Genes and their transcriptional regulation in salicylic acid biosynthesis mediated by peroxisomal beta-oxidation

• Project/Area Number: 17K07665
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Shinshu University
• Principal Investigator: Katou Shinpei 信州大学, 学術研究院農学系, 准教授 (10533614)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 病害抵抗性 / サリチル酸 / 生合成 / ペルオキシソーム / β酸化 / 生合成経路

Outline of Final Research Achievements

Salicylic acid (SA) plays an important role in plant resistance to pathogens. In this study, using tobacco and Nicotiana benthamiana as experimental materials, we had two main findings described below. (1) Two enzyme genes induced during hypersensitive responses and genes encoding peroxisomal β-oxidation enzymes are required for SA production induced by a pathogen-derived signal. (2) Two CBP60-type transcription factors are required for expression of genes described in (1) induced by a pathogen-derived signal.

Academic Significance and Societal Importance of the Research Achievements

近年、安全で耐性菌の出現しにくい次世代型の農薬として、植物の病害抵抗反応を誘導することにより病害を防除する“プラントアクティベーター農薬”が注目されている。しかしながら、プラントアクティベーター農薬の開発は難易度が高く、実際に登録されている化合物は数種類に過ぎない。本研究で同定されたSA合成に必要な酵素の基質（SAの前駆化合物）等が明らかになれば、プラントアクティベーター農薬の開発に利用可能であると期待される。

Research Products

• [Journal Article] Transcriptional induction of capsidiol synthesis genes by wounding can promote pathogen signal-induced capsidiol synthesis (2019)
  • Author(s): Kojima Tomoya、Asakura Nobuhide、Hasegawa Shiori、Hirasawa Taishi、Mizuno Yuri、Takemoto Daigo、Katou Shinpei
  • Journal Title: BMC Plant Biology Volume: 19 Issue: 1 Pages: 576-576
  • DOI: 10.1186/s12870-019-2204-1
  • Peer Reviewed / Open Access
• [Journal Article] Isolation of Natural Fungal Pathogens from Marchantia polymorpha Reveals Antagonism between Salicylic Acid and Jasmonate during Liverwort?Fungus Interactions (2019)
  • Author(s): Matsui Hidenori、Iwakawa Hidekazu、Hyon Gang-Su、Yotsui Izumi、Katou Shinpei、Monte Isabel、Nishihama Ryuichi、Franzen Rainer、Solano Roberto、Nakagami Hirofumi
  • Journal Title: Plant and Cell Physiology Volume: 61 Issue: 2 Pages: 265-275
  • DOI: 10.1093/pcp/pcz187
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Comparative analysis of plant isochorismate synthases reveals structural mechanisms underlying their distinct biochemical properties (2018)
  • Author(s): Yokoo Shohei、Inoue Seiya、Suzuki Nana、Amakawa Naho、Matsui Hidenori、Nakagami Hirofumi、Takahashi Akira、Arai Ryoichi、Katou Shinpei
  • Journal Title: Bioscience Reports Volume: 38 Issue: 2
  • DOI: 10.1042/bsr20171457
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Salicylic Acid and Jasmonic Acid Pathways are Activated in Spatially Different Domains Around the Infection Site During Effector-Triggered Immunity in Arabidopsis thaliana (2017)
  • Author(s): Betsuyaku Shigeyuki、Katou Shinpei、Takebayashi Yumiko、Sakakibara Hitoshi、Nomura Nobuhiko、Fukuda Hiroo
  • Journal Title: Plant and Cell Physiology Volume: 59 Issue: 1 Pages: 8-16
  • DOI: 10.1093/pcp/pcx181
  • NAID: 120007134518
  • Peer Reviewed / Open Access
• [Journal Article] EIN2-mediated signaling is involved in pre-invasion defense in Nicotiana benthamiana against potato late blight pathogen, Phytophthora infestans (2017)
  • Author(s): Rin Soriya、Mizuno Yuri、Shibata Yusuke、Fushimi Mayuka、Katou Shinpei、Sato Ikuo、Chiba Sotaro、Kawakita Kazuhito、Takemoto Daigo
  • Journal Title: Plant Signaling & Behavior Volume: 12 Issue: 4 Pages: e1300733-e1300733
  • DOI: 10.1080/15592324.2017.1300733
  • Peer Reviewed
• [Presentation] サリチル酸合成関連遺伝子の細胞内局在解析および機能解析 (2020)
  • Author(s): 田崎光佑、小守啓友、今野沙弥香、加藤新平
  • Organizer: 令和2年度日本植物病理学会大会
• [Presentation] タバコのCBP60型転写因子により制御される遺伝子の同定 (2020)
  • Author(s): Nguyen Thi Tuyet Nhung、髙木公美子、加藤新平
  • Organizer: 令和2年度日本植物病理学会大会
• [Presentation] 傷害によるカプシジオール合成遺伝子群の転写誘導は病原体シグナルによるカプシジオール合成を増強する (2020)
  • Author(s): 長谷川詩織、平沢大志、小島知弥、朝倉信英、水野邑里、竹本大吾、加藤新平
  • Organizer: 令和2年度日本植物病理学会大会
• [Presentation] サリチル酸の合成経路の候補遺伝子の過剰発現および細胞内局在解析 (2019)
  • Author(s): 小守啓友、今野沙弥香、加藤新平
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] タバコのCBP60 型転写因子であるNtCBP60gとNtSARD1 により制御される遺伝子の同定 (2019)
  • Author(s): Tuyet Nhung Nguyen Thi、髙木公美子、加藤新平
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] タバコのサリチル酸合成に相関して発現する遺伝子の機能解析 (2018)
  • Author(s): 小守啓友、今野沙弥香、加藤新平
  • Organizer: 平成30年度日本植物病理学会大会
• [Presentation] Establishment of the plant-microbe interaction research in Marchantia polymorpha (2018)
  • Author(s): Hidekazu Iwakawa, Izumi Yotsui, Hidenori Matsui, Yuko Nomura, Katharina Kramer, Anne Harzen, Takehiko Kanazawa, Ryuichi Nishihama, Shinpei Katou, Takashi Ueda, Takayuki Kohchi, Hirofumi Nakagami
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] Establishment of the plant-microbe interaction research in Marchantia polymorpha (2017)
  • Author(s): Hidekazu Iwakawa, Izumi Yotsui, Hidenori Matsui, Yuko Nomura, Katharina Kramer, Anne Harzen, Takehiko Kanazawa, Ryuichi Nishihama, Shinpei Katou, Takashi Ueda, Takayuki Kohchi, Hirofumi Nakagami
  • Organizer: The 65th NIBB Conference Renaissance of Marchantia polymorpha ‐the genome and beyond‐
  • Int'l Joint Research
• [Remarks] 信州大学学術情報オンラインシステムSOAR
  • URL: http://soar-rd.shinshu-u.ac.jp/profile/ja.HmAejFkV.html
• [Remarks] 信州大学学術情報オンラインシステムSOAR 研究者総覧
  • URL: http://soar-rd.shinshu-u.ac.jp/profile/ja.HmAejFkV.html