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Growth evaluation of strigolactone-biosynthetic defective mutants in tomato

Research Project

Project/Area Number 17K07650
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticultural science
Research InstitutionToyo University

Principal Investigator

Mikihisa Umehara  東洋大学, 生命科学部, 教授 (30469895)

Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsストリゴラクトン / マイクロトム / CCD8 / MAX1 / LBO / D14 / TILLING / ゲノム編集 / D14L / CCD7 / 果実形質 / Phelipanche aegiptiaca / LC-MS/MS / 園芸学 / 植物 / 生理学 / 遺伝子 / バイオテクノロジー
Outline of Final Research Achievements

Shoot branching is an important trait in agriculture and horticulture because it affects the number and quality of fruits. In this study, to investigate the role of strigolactones (SLs) in fruit formation and the SL pathway in tomato, we obtained some SL-related mutants of Micro-Tom by the TILLING method or genome editing and characterized phenotypes of the mutants. Furthermore, we found that infection of the root parasitic plants was lower in the roots of SL biosynthesis mutants than in WT roots because of the low germination.

Academic Significance and Societal Importance of the Research Achievements

典型的SLと非典型的SLの両方を産生するトマトのSLシグナル経路を明らかにすることで、下流で複数の経路が存在する意義について検証できる。また、根寄生植物はわずかなSLに応答して発芽して宿主植物に寄生する。我々のSL生合成欠損変異体では高感度なLC-MS分析で内生SLを検出できない上、根寄生植物に根滲出液を与えても発芽がほとんど確認できなかったことから、根寄生植物抵抗性トマト品種の育種に利用できる可能性がある。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (22 results)

All 2021 2020 2019 2018 2017 Other

All Journal Article (6 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (13 results) (of which Int'l Joint Research: 6 results,  Invited: 4 results) Remarks (3 results)

  • [Journal Article] ストリゴラクトン生合成遺伝子欠損トマトの利用に向けて2021

    • Author(s)
      相場北斗、長谷川翔子、梅原三貴久
    • Journal Title

      アグリバイオ

      Volume: 5 Pages: 1274-1276

    • NAID

      40022785750

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Strigolactones Decrease Leaf Angle in Response to Nutrient Deficiencies in Rice2020

    • Author(s)
      Masato Shindo, Shu Yamamoto, Koichiro Shimomura, Mikihisa Umehara
    • Journal Title

      Frontiers in Plant Science

      Volume: 11 Pages: 135-135

    • DOI

      10.3389/fpls.2020.00135

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 82018

    • Author(s)
      Hasegawa Shoko、Tsutsumi Takuya、Fukushima Shunsuke、Okabe Yoshihiro、Saito Junna、Katayama Mina、Shindo Masato、Yamada Yusuke、Shimomura Koichiro、Yoneyama Kaori、Akiyama Kohki、Aoki Koh、Ariizumi Tohru、Ezura Hiroshi、Yamaguchi Shinjiro、Umehara Mikihisa
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 19 Issue: 9 Pages: 2645-2645

    • DOI

      10.3390/ijms19092645

    • NAID

      120007133661

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 栄養欠乏に応答して増加するストリゴラクトンの役割2018

    • Author(s)
      進藤真登、梅原三貴久
    • Journal Title

      アグリバイオ

      Volume: 2 Pages: 914-916

    • NAID

      40021808178

    • Related Report
      2018 Research-status Report
  • [Journal Article] Upregulation of DWARF27 is associated with increased strigolactone levels under sulfur deficiency in rice.2018

    • Author(s)
      Masato Shindo, Koichiro Shimomura, Shinjiro Yamaguchi, Mikihisa Umehara
    • Journal Title

      Plant Direct

      Volume: 4 Issue: 4

    • DOI

      10.1002/pld3.50

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] ストリゴラクトンの生合成と生理作用2017

    • Author(s)
      梅原三貴久
    • Journal Title

      アグリバイオ

      Volume: 1 Pages: 1092-1093

    • Related Report
      2017 Research-status Report
  • [Presentation] Characterization of DWARF14 that encodes a strigolactone receptor in Micro-Tom2021

