Development of a method to reduce deoxynivalenol in post-harvest forage corn by mycotoxin-degrading bacteria.

• Project/Area Number: 17K07674
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Koitabashi Motoo 国立研究開発法人農業・食品産業技術総合研究機構, 中央農業研究センター, スマート農業コーディネーター (10355662)
• Co-Investigator(Kenkyū-buntansha): 黄川田 智洋 国立研究開発法人農業・食品産業技術総合研究機構, 北海道農業研究センター, 主任研究員 (90355131)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: かび毒分解菌 / デオキシニバレノール / 飼料用トウモロコシ / トウモロコシ / サイレージ / かび毒 / 分解細菌

Outline of Final Research Achievements

The reduction effect of a bacterial strain that degrades deoxynivalenol produced by Fusarium head blight in a medium was verified in a corn field for feed. After 10 days of silking stage, conidial suspensions of the pathogens were inoculated, and after 10 days, 10 degradative bacteria were inoculated into the bracts 4 times every 10 days (2017, Sapporo). The grains were dried and ground to measure DON concentration.
The concentration of DON was 11.67 ppm in the untreated group, but was reduced to 2.53 ppm in the inoculated group with Nocardioides sp. In addition, significant spraying effect was observed in 4 strains. When these 5 strains were repeated in 2018, 3 strains showed a significant reduction effect, and these strains also showed a significant reduction effect in the 2019.

Academic Significance and Societal Importance of the Research Achievements

DON分解菌の圃場での効果を３年間にわたって検証し、３菌株に低減効果があることを確認した。これらの成果を基に、化学的防除手段のない飼料用トウモロコシにおける赤かび病のによるかび毒低減に関する生物的防除技術開発の可能性が示唆された。

Research Products

• [Journal Article] Draft Genome Sequence of Deoxynivalenol-Degrading Actinomycete Nocardioides sp. Strain LS1, Isolated from Wheat Leaves in Japan (2019)
  • Author(s): Morimura Hiroyuki、Uesaka Kazuma、Ito Michihiro、Yoshida Shigenobu、Koitabashi Motoo、Tsushima Seiya、Sato Ikuo
  • Journal Title: Microbiology Resource Announcements Volume: 8 Issue: 10 Pages: 10-10
  • DOI: 10.1128/mra.01650-18
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] トウモロコシ赤かび病に罹病した飼料用トウモロコシへのデオキシニバレノール分解細菌の散布効果 (2019)
  • Author(s): 小板橋 基夫、黄川田 智洋、菅原 幸哉、吉田 重信、伊藤通浩、佐藤育男、對馬誠也
  • Organizer: 日本植物病理学会 85(3),263
• [Presentation] トウモロコシ赤かび病に罹病した飼料用 トウモロコシへのデオキシニバレノール 分解細菌の散布効果 (2019)
  • Author(s): 小板橋基夫・黄川田智洋・菅原幸哉・吉田重信・ 伊藤通浩・佐藤育男・對馬誠也
  • Organizer: 日本植物病理学会
• [Presentation] コムギ赤かび病で有効性が確認されたデオキシニバレノール分解細菌の飼料用トウモロコシへの応用 (2017)
  • Author(s): 小板橋 基夫、黄川田 智洋、菅原 幸哉、吉田 重信、伊藤通浩、佐藤育男、對馬誠也
  • Organizer: 日本植物病理学会報 83(3),202-202