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Identification of genes for disease protection by analyzing plant-pathogen interactions at a cellular level

Research Project

Project/Area Number 17K07679
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant protection science
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

ASAI SHUTA  国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (30723580)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords罹病性遺伝子 / 病害防除 / トランスクリプトーム / べと病菌 / シロイヌナズナ
Outline of Final Research Achievements

In the relationship between host plants and pathogens, there are genes on the host side, so-called susceptibility genes, that are required for infection by pathogens. Mutation or loss of a susceptibility gene can limit the ability of the pathogen to cause disease. I am therefore conducting research on susceptibility genes as useful genes that can contribute to disease control. In this study, I obtained susceptibility gene candidates to downy mildew through forward genetic approach and cell type-specific transcriptome approach using Arabidopsis thaliana, a model plant. I am proceeding with their functional analysis and aiming to apply them to crops.

Academic Significance and Societal Importance of the Research Achievements

べと病は、キャベツなどのアブラナ科作物や、レタス、ホウレンソウ、更にはヒマワリなどでの重要病害として広く知られており、古くから抵抗性品種が利用されてきた。しかし、これら抵抗性品種の多くは、gene-for-geneで制御されるような抵抗性遺伝子による抵抗性であり、新レースの出現と抵抗性打破のリスクを常に招いている。罹病性遺伝子は、病原菌にとって宿主侵入前後における必須因子であることが予想されるため、持続的な病害抵抗性に貢献しうる遺伝資源と期待される。また、罹病性遺伝子は劣性遺伝子であるため、有用作物において遺伝子組換え以外の手法で遺伝子破壊をすることで、耐病性を付与することが可能である。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (28 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (7 results) Journal Article (6 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 5 results,  Acknowledgement Compliant: 2 results) Presentation (14 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (1 results)

  • [Int'l Joint Research] マックスプランク研究所(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] センズベリー研究所(英国)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] カリフォルニア大学バークレー校(米国)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] ベルリン自由大学(ドイツ)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] センズベリー研究所(英国)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] マックスプランク研究所(ドイツ)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] ミネソタ大学(米国)

    • Related Report
      2017 Research-status Report
  • [Journal Article] High-Quality Draft Genome Sequence of Fusarium oxysporum f. sp. cubense Strain 160527, a Causal Agent of Panama Disease2019

    • Author(s)
      Asai Shuta、Ayukawa Yu、Gan Pamela、Masuda Sachiko、Komatsu Ken、Shirasu Ken、Arie Tsutomu
    • Journal Title

      Microbiology Resource Announcements

      Volume: 8 Issue: 29

    • DOI

      10.1128/mra.00654-19

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A downy mildew effector evades recognition by polymorphism of expression and subcellular localization2018

    • Author(s)
      1)Asai S., Furzer O.J., Cevik V., Kim D.S., Ishaque N., Goritschnig S., Staskawicz B.J., Shirasu K. and Jones J.D.G.
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 5192-5192

    • DOI

      10.1038/s41467-018-07469-3

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Arabidopsis downy mildew effector HaRxL106 suppresses plant immunity by binding to RADICAL-INDUCED CELL DEATH12018

    • Author(s)
      2)Wirthmueller L., Asai S., Rallapalli G., Sklenar J., Fabro G., Kim D.S., Lintermann R., Jaspers P., Wrzaczek M., Kangasjärvi J., MacLean D., Menke F.L.H., Banfield M.J. and Jones J.D.G.
    • Journal Title

      New Phytologist

      Volume: 220 Issue: 1 Pages: 232-248

    • DOI

      10.1111/nph.15277

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 圃場病原ゲノミクス -土壌診断法の開発にむけて-2018

    • Author(s)
      浅井秀太
    • Journal Title

      植物細菌病談話会論文集(第28号)ISSN 1346-5767

      Volume: 28 Pages: 34-39

    • Related Report
      2018 Research-status Report
  • [Journal Article] The highly buffered Arabidopsis immune signaling network conceals the functions of its components2017

