Functional analysis of lodging resistance locus prl5 in rice

• Project/Area Number: 17K07631
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Crop production science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: ISHIMARU KEN 国立研究開発法人農業・食品産業技術総合研究機構, 作物研究部門, 主席研究員 (80370641)
• Project Period (FY): 2017-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 倒伏 / イネ / 植物体支持力 / QTL / 老化 / 糖シグナル / 支持力 / NIL / 上米比率 / 半矮性 / ゲノム育種

Outline of Final Research Achievements

In this study, we elucidated the function and evaluated the valuable of prl5 enhanced lodging resistance by pushing resistance in lower parts. We found that the sugar-signaling T6P synthesis gene was the causal gene of prl5, and that differential expression is a factor determining the allele. Furthermore, it was suggested that prl5 enhances the resistance by maintaining photosynthetic ability through suppressing the expression of SnRK1, which is involved in plant senescence. The yield and taste of NIL were comparable to those of Koshihikari, suggesting its high usefulness.

Academic Significance and Societal Importance of the Research Achievements

倒伏はイネ栽培における最も重要な障害であり、コストや確実性から耐性品種の開発が最も有効な対処方法で有る。本研究において我々の研究チームは、植物体の支持力を強くして耐倒伏性を高めるprl5の原因遺伝子を特定し有用性が高いことを示した。得られた結果は、耐性系統の作出に活用できる。また、イネの植物体下部の枯れ上がりがどの様に制御されているかを分子レベルで明らかにできた。これらの結果は、老化機構の全貌解明に繋がる重要な知見である。

Research Products

• [Journal Article] How elevated CO2 affects our nutrition in rice, and how we can deal with it (2019)
  • Author(s): Ujiie Kazuhiro、Ishimaru Ken、Hirotsu Naoki、Nagasaka Seiji、Miyakoshi Yuichi、Ota Masako、Tokida Takeshi、Sakai Hidemitsu、Usui Yasuhiro、Ono Keisuke、Kobayashi Kazuhiko、Nakano Hiroshi、Yoshinaga Satoshi、Kashiwagi Takayuki、Magoshi Jun
  • Journal Title: PLOS ONE Volume: 14 Issue: 3 Pages: e0212840-e0212840
  • DOI: 10.1371/journal.pone.0212840
  • NAID: 120006871778
  • Peer Reviewed / Open Access
• [Journal Article] Partial loss-of-function of NAL1 alters canopy photosynthesis by changing the contribution of upper and lower canopy leaves in rice. (2018)
  • Author(s): Naoki Hirotsu, Kazuhiro Ujiie, Ishara Perera, Ayano Iri, Takayuki Kashiwagi & Ken Ishimaru.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 15958-15958
  • DOI: 10.1038/s41598-017-15886-5
  • Peer Reviewed / Open Access