Role and evolution of homeobox transcription factor in pathogenicity of plant pathogenic fungi

• Project/Area Number: 17K07669
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: The University of Shiga Prefecture
• Principal Investigator: Suzuki Kazumi 滋賀県立大学, 環境科学部, 教授 (90390880)
• Co-Investigator(Kenkyū-buntansha): 泉津 弘佑 滋賀県立大学, 環境科学部, 講師 (20579263)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 植物病原菌 / ホメオボックス遺伝子 / 病原性発現 / 付着器形成 / 付着器侵入 / 遺伝子破壊 / バイオトロフィー / ネクロトロフィー / 病原性発言 / 付着器の形態分化 / 侵入菌糸の形態分化 / ホメオボックス転写因子 / 胞子形成 / 植物病原菌類 / 病原性関連遺伝子 / 付着器

Outline of Final Research Achievements

Genome analysis revealed that the plant pathogenic fungus, Colletotrichum orbiculare, causal agent of cucumber anthracnose, has 10 homeobox genes. All of ten genes were disrupted using homologous recombination and the function of each homeobox gene was estimated through phenotypic analysis. Results show that 5 homeobox genes are involved in diferrent morphogenetic processes during plat infection and that these genes play important roles in pathogenicity of this fungus as pathogenicity related genes. Furthermore,these genes are evolutionally conserved since similar functuion was observed in homologues of other plant pathogenic fungi.

Academic Significance and Societal Importance of the Research Achievements

学術的意義としては，これまで十分に明らかにされていなかった，植物病原菌の病原性発現におけるホメオボックス転写因子の機能や役割および進化が明らかとなった。今後の研究の進展により，ホメオボックス転写因子を含む細胞内シグナル伝達機構の解明が期待される。また，社会的意義としては，これらの成果は農業上重要な植物病害における新しい防除方法や新しい殺菌剤の開発につながる有益な知見を提供するものと考えられる。

Research Products

• [Journal Article] Homeobox transcriptional factor CoHox4 regulates hyphal growth, conidial morphology and appressorial formation in <i>Colletotrichum orbiculare</i> (2020)
  • Author(s): 小幡善也，横山 綾，泉津弘佑，入江俊一，鈴木一実
  • Journal Title: Japanese Journal of Phytopathology Volume: 86 Issue: 2 Pages: 85-96
  • DOI: 10.3186/jjphytopath.86.85
  • NAID: 130007884441
  • ISSN: 0031-9473, 1882-0484
  • Year and Date: 2020-05-25
  • Peer Reviewed
• [Journal Article] Homeobox transcription factor CoHox2 regulates conidiation and seta formation in <i>Colletotrichum orbiculare</i> (2019)
  • Author(s): 小幡善也，横山 綾，泉津弘佑，入江俊一，鈴木一実
  • Journal Title: Japanese Journal of Phytopathology Volume: 85 Issue: 4 Pages: 334-344
  • DOI: 10.3186/jjphytopath.85.334
  • NAID: 130007771908
  • ISSN: 0031-9473, 1882-0484
  • Year and Date: 2019-11-25
  • Peer Reviewed
• [Journal Article] Homeobox transcription factor CoHox1 is required for the morphigenesis of infection hyphae in host plants and pathogenicity in Colletotrichum orbiculare (2019)
  • Author(s): Yokoyama A, Izumitsu K, Irie T, Suzuki K
  • Journal Title: Mycoscience Volume: 90 Issue: 2 Pages: 110-115
  • DOI: 10.1016/j.myc.2018.11.001
  • Peer Reviewed
• [Journal Article] Homeobox transcription factor CoHox3 is essential for appressorium formation in the plant pathogenic fungus Colletotrichum orbiculare (2018)
  • Author(s): Yokoyama A, Izumitsu K, Sumita T, Tanaka C, Irie T, Suzuki K
  • Journal Title: Mycoscience Volume: 印刷中 Issue: 5 Pages: 353-362
  • DOI: 10.1016/j.myc.2018.02.001
  • NAID: 40021666044
  • Peer Reviewed
• [Presentation] ウリ類炭疽病菌のホメオボックス転写因子CoHox4は栄養菌糸生育，分生胞子の形態，付着器形成および病原性に関与する (2017)
  • Author(s): 小幡善也，横山 綾，泉津弘佑，入江俊一，鈴木一実
  • Organizer: 平成29年度日本植物病理学会大会
• [Presentation] ウリ類炭疽病菌のホメオボックス転写因子CoHox4は多様な形態分化および病原性に関与する (2017)
  • Author(s): 小幡善也，横山 綾，泉津弘佑，入江俊一，鈴木一実
  • Organizer: 第17回糸状菌分子生物学コンファレンス