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Development of the male sterile line of eggplant using the cytoplasm of Solanum macrocarpon

Research Project

Project/Area Number 17K07645
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticultural science
Research InstitutionSaga University

Principal Investigator

Isshiki Shiro  佐賀大学, 農学部, 教授 (40253588)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsコルヒチン核置換法 / 細胞質雄性不稔 / Solanum macrocarpon / ナス / 複二倍体 / S. macrocarpon / 雄性不稔 / 花粉稔性 / 戻し交雑 / 細胞質 / ナス属野生種
Outline of Final Research Achievements

We aimed to develop a new CMS line of eggplant using the cytoplasm of Solanum macrocarpon. Since F1 of S. macrocarpon and eggplant is sterile, in this study, F1 was treated with colchicine to produce the amphidiploid, and then the amphidiploid was self-propagated to produce self-fertilized seedlings of the amphidiploid. Using these seedlings from the amphidiploid, we aimed to develop a male sterile line with the cytoplasm of S. macrocarpon. As a result, the cytoplasmic replacement line of eggplant using the cytoplasm of S. macrocarpon has male sterility without anther dehiscence, and the development of a new male sterility line of eggplant can be expected in the future.

Academic Significance and Societal Importance of the Research Achievements

本研究成果の学術的意義や社会的意義は、ナスにおいてコルヒチン核置換法によっても雄性不稔系統の開発を目指すことができた点である。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2021 2020 2019 2018 2017

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (6 results)

  • [Journal Article] Whole Genome Sequence of Synthesized Allopolyploids in Cucumis Reveal Insights into the Genome Evolution of Allopolyploidization.2021

    • Author(s)
      Xiaqing Yu; Panqiao Wang; Ji Li; Qinzheng Zhao; Changmian Ji; Zaobing Zhu; Yufei Zhai; Xiaodong Qin; Junguo Zhou; Haiyan Yu; Xinchao Cheng; Shiro Isshiki; Molly Jahn; Jeff J Doyle; Carl-Otto Ottosen; Yulin Bai; Qinsheng Cai; Chunyan Cheng; Qunfeng Lou; Sanwen Huang; Jinfeng Chen
    • Journal Title

      Advanced Science

      Volume: 2004222 Issue: 9 Pages: 1-15

    • DOI

      10.1002/advs.202004222

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pollen fertility differences in the progenies obtained from a cross between eggplant (Solanum melongena L.) as a seed parent and eggplant cytoplasmic substitution lines as pollen parents2021

    • Author(s)
      Shiro Isshiki1, Ichiro Nakamura2, kenji Ureshino2, Md. Mizanur Rahim Khan (Corresponding author)2,*
    • Journal Title

      Australian Journal of Crop Science

      Volume: 15 Issue: 15(02):2021 Pages: 233-237

    • DOI

      10.21475/ajcs.21.15.02.p2785

    • NAID

      120007153435

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic Characterization of the Fertility Restorer (Rf) Genes and Their Linked DNA Marker of the Male Sterile Eggplant Systems Having the Cytoplasm of the Wild Species2018

    • Author(s)
      KHAN Md. Mizanur Rahim、IWAYOSHI Masaki、OGURA-TSUJITA Yuki、ISSHIKI Shiro
    • Journal Title

      Environment Control in Biology

      Volume: 56 Issue: 4 Pages: 173-176

    • DOI

      10.2525/ecb.56.173

    • NAID

      40021814109

    • ISSN
      1880-554X, 1883-0986
    • Year and Date
      2018-10-01
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Solanum virginianumを利用したナスの細胞質置換系統の後代における稔性の変化について2020

    • Author(s)
      有田隆史・岩吉真輝・辻田有紀・モハマド ミザヌル ラヒム カーン・一色司郎
    • Organizer
      園芸学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Solanum virginianumを利用したナスの細胞質置換系統の後代における稔性の変化について2020

    • Author(s)
      有田隆史・岩吉真輝・辻田有紀・モハマド ミザヌル ラヒム カーン・一色司郎
    • Organizer
      園芸学会
    • Related Report
      2019 Research-status Report
  • [Presentation] Solanum violaceum を利用した ナスの細胞質置換系統の後代における低い花粉稔性2019

    • Author(s)
      鹿田裕介・有田隆史・岩吉真輝・渡辺久修・辻田有紀・モハマド ミザヌル ラヒム カーン・一色司郎
    • Organizer
      園芸学会
    • Related Report
      2019 Research-status Report
  • [Presentation] ナス属野生種Solanum giloの細胞質をもつナスの雄性不稔系統の育成2018

    • Author(s)
      妹川知史、渡辺久修、辻田有紀、岩吉真輝、一色司郎
    • Organizer
      園芸学会
    • Related Report
      2018 Research-status Report
  • [Presentation] Solanum kurziiの細胞質を用いたナスの雄性不稔系統について2018

    • Author(s)
      鹿田裕介、渡辺久修、辻田有紀、岩吉真輝、一色司郎
    • Organizer
      園芸学会
    • Related Report
      2018 Research-status Report
  • [Presentation] ナスを種子親およびナス細胞質置換系統を花粉親として作出した後代の花粉稔性2017

    • Author(s)
      妹川知史・渡辺久修・辻田有紀・岩吉真輝・一色司郎
    • Organizer
      園芸学会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2022-01-27  

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