| Project/Area Number |
17K07517
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Evolutionary biology
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
KANAI Akio 慶應義塾大学, 環境情報学部(藤沢), 教授 (60260329)
|
|---|
| Project Period (FY) |
2017-04-01 – 2020-03-31
|
| Project Status |
Completed (Fiscal Year 2019)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 分子進化 / ポリヌクレオチドリン酸化酵素 / 情報学的解析 / 実験検証 / 巨大タンパク質 / リン酸化酵素 / 新規巨大タンパク質 / 3大生物ドメイン / 生命情報科学 / タンパク質ドメイン / RNAキナーゼ / 系統樹 / 機能ドメイン / Clp1 / 進化情報 / マルチドメインタンパク質 / 基質特異性 / 合成生物学 / CLP1 |
|---|
| Outline of Final Research Achievements |
We conducted a large-scale molecular evolutionary analysis of the Clp1 family proteins, a polyribonucleotide 5'-hydroxyl kinases. In total, 3,557 Clp1 family proteins were detected in the three domains of life, Bacteria, Archaea, and Eukarya. Many were from Archaea and Eukarya, but a few were found in restricted, phylogenetically diverse bacterial species. The domain structures of the Clp1 family proteins also differed among the three domains of life. Although the proteins were, on average, 555 amino acids long (range, 196-2,728), 122 large proteins with > 1,000 amino acids were detected in eukaryotes. The polyribonucleotide kinase activity of Thermus scotoductus Clp1 was characterized experimentally. We propose a comprehensive assessment of the diversification of the Clp1 family proteins and the molecular evolution of their functional domains.
|
| Academic Significance and Societal Importance of the Research Achievements |
RNAキナーゼであるClp1に関して、タンパク質のドメインレベルで、特に真核生物において機能の分担をしながら分子進化していく様相を明らかにできた。この研究過程で、Clp1のキナーゼドメインを有した巨大タンパク質 (Clp1様タンパク質)を見出した。Clp1はヒトで遺伝病の原因遺伝子となっていることから、今回見出したClp1様タンパク質も遺伝病に関わる可能性がある。また、バクテリアで初めてとなるClp1分子を同定し、その生化学的な活性に関して検証した。
|
