| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
In this study, we introduced frameshift mutations into the medaka cone opsin genes (eight subtypes in total) using the CRISPR/Cas9 system. Quantitation of behavioral photosensitivity based on the optomotor response under monochromatic light showed that mutants lacking red opsin had reduced red light sensitivity, whereas mutants lacking violet opsin had similar UV sensitivity to that of wild type. In addition, medaka that lost two types of opsins, violet and blue, also sensed UV light. These results are interesting in that they suggest that the responses of animals to ultraviolet light are not necessarily mediated by violet or blue opsins, and that green and red opsins alone are capable of responding to a wide range of wavelengths. Continued analysis of these mutants will advance our understanding of animal color vision.
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