Elucidation of a sporophytic gene related to pollen killing

Research Project

Project/Area Number	17K07603
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Science in genetics and breeding
Research Institution	Kyushu University
Principal Investigator	Kubo Takahiko 九州大学, 農学研究院, 准教授 (00370148)
Project Period (FY)	2017-04-01 – 2021-03-31
Project Status	Completed (Fiscal Year 2020)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords	花粉キラー / 遺伝子 / 胞子体因子 / Oryza saitiva / indica / japonica / 植物 / 育種学
Outline of Final Research Achievements	The aim of this study is to clarify the molecular basis of pollen killer system involving a pollen killer S35 and an interacting partner gene INK found in a subspecific cross of cultivated rice (Oryza sativa ssp. japonica × indica). Positional cloning of this gene was carried out in this study. INK was found to be composed of at least two or more chromosomal subregions, and a candidate gene ORF1 was identified. In addition, investigation of the gene distribution of Ink-i in cultivars and ancestral wild species, Ink-i was shown to be originated from the ancestral wild species O. rufipogon.
Academic Significance and Societal Importance of the Research Achievements	作物の品種改良は主に、異なる品種間あるいは種・亜種間の交雑に派生する優良個体の選定、によって進められている。このような種・亜種間交雑では、花粉キラーと呼ばれる遺伝子が片親に由来する優良遺伝子の正常な伝達を阻害し、優良個体が得られにくくなることがある。本成果は、花粉キラーの分子的要因と遺伝子分布を明らかにするものであり、これによって、遺伝子伝達阻害の解消と作物品種改良の効率化につながる。