Reproductive interference in geographic cline of corolla tube length in Isodon umbrosus

• Project/Area Number: 17K07559
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ecology/Environment
• Research Institution: Tokyo Gakugei University
• Principal Investigator: Dohzono Ikumi 東京学芸大学, 教育学部, 准教授 (70462489)
• Co-Investigator(Kenkyū-buntansha): 牧 雅之 東北大学, 学術資源研究公開センター, 教授 (60263985)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 地理的クライン / 繁殖干渉 / 形質置換 / 送粉生態学 / マルハナバチ / 送粉 / 地理的変異

Outline of Final Research Achievements

We investigated whether reproductive interference between different species resulted in the geographic cline of corolla length of Isodon umbrosus. Isodon trichocarpus with very short corolla coexists with I. umbrosus with short corolla, and they shared a common bumblebee pollinator, Bombus honshuensis. In sympatric site, fertilization of I. umbrosus was reduced by pollen deposition from I. trichocarpus, suggesting reproductive interference between species. Precedence of conspecific pollen was detected in sympatric populations in I. trichocarpus, suggesting character displacement in pollen-pistil interactions. In addition, we found two genetic groups in I. umbrosus, suggesting that a historical process, the secondary contact between two diverged populations, caused geographic cline of corolla length.

Academic Significance and Societal Importance of the Research Achievements

生物形質に地理的なクラインがみられることはよく知られており，気候適応の例は多いが，種間相互作用が要因となっている例は極めて少ない。結果，種間相互作用と遺伝的に分化したグループの２次的接触によって地理的クラインがもたらされた。異種との共存メカニズムが明らかになり，生物多様性の理解に貢献するものである。

Research Products

• [Journal Article] Frequent chloroplast capture among Isodon (Lamiaceae) species in Japan revealed by phylogenies based on variation in chloroplast and nuclear DNA (2019)
  • Author(s): Ogishima Miho、Horie Sachikio、Kimura Takuma、Yamashiro Tadashi、Dohzono Ikumi、Kawaguchi Lina、Nagano Atsushi J.、Maki Masayuki
  • Journal Title: Plant Species Biology Volume: 34 Issue: 3 Pages: 127-137
  • DOI: 10.1111/1442-1984.12239
  • Peer Reviewed
• [Journal Article] Hybridization, coexistence, and possible reproductive interference between native Oxalis corniculata and alien O. dillenii in Japan (2018)
  • Author(s): Fukatsu Misaki、Horie Sachiko、Maki Masayuki、Dohzono Ikumi
  • Journal Title: Plant Systematics and Evolution Volume: 305 Issue: 2 Pages: 127-137
  • DOI: 10.1007/s00606-018-1557-5
  • Peer Reviewed
• [Presentation] 同所的に生育する近縁植物2種の生殖隔離と繁殖干渉 (2019)
  • Author(s): 堂囿いくみ・荻嶋美帆・堀江佐知子・山城考・山田孝幸・牧雅之
  • Organizer: 日本生態学会第66回
• [Presentation] 同所的に生育する在来種カタバミ・外来種・雑種間の繁殖干渉 (2019)
  • Author(s): 堂囿いくみ*・深津美佐紀・加藤満里子・堀江佐知子・牧雅之
  • Organizer: 第51回種生物学シンポジウム
• [Presentation] 同所的に生育する近縁植物2種の生殖隔離と繁殖干渉 (2019)
  • Author(s): 堂囿いくみ，荻嶋美帆，堀江佐知子，山城考，山田孝幸，牧雅之
  • Organizer: 日本生態学会第66回大会
• [Presentation] 同所的に生育する近縁植物2種間の生殖隔離と集団間の種子生産比較 (2017)
  • Author(s): 中田大暁，堂囿いくみ
  • Organizer: 第49回種生物学シンポジウム
• [Remarks] IKUMI DOHZONO Web Page
  • URL: http://www.u-gakugei.ac.jp/~dohzono/index.html