tanoi K, Kobayashi NI, Matsuda F, Habu Y, Ogawa D, Sakamoto T, Matsunaga S, Bashir K, Rasheed S, Ando M, Takeda H, Kawaura K, Kusano M, Fukushima A, Endo TA, Kuromori T, Ishida J, Morosawa T, Tanaka M, Torii C, Takebayashi Y, Sakakibara H, Ogihara Y, Saito K, Shinozaki K, Devoto A, Seki M
  • Journal Title: Nature Plants Volume: 3 Issue: 7 Pages: 17097-17097
  • DOI: 10.1038/nplants.2017.97
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 生理活性物質の膜輸送に関わるトランスポーターの機能解析 (2020)
  • Author(s): 黒森 崇、杉本 絵理子、篠崎 一雄
  • Organizer: 第67回応用物理学会春季学術講演会
  • Invited
• [Presentation] Functional complementation of guard cell specific or phloem companion cell specific ABA biosynthesis. (2020)
  • Author(s): Kuromori T, Fujita M, Sugimoto E, Kikuchi S, Shinozaki K
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] Extraction of RNA from an intact single plant cell with cell wall using focused electric field. (2019)
  • Author(s): Parimalam SS, Sotta N, Kuromori T, Fujiwara T, Yokota-Hirai M, Shintaku H
  • Organizer: 30th International Symposium on Micro-NanoMechatronics and Human Science
  • Int'l Joint Research
• [Presentation] 植物トランスポーターの膜輸送アッセイによる機能解析 (2019)
  • Author(s): 黒森 崇、杉本 絵理子、篠崎 一雄
  • Organizer: 第56回アイソトープ・放射線研究発表会
• [Presentation] Functional screening of membrane transporters for a specific substance (2019)
  • Author(s): Kuromori T, Sugimoto E, Shinozaki K
  • Organizer: 第92回日本生化学会大会
• [Presentation] Functional complementation of ABA biosynthesis using cell-type specific promoters (2019)
  • Author(s): Kuromori T, Sugimoto E, Shinozaki K
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] 生理活性物質の膜輸送に関わるトランスポーターの遺伝子機能解析 (2018)
  • Author(s): 黒森 崇、杉本 絵理子、篠崎 一雄
  • Organizer: 第91回日本生化学会大会
  • Invited
• [Presentation] Is ABA an endocrine-type hormone or an autocrine-type hormone? (2018)
  • Author(s): Kuromori T, Sugimoto E, Shinozaki K
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] 生理活性物質を輸送するトランスポーターの機能的スクリーニング法の開発 (2017)
  • Author(s): 黒森 崇、杉本 絵理子、篠崎 一雄
  • Organizer: 第12回トランスポーター研究会年会
• [Presentation] 大腸菌を宿主細胞としてシロイヌナズナK+/H+アンチポーターの機能解析 (2017)
  • Author(s): 辻井 雅、解良 康太、浜本 晋、黒森 崇、鹿内 利治、魚住 信之
  • Organizer: 第12回トランスポーター研究会年会
• [Presentation] Funcional identification of K+/H+ antiporter involved in Non photochemical quenching in Arabidopsis thaliana. (2017)
  • Author(s): Tsujii M, Kuromori T, Shikanai T, Uozumi N
  • Organizer: 第69回日本生物工学会大会
• [Remarks] 研究紹介
  • URL: http://genediscovery.riken.jp/kuromori.html