    • Author(s)
      Hillmer R.A., Tsuda K., Rallapalli G., Asai S., Truman W., Papke M.D., Sakakibara H., Jones J.D.G., Myers C.L. and Katagiri F.
    • Journal Title

      PLoS Genetics

      Volume: 13(5) Issue: 5 Pages: e1006639-e1006639

    • DOI

      10.1371/journal.pgen.1006639

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Albugo-imposed changes to tryptophanderived antimicrobial metabolite biosynthesis may contribute to suppression of non-host resistance to Phytophthora infestans in Arabidopsis thaliana2017

    • Author(s)
      Prince D.C., Rallapalli G., Xu D., Schoonbeek H.-J., Cevik V., Asai S., Kemen E., Cruz-Mireles N., Kemen A., Belhaj K., Schornack S., Kamoun S., Holub E.B., Halkier B.A. and Jones J.D.G.
    • Journal Title

      BMC Biology

      Volume: 15:20 Issue: 1 Pages: 1-22

    • DOI

      10.1186/s12915-017-0360-z

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 比較ゲノム解析を通したフザリウム分化型解析マーカーの構築と応用2020

    • Author(s)
      浅井秀太
    • Organizer
      令和2年度植物病理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 比較ゲノム解析を通したフザリウム分化型解析マーカーの構築2019

    • Author(s)
      浅井秀太
    • Organizer
      令和元年度植物病理学会関東部会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 圃場病原ゲノミクス -土壌診断法の開発にむけて-2019

    • Author(s)
      浅井秀太
    • Organizer
      第12回フザリウム研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Identification and characterization of revertants of the dde2/ein2/pad4/sid2-quadruple mutant, which exhibit disease resistance2019

    • Author(s)
      浅井秀太
    • Organizer
      XVIII International Congress on Molecular Plant-Microbe Interactions
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] dde2/ein2/pad4/sid2復帰突然変異体の同定および表現型解析2019

    • Author(s)
      浅井秀太
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Identification and characterization of revertants of the dde2/ein2/pad4/sid2-quadruple mutant, which exhibit disease resistance2019

    • Author(s)
      浅井秀太
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 植物病原菌の巧妙な認識回避の“術”2018

    • Author(s)
      浅井秀太
    • Organizer
      第4回農学中手の会
    • Related Report
      2018 Research-status Report
  • [Presentation] フザリウム分化型解析マーカー構築に向けた比較ゲノム解析2018

    • Author(s)
      浅井秀太
    • Organizer
      平成30年度植物病理学会関東部会
    • Related Report
      2018 Research-status Report
  • [Presentation] 圃場病原ゲノミクス -土壌診断法の開発にむけて-2018

    • Author(s)
      浅井秀太
    • Organizer
      平成30年度(第28回)植物細菌病談話会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 細胞レベルでの宿主病原菌相互作用機構解析による病害防除に向けた有用遺伝子の探索2018

    • Author(s)
      浅井秀太
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 細胞レベルでの宿主病原菌相互作用機構の解析2017

    • Author(s)
      浅井秀太
    • Organizer
      第3回農学中手の会
    • Related Report
      2017 Research-status Report
  • [Presentation] 罹病性遺伝子の同定に向けたdde2/ein2/pad4/sid2の復帰突然変異体のスクリーニング2017

    • Author(s)
      浅井秀太
    • Organizer
      平成29年度日本植物病理学会関東部会
    • Related Report
      2017 Research-status Report
  • [Presentation] 病原ゲノミクスによる土壌診断法の開発2017

    • Author(s)
      浅井秀太
    • Organizer
      平成29年度植物感染生理談話会
    • Related Report
      2017 Research-status Report
  • [Presentation] Subtilisin-like proteaseは植物病原細菌に対する抵抗性を正に制御する2017

    • Author(s)
      浅井秀太
    • Organizer
      平成29年度日本植物病理学会大会
    • Related Report
      2017 Research-status Report
  • [Remarks] 理化学研究所植物免疫研究グループ

    • URL

      http://plantimmunity.riken.jp/index_ja.html

    • Related Report
      2019 Annual Research Report 2018 Research-status Report 2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2022-02-22  

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