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Jellyfish oocyte maturation triggered by light or dark stimulus

Research Project

Project/Area Number 17K07482
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Animal physiology/Animal behavior
Research InstitutionMiyagi University of Education

Principal Investigator

Deguchi Ryusaku  宮城教育大学, 大学院教育学研究科高度教職実践専攻, 教授 (90302257)

Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords卵母細胞 / 放卵 / 神経ペプチド / 生殖隔離 / 放精 / 刺胞動物 / ヒドロ虫綱 / 卵成熟誘起ホルモン / 生殖的隔離 / エダアシクラゲ / 光刺激 / オプシン / 光受容 / 配偶子放出 / 卵成熟 / 動物 / 生殖 / 配偶子
Outline of Final Research Achievements

There exist light (L)-type and dark (D)-type medusae, in which oocyte maturation and spawning occur in response to light stimulation (light after dark) and dark stimulation (dark after light), respectively, in the hydrozoan jellyfish Cladonema pacificum. The present study provides evidence that RPRPamide or related peptides released from the gonad epithelium functions as maturation-inducing hormone in both types. The F1 hybrids obtained from heterotypic crossings could spawn in response to both light and dark stimulations, although stronger changes in light intensity were necessary for the spawning of F1 hybrids. Interestingly, the F2 generation that inherits either or both of F1 hybrid chromosome sets failed to metamorphose into polyps. These data, together with the results of molecular phylogenetic analysis using DNA sequences, support the idea that reproductive isolation has been established between L- and D-type C. pacificum, which might be regarded as separate species now.

Academic Significance and Societal Importance of the Research Achievements

原始的な動物であるクラゲでは、光環境の変化に応じて卵成熟およびそれに続く放卵が起こることが知られていたが、真逆の刺激(明刺激と暗刺激)に反応するクラゲの比較解析が行われたのは本研究が初めてである。得られた結果は新規性が高く、動物における卵成熟機構の「進化」や「原型」の理解を促すものである。また、全国的に人気のある水族館でのクラゲ展示などにも影響を与える重要な成果であると考えている。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (30 results)

All 2022 2021 2020 2019 2018 2017

All Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (19 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Book (2 results)

  • [Journal Article] Gene expression alterations from reversible to irreversible stages during coral metamorphosis2022

    • Author(s)
      Yuu Ishii, Masayuki Hatta, Ryusaku Deguchi, Masakado Kawata, Shinichiro Maruyama
    • Journal Title

      Zoological Letters

      Volume: 8 Issue: 1 Pages: 4-4

    • DOI

      10.1186/s40851-022-00187-1

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 宮城県沿岸で採集されたエダクダクラゲ類の特徴2021

    • Author(s)
      菊地充, 斉藤千映美, 出口竜作
    • Journal Title

      宮城教育大学 環境教育研究紀要

      Volume: 23 Pages: 79-84

    • NAID

      120007147289

    • Related Report
      2021 Annual Research Report
    • Open Access
  • [Journal Article] Isolation and Characterization of Feeding-Deficient Strains in Inbred Lines of the Hydrozoan Jellyfish Cladonema pacificum2020

    • Author(s)
      Tachibana Kazunori, Matsumoto Masaaki, Minowa Aiko, Deguchi Ryusaku
    • Journal Title

      Zoological Science

      Volume: 37 Issue: 3 Pages: 263-270

    • DOI

      10.2108/zs190122

    • NAID

      40022252843

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] クラゲの繁殖戦略─無性生殖から有性生殖, 単為生殖まで2020

    • Author(s)
      出口竜作、菅原朱莉
    • Journal Title

      生物の科学 遺伝

      Volume: 74 Pages: 394-401

    • Related Report
      2020 Research-status Report
  • [Journal Article] クラゲの卵成熟誘起ホルモンと光受容によるホルモン放出2019

    • Author(s)
      竹田典代, Evelyn Houliston, 出口竜作
    • Journal Title

      Comparative Endocrinology

      Volume: 45 Issue: 168 Pages: 111-115

    • DOI

      10.5983/nl2008jsce.45.111

    • NAID

      130007729095

    • ISSN
      1882-6636, 1882-6644
    • Related Report
      2019 Research-status Report
    • Open Access / Int'l Joint Research
  • [Journal Article] Reproductive Strategies in Marine Hydrozoan Jellyfish: Sexual Medusae and Asexual Polyps2018

    • Author(s)
      Takeda, N., Deguchi, R., Itabashi, T.
    • Journal Title

      Reproductive and Developmental Strategies: The Continuity of Life

      Volume: - Pages: 157-174

    • DOI

      10.1007/978-4-431-56609-0_8

    • ISBN
      9784431566076, 9784431566090
    • Related Report
      2018 Research-status Report
  • [Journal Article] Fertilization, Comparative2018

    • Author(s)
      Deguchi, R., Hirohashi, N.
    • Journal Title

      Encyclopedia of Reproduction, 2nd edition

      Volume: 6 Pages: 344-349

    • DOI

      10.1016/b978-0-12-809633-8.20576-1

    • ISBN
      9780128151457
    • Related Report
      2018 Research-status Report
  • [Journal Article] A gonad-expressed opsin mediates light-induced spawning in the jellyfish Clytia2018

    • Author(s)
      Quiroga Artigas, G., Lapebie, P., Leclere, L., Takeda, N., Deguchi, R., Jekely, G., Momose, T., Houliston, E.
    • Journal Title

      eLife

      Volume: 7

    • DOI

      10.7554/elife.29555

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of jellyfish neuropeptides that act directly as oocyte maturation-inducing hormones2018

    • Author(s)
      Takeda, N., Kon, Y., Quiroga Artigas, G., Lapebie, P., Barreau, C., Koizumi, O., Kishimoto, T., Tachibana, K., Houliston, E., Deguchi, R.
    • Journal Title

