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Molecular regulation of actin plate on brown algal cytokinesis

Research Project

Project/Area Number 17K07462
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionHokkaido University

Principal Investigator

NAGASATO Chikako  北海道大学, 北方生物圏フィールド科学センター, 准教授 (00374710)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords細胞質分裂 / 褐藻類 / アクチン / 微小管 / 植物
Outline of Final Research Achievements

In this project, the brown alga, Halopteris congesta (Sphacelariales) with an apical meristem cell was used to study function of actin plate unique cytokinetic apparatus in brown algae. To achieve this object, live imaging of microtubules (MTs) and actins, pharmacological experiments using cytokinesis inhibitors, and isolation of proteins relating progress of cytokinesis were conducted. The results gave me insight for actin plate as follows: involvement of MTs in the formation and maintain of actin plate, and function of actin plate for the formation of a new cytokinetic plane.

Academic Significance and Societal Importance of the Research Achievements

これまで、細胞質分裂における知見は、動物・酵母(オピストコンタ)、陸上植物(アーケプラスチダ)から得られたものであり、真核生物全体における細胞質分裂の共通性と多様性を理解するためには、褐藻類といった他の真核生物のグループからのアプローチも必要であるという点で学術的意義がある。また、細胞質分裂が正しく行われるかどうかは、その後の多細胞体制の構築、発生に重要な意味をもたらす。沿岸生態系を支える褐藻類の体の作り方を理解することは社会的意義のあるものだと考える。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (1 results)

  • [Int'l Joint Research] CNRS UPMC Station Biologique Roscoff(フランス)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] CNRS UPMC Station Biologique Roscoff(フランス)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Effect of brefeldin A and the dynamics of the actin plate on cytokinesis of zygotes in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae)2018

    • Author(s)
      Nagasato Chikako、Motomura Taizo
    • Journal Title

      European Journal of Phycology

      Volume: 54 Issue: 1 Pages: 26-38

    • DOI

      10.1080/09670262.2018.1501516

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] DISTAG/TBCCd1 Is Required for Basal Cell Fate Determination inEctocarpus2017

    • Author(s)
      Godfroy Olivier、Uji Toshiki、Nagasato Chikako、Lipinska Agnieszka P.、Scornet Delphine、Peters Akira F.、Avia Komlan、Colin Sebastien、Mignerot Laure、Motomura Taizo、Cock J. Mark、Coelho Susana M.
    • Journal Title

      The Plant Cell

      Volume: 29 Issue: 12 Pages: 3102-3122

    • DOI

      10.1105/tpc.17.00440

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 褐藻類の細胞質分裂時に出現するアクチンと隔膜形成,微小管との関係について2020

    • Author(s)
      青木日向子・本村泰三・長里千香子
    • Organizer
      日本藻類学会第44回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 褐藻類の細胞質分裂におけるアクチンの挙動2019

    • Author(s)
      青木日向子・本村泰三・長里千香子
    • Organizer
      日本植物形態学会第31回
    • Related Report
      2019 Annual Research Report
  • [Presentation] Dynamics of actin plate on brown algal cytokinesis2017

    • Author(s)
      Nagasato, C., Motomura, T.
    • Organizer
      The 8th Asian Pacific Phycological Forum
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 褐藻類の細胞質分裂についての微細構造学的解析2017

    • Author(s)
      長里千香子
    • Organizer
      第73回日本顕微鏡学会学術講演会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Book] Cryofixation of brown algae for Transmission Electron Microscopy. In Protocols for Macroalgae Research2018

    • Author(s)
      C. Nagasato, C. Katsaros, T. Motomura
    • Publisher
      Taylor & Francis Group
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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