| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We demonstrated that KNL2, a member of the Mis18 complex, was essential for the stable inheritance of the centromere position at G1-phase through direct binding to CENP-A nucleosome to deposit new CENP-A in chicken DT40 cells. We revealed the 3D architecture of the interphase genome, including the centromeres by systematic 4C-seq analysis using DT40 cell lines. Based on structural and genetic analyses, we conclude that a CENP-A-mediated structural polymorphism facilitated the preferential H4K20 monomethylation in centromeric nucleosomes. These findings may provide insight into developing an anti-cancer drug utilizing chromatin modulation.
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