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Development of the plant transformation vector harboring an auto-embryogenesis system

Research Project

Project/Area Number 17K07600
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Science in genetics and breeding
Research InstitutionChiba University

Principal Investigator

Igawa Tomoko  千葉大学, 大学院園芸学研究科, 准教授 (00360488)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords植物バイオテクノロジー / 遺伝子組換え / 分化 / 胚発生 / 脱分化 / 再分化 / 植物体再生 / 胚発生関連遺伝子 / 植物遺伝子組換え / 視覚マーカー
Outline of Final Research Achievements

To produce a genetically modified plant, gene transfer processes followed by plant regeneration from the transgenic cells are required. Plant regeneration from somatic cells often requires treatment with multiple types of plant hormones. However, exploring the conditions takes labor and time, hindering the development of plant science. In this study, we developed a vector system where embryogenesis-regulating genes in addition to the gene of interest are located. As a result, we succeeded in producing a gene transfer vector in which transgenic cells spontaneously differentiate without any plant hormone treatment.

Academic Significance and Societal Importance of the Research Achievements

遺伝子組換え技術は実用面だけでなく、様々な植物種での遺伝子機能解析を可能にし、有用な遺伝資源を見出すためにも必要な技術である。しかし、遺伝子組換え体作出法が適用できる植物種は限らるのが現状であり、それが細胞からの植物体再生法確立の難しさに起因している。本研究では組換え細胞が自発的に体作りを行う能力を目的遺伝子の導入と共に付与するシステムを構築しているため、今後多様な植物種での適用が見込まれる。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (5 results)

All 2020 2019 2017

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (3 results)

  • [Journal Article] Efficient plant regeneration and Agrobacterium-mediated transformation of Begonia semperflorens-cultorum2020

    • Author(s)
      Hirutani Sakiko、Shimomae Kazuki、Yaguchi Akira、Chin Dong Poh、Mii Masahiro、Igawa Tomoko
    • Journal Title

      Plant Cell, Tissue and Organ Culture (PCTOC)

      Volume: 142 Issue: 2 Pages: 435-440

    • DOI

      10.1007/s11240-020-01858-7

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 植物遺伝子組換え体作出の簡便化を目指したベクターシステムの開発2019

    • Author(s)
      福田真由, 佐藤優加, 井川智子
    • Journal Title

      月刊アグリバイオ

      Volume: 3 Pages: 40-44

    • NAID

      40022053301

    • Related Report
      2019 Research-status Report
  • [Presentation] 育種への応用を目指した、胚発生関連遺伝子による全能性コントロール2020

    • Author(s)
      佐藤優加, 井川智子
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 植物の形質転換を簡便化する遺伝子導入ベ クターの開発および再分化の評価2019

    • Author(s)
      佐藤優加, 井川智子
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 植物の形質転換の簡便化を目的とした組換えベクターの開発2017

    • Author(s)
      佐藤優加、井川智子
    • Organizer
      第81回日本植物学会大会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2022-01-27  

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