Growth evaluation of strigolactone-biosynthetic defective mutants in tomato
Project/Area Number |
17K07650
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Horticultural science
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Research Institution | Toyo University |
Principal Investigator |
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Project Period (FY) |
2017-04-01 – 2022-03-31
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Project Status |
Completed (Fiscal Year 2021)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | ストリゴラクトン / マイクロトム / CCD8 / MAX1 / LBO / D14 / TILLING / ゲノム編集 / D14L / CCD7 / 果実形質 / Phelipanche aegiptiaca / LC-MS/MS / 園芸学 / 植物 / 生理学 / 遺伝子 / バイオテクノロジー |
Outline of Final Research Achievements |
Shoot branching is an important trait in agriculture and horticulture because it affects the number and quality of fruits. In this study, to investigate the role of strigolactones (SLs) in fruit formation and the SL pathway in tomato, we obtained some SL-related mutants of Micro-Tom by the TILLING method or genome editing and characterized phenotypes of the mutants. Furthermore, we found that infection of the root parasitic plants was lower in the roots of SL biosynthesis mutants than in WT roots because of the low germination.
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Academic Significance and Societal Importance of the Research Achievements |
典型的SLと非典型的SLの両方を産生するトマトのSLシグナル経路を明らかにすることで、下流で複数の経路が存在する意義について検証できる。また、根寄生植物はわずかなSLに応答して発芽して宿主植物に寄生する。我々のSL生合成欠損変異体では高感度なLC-MS分析で内生SLを検出できない上、根寄生植物に根滲出液を与えても発芽がほとんど確認できなかったことから、根寄生植物抵抗性トマト品種の育種に利用できる可能性がある。
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Report
(6 results)
Research Products
(22 results)
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[Presentation] Micro-Tom CAROTENOID CLEAVAGE DIOXYGENASE 8 mutants show low infection of Phelipanche aegyptiaca2019
Author(s)
Shoko Hasegawa, Takuya Tsutsumi, Shunsuke Fukushima, Yoshihiro Okabe, Junna Saito, Mina Katayama, Masato Shindo, Yusuke Yamada, Koichiro Shimomura, Kaori Yoneyama, Kohki Akiyama, Koh Aoki, Tohru Ariizumi, Hiroshi Ezura, Shinjiro Yamaguchi, Mikihisa Umehara
Organizer
IPGSA conference 2019
Related Report
Int'l Joint Research
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