      Development

      Volume: 145 Issue: 2

    • DOI

      10.1242/dev.156786

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] エダクダクラゲのクラゲ形成条件2021

    • Author(s)
      菊地充, 出口竜作
    • Organizer
      第92回日本動物学会オンライン米子大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] エダアシクラゲのオスクラゲに見られる口側から波状に広がる放精2021

    • Author(s)
      竹田典代, 出口竜作, 彦坂暁
    • Organizer
      第92回日本動物学会オンライン米子大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] サンゴ発生過程における変態可逆性に関わる遺伝子発現パターン2021

    • Author(s)
      石井悠, 服田昌之, 出口竜作, 河田雅圭, 丸山真一朗
    • Organizer
      第92回日本動物学会オンライン米子大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 褐虫藻が低pH条件でグルコースを分泌するメカニズムと意義2021

    • Author(s)
      石井悠、石井宏憲、高橋俊一、黒羽剛、篠原直貴、横山隆亮、出口竜作、西谷和彦、皆川純、河田雅圭、丸山真一朗
    • Organizer
      日本藻類学会第45回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 宮城県沿岸のミサキコモチエダクダクラゲとエダクダクラゲの比較解析2020

    • Author(s)
      菊地充、出口竜作
    • Organizer
      日本動物学会2020年度東北支部大会
    • Related Report
      2020 Research-status Report
  • [Presentation] カイヤドリヒラムシの成長に及ぼす給餌頻度と水温の影響2020

    • Author(s)
      髙橋真湖、出口竜作
    • Organizer
      日本動物学会2020年度東北支部大会
    • Related Report
      2020 Research-status Report
  • [Presentation] エダアシクラゲの暗タイプにおける自然光下での放卵のタイミング2019

    • Author(s)
      菅原悠香, 山川奈帆美, 出口竜作
    • Organizer
      日本動物学会令和元年度東北支部大会
    • Related Report
      2019 Research-status Report
  • [Presentation] エダアシクラゲの暗タイプの放卵を誘起する光条件と卵成熟誘起神経ペプチドの動態2019

    • Author(s)
      菅原悠香, 出口竜作
    • Organizer
      日本動物学会第90回大阪大会
    • Related Report
      2019 Research-status Report
  • [Presentation] エダアシクラゲの摂食障害変異体の単離と解析2019

    • Author(s)
      立花和則, 松本眞明, 箕輪愛子, 出口竜作
    • Organizer
      日本動物学会第90回大阪大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Oocyte maturation and spawning of light- and dark-type medusae of the hydrozoan Cladonema pacificum2019

    • Author(s)
      Deguchi, R., Hoshi, T., Takeda, N., Tachibana, K.
    • Organizer
      Fifth International Oocyte Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular phylogenetic analysis and hybridization in light-type and dark-type Cladonema pacificum2019

    • Author(s)
      Hoshi, T., Nishizumi, K., Deguchi, R., Tachibana, K.
    • Organizer
      Fifth International Oocyte Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Feeding behaviour of the jellyfish Cladonema2018

    • Author(s)
      Thoma, V., Kobayashi, K., Suzuki, M., Deguchi, R., Tanimoto, H.
    • Organizer
      Neuro-Evo: A Comparative Approach to Cracking Circuit Function II
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] エダアシクラゲの放卵を誘起する光条件2018

    • Author(s)
      菅原悠香, 石森彩希, 山川奈帆美, 星尚仁, 出口竜作
    • Organizer
      日本動物学会平成30年度東北支部大会
    • Related Report
      2018 Research-status Report
  • [Presentation] エダアシクラゲにおける分子系統解析の妥当性─表現型とミトコンドリアDNA相同性の不一致2018

    • Author(s)
      星尚仁, 西住恵太, 出口竜作, 立花和則
    • Organizer
      日本動物学会第89回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 浅虫はエダアシクラゲの明タイプと暗タイプのボーダーか?2017

    • Author(s)
      星尚仁、出口竜作、立花和則
    • Organizer
      日本動物学会平成29年度東北支部大会
    • Related Report
      2017 Research-status Report
  • [Presentation] クラゲの有性化のための環境要因2017

    • Author(s)
      菅原朱莉、出口竜作
    • Organizer
      日本動物学会第88回富山大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] クラゲの卵成熟誘起ホルモン2017

    • Author(s)
      出口竜作、竹田典代
    • Organizer
      日本動物学会第88回富山大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 配偶子放出条件の異なるエダアシクラゲの掛け合わせによる分類の再検討2017

    • Author(s)
      星尚仁、西住恵太、立花和則、出口竜作
    • Organizer
      日本動物学会第88回富山大会
    • Related Report
      2017 Research-status Report
  • [Presentation] エダアシクラゲの配偶子放出の光制御2017

    • Author(s)
      林卓央、亀井保博、出口竜作、立花和則
    • Organizer
      日本動物学会第88回富山大会
    • Related Report
      2017 Research-status Report
  • [Book] Reproduction in Aquatic Animals2020

    • Author(s)
      Manabu Yoshida, Juan F. Asturiano, Ryusaku Deguchi, Makoto Osada ほか
    • Total Pages
      379
    • Publisher
      Springer
    • ISBN
      9789811522901
    • Related Report
      2020 Research-status Report
  • [Book] Developmental Biology of the Sea Urchin and Other Marine Invertebrates2020

    • Author(s)
      David J. Carroll, Stephen A. Stricker, Ryusaku Deguchi, Takeshi Nakano, ほか
    • Total Pages
      309
    • Publisher
      Humana Press
    • ISBN
      9781071609736
    • Related Report
      2020 Research-status Report

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Published: 2017-04-28   Modified: 2023-01-30  

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