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Taxonomic study and an iron-corroding mechanism of iron-corroding and nitrate-reducing bacteria

Research Project

Project/Area Number 17K07546
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biodiversity/Systematics
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Iino Takao  国立研究開発法人理化学研究所, バイオリソース研究センター, 専任研究員 (50550323)

Project Period (FY) 2017-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Keywords微生物腐食 / 鉄酸化 / 硝酸還元 / 天然ガス回収施設 / Mariniphaga / Prolixibacter / 鉄腐食 / 硝酸塩還元菌 / 金属腐食 / 微生物 / 分類学
Outline of Final Research Achievements

Microbiologically influenced corrosion (MIC) of metallic materials imposes a heavy economic burden. Eleven novel bacterial strains were isolated from brine of a natural gas recovery facility in Japan. Among eleven strains, strain NT050 oxidized the iron (Fe0) foil in the presence of nitrate and yeast extract. On the other hand, strain NT017 was related to nitrate-reducing and iron-oxidizing Prolixibacter denitrificans MIC1-1 based on the 16S rRNA gene sequence similarity, while did not oxidized Fe0 foil under any culture condition In this study, a novel iron-corroding bacterium was obtained by culture-depend technique. Furthermore, Prolixibacter contained both Fe0-corroding bacteria and Fe0-non-corroding bacteria.

Academic Significance and Societal Importance of the Research Achievements

高効率エネルギー社会を構築する上で、重大な経済損失や環境問題を招く微生物腐食は、予測困難なため有効な対策技術がなく、問題が深刻化している。長らく、硫酸塩還元菌が微生物腐食の主要な原因菌として捉えられてきたが、その実態は未解明な点が多く、未だ有効な防食方法がない。金属腐食菌の正確な種の特定や自然環境中の微生物生態系の解明なしに、防食対策を講じることは不可能である。金属腐食菌の培養株の充実は、微生物腐食を未然に予測する診断技術の開発するための貴重な微生物材料となり、高効率エネルギー社会構築の実現に向け、その意義は高い。

Report

(7 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (5 results)

All 2022 2021 2020 2019

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Nitrite as a causal factor for nitrate‐dependent anaerobic corrosion of metallic iron induced by Prolixibacter strains2021

    • Author(s)
      Iino Takao、Shono Nobuaki、Ito Kimio、Nakamura Ryuhei、Sueoka Kazuo、Harayama Shigeaki、Ohkuma Moriya
    • Journal Title

      MicrobiologyOpen

      Volume: 10 Issue: 4

    • DOI

      10.1002/mbo3.1225

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Prolixibacter 属細菌2 新種による硝酸からの亜硝酸生成による金属鉄腐食2022

    • Author(s)
      飯野隆夫、大熊盛也
    • Organizer
      一般社団法人 日本鉄鋼協会
    • Related Report
      2021 Research-status Report
  • [Presentation] 鉄腐食性Prolixibacter属細菌のゲノム解析2020

    • Author(s)
      飯野隆夫
    • Organizer
      日本鉄鋼協会2020年秋季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 天然ガス回収施設由来の鉄スケールおよび鹹水に生息する鉄腐食硝酸塩還元菌の探索2019

    • Author(s)
      飯野隆夫、伊藤公夫、大熊盛也
    • Organizer
      日本微生物資源学会第26回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Quantitative analysis of Prolixibacter, which accommodating with iron-corroding nitrate-reducing bacteria, in microbial mats, brines and seawater.2019

    • Author(s)
      Takao Iino, Kimio Ito, Chol Gyu Lee, Toshiyuki Sunaba and Moriya Ohkuma
    • Organizer
      EUROCORR 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2024-01-30  

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