    • Author(s)
      Hokuto Aiba, Koichi Sugimoto, Ryoji Shinozaki, Hiroshi Ezura, Mikihisa Umehara
    • Organizer
      第17回日本ナス科コンソーシアム年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ゲノム編集によるトマトのストリゴラクトン受容体遺伝子DWARF14欠損変異体の作出2021

    • Author(s)
      相場北斗、杉本貢一、篠崎良仁、江面浩、梅原三貴久
    • Organizer
      植物化学調節学会第56回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Micro-Tom CAROTENOID CLEAVAGE DIOXYGENASE 8 mutants show low infection of Phelipanche aegyptiaca2019

    • Author(s)
      Shoko Hasegawa, Takuya Tsutsumi, Shunsuke Fukushima, Yoshihiro Okabe, Junna Saito, Mina Katayama, Masato Shindo, Yusuke Yamada, Koichiro Shimomura, Kaori Yoneyama, Kohki Akiyama, Koh Aoki, Tohru Ariizumi, Hiroshi Ezura, Shinjiro Yamaguchi, Mikihisa Umehara
    • Organizer
      IPGSA conference 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Phenotypic analysis of strigolactone-deficient mutants in rice and tomato2019

    • Author(s)
      Mikihisa Umehara
    • Organizer
      The 2nd International Conference on Plant and Molecular Biology
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Production of tomato resistant to infection by root parasitic plants2018

    • Author(s)
      Mikihisa Umehara
    • Organizer
      Global Summit on Plant Science & Plant Physiology (Plant Science 2018)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] D27, a strigolactone biosynthetic gene, is required for adaptation in sulfate deficient condition2017

    • Author(s)
      Masato Shindo, Koichiro Shimomura, Shinjiro Yamaguchi, Mikihisa Umehara
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Characteristics of two Slccd8 mutants in Micro-Tom2017

    • Author(s)
      Shoko Hasegawa, Nao Tsunoda, Yoshihiro Okabe, Toru Ariizumi, Hiroshi Ezura, Mikihisa Umehara
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 硫酸イオン欠乏に適応するためにD27の働きが必要である2017

    • Author(s)
      進藤真登、下村講一郎、山口信次郎、梅原三貴久
    • Organizer
      植物化学調節学会第52回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] トマトのストリゴラクトン欠損系統作出と特性評価2017

    • Author(s)
      長谷川翔子、角田奈央、岡部佳弘、有泉亨、江面浩、梅原三貴久
    • Organizer
      植物化学調節学会第52回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 硫酸イオン欠乏条件に応答して増加するストリゴラクトンの生理学的役割2017

    • Author(s)
      進藤真登、下村講一郎、山口信次郎、梅原三貴久
    • Organizer
      日本植物細胞分子生物学会第35回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] マイクロトムSlCCD8欠損系統2757の特性評価2017

    • Author(s)
      長谷川翔子、角田奈央、岡部佳弘、有泉亨、江面浩、梅原三貴久
    • Organizer
      日本植物細胞分子生物学会第35回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ストリゴラクトンの植物の成長調節作用2017

    • Author(s)
      梅原 三貴久
    • Organizer
      第6回植物二次代謝フロンティア研究会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Physiological functions of strigolactones in plant growth and development2017

    • Author(s)
      Mikihisa Umehara
    • Organizer
      The 2nd Japan-Korea-China Trilateral Joint Symposium on Plant Biotechnology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Remarks] 生命科学部 応用生物科学科 植物生長制御研究室 Umehara Lab.

    • URL

      http://www2.toyo.ac.jp/%7Eumehara/plant_biotechnology/Welcome.html

    • Related Report
      2021 Annual Research Report 2020 Research-status Report 2018 Research-status Report
  • [Remarks] 生命科学部 応用生物科学科 植物生長制御研究室 Umehara Lab.

    • URL

      http://www2.toyo.ac.jp/~umehara/plant_biotechnology/Welcome.html

    • Related Report
      2019 Research-status Report
  • [Remarks] 生命科学部 応用生物科学科 植物生長制御研究室 Umehara Lab.

    • URL

      http://www2.toyo.ac.jp/~umehara/plant_biotechnology/Welcome.html

    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2023-01-30  

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