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Do legumes use nitrogen luxuriously?: nitrogen resorption from senescing leaf

Research Project

Project/Area Number 17K07554
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ecology/Environment
Research InstitutionIbaraki University

Principal Investigator

OIKAWA SHIMPEI  茨城大学, 理工学研究科(理学野), 准教授 (90400308)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsマメ科植物 / 生物的窒素固定 / 窒素利用 / 窒素回収効率 / 構造タンパク質 / 代謝タンパク質 / マメ科 / 根粒 / 栽培化 / 系統関係 / 窒素固定 / 窒素回収 / タンパク質 / 種間比較 / 植物 / 生態学 / 環境 / 進化
Outline of Final Research Achievements

I studied nitrogen (N) resorption from senescing leaves of 25 legumes and 25 nonlegumes in a temperate region of Japan to test the hypothesis that high N resorption has not evolved in legumes that fix atmospheric N2. Senesced leaf N concentration was higher in legumes than in nonlegumes. The higher senesced leaf N concentration of legumes was associated with a lower proportion of leaf N resorbed during senescence, particularly in species with higher leaf N concentrations. I also found that legumes retained more metabolic proteins and structural proteins in their senesced leaves than nonlegumes, but the determination mechanism was different between metabolic- and structural proteins.

Academic Significance and Societal Importance of the Research Achievements

「窒素固定するマメ科植物は、老化中の葉から窒素を回収する能力が低く、リターには多くの窒素が多く残る」という考えは半ば通説化していた。しかし、これまで研究の対象となった種は少なく、系統だった解析は皆無であった。本研究課題では、野外調査と適切な統計的手法を用いてこの通説の真偽を明らかにした。Hierarchical partitioning解析を用いて枯葉の窒素濃度の変異に対する各要因の相対的貢献度を調べたところ、種の貢献度が最も高く、グループの貢献度は小さかった。これは、窒素固定の有無が窒素回収の主要な決定要因でないことを示唆する。このように、新たに窒素固定の有無以外の要因の重要性も示唆された。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (6 results)

All 2020 2019 2018 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Species-specific nitrogen resorption proficiency in legumes and nonlegumes2020

    • Author(s)
      Shimpei Oikawa; Yusuke Matsui; Michio Oguro; Masanori Okanishi; Ryo Tanabe; Tomoki Tanaka; Ayaka Togashi; Tomoyuki Itagaki
    • Journal Title

      Journal of Plant Research

      Volume: 133 Issue: 5 Pages: 639-648

    • DOI

      10.1007/s10265-020-01211-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Leaf shedding increases the photosynthetic rate of the canopy in N2-fixing and non-N2-fixing woody species2018

    • Author(s)
      Tomoki Tanaka, Chiharu Kurokawa, Shimpei Oikawa
    • Journal Title

      Tree Physiology

      Volume: 38 Issue: 12 Pages: 1903-1911

    • DOI

      10.1093/treephys/tpy104

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] 落葉は葉群光合成を最適化した: 3 年間の野外調査より2019

    • Author(s)
      田中 智紀, 黒川 千晴, 及川 真平
    • Organizer
      第66回日本生態学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 葉のタンパク質組成は窒素回収能力を決定するか:マメ科植物と非マメ科植物の比較2019

    • Author(s)
      田邊 亮, 及川 真平
    • Organizer
      第66回日本生態学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 栽培化に伴う葉の形質の変化:祖先種ツルマメとダイズ品種の比較2019

    • Author(s)
      冨樫 絢夏, 及川 真平
    • Organizer
      第66回日本生態学会大会
    • Related Report
      2018 Research-status Report
  • [Remarks] 研究内容

    • URL

      http://plantecology.sci.ibaraki.ac.jp/oikawa/workJ.html

    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2022-01-27  

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