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Physiological functions of ALMT-family malate transporters specifically expressed in organs of tomato

Research Project

Project/Area Number 17K07697
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionOkayama University

Principal Investigator

Sasaki Takayuki  岡山大学, 資源植物科学研究所, 准教授 (60362985)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsリンゴ酸輸送体 / トマト / ALMTファミリー / アルミニウム / 電気生理学 / 果実 / 孔辺細胞 / ALMT / アニオン輸送
Outline of Final Research Achievements

In this study, two tomato ALMT-family malate transporter genes expressed in fruit and leaf were analyzed. The SlALMT gene in fruit was found to be expressed in the vascular bundles during the early stage of maturation. The SlALMT transporter exhibited the efflux of malate as well as dicarboxylic acids, and the influx of nitrate and chloride. The transgenic suppresser were constructed and examined several metabolites. Malate content in mature green fruit was not different between wild type and the suppressor lines. The SlALMT gene in leaf was found to be expressed in guard cells. The SlALMT transporter exhibited the malate-efflux activated by extracellular malate concentration.

Academic Significance and Societal Importance of the Research Achievements

ALMTファミリー輸送体は植物特異的なアニオン輸送体として多くの研究が進められているが,その性質の多くは未解明である.申請者は,アルミニウム耐性遺伝子のTaALMT1,気孔閉口に関わるAtALMT12など,世界に先駆けて研究を進めてきた.本研究課題では,モデル作物のトマトにおいて,果実を含めた器官・組織・細胞で発現するALMTファミリーの輸送機能と,関与する生理機能との関係を明らかにした.この成果は,作物がもつ様々なタイプのALMT輸送体の性質を理解し,活用することで,環境ストレス耐性の付与,農作物の成長制御と品質向上への応用を可能にする.

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (19 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (13 results) (of which Int'l Joint Research: 2 results) Remarks (2 results)

  • [Int'l Joint Research] ネブラスカ大学リンカーン校(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ネブラスカ大学リンカーン校(米国)

    • Related Report
      2018 Research-status Report
  • [Journal Article] AtALMT3 is Involved in Malate Efflux Induced by Phosphorus Deficiency in Arabidopsis thaliana Root Hairs2018

    • Author(s)
      Maruyama Hayato、Sasaki Takayuki、Yamamoto Yoko、Wasaki Jun
    • Journal Title

      Plant and Cell Physiology

      Volume: 60 Issue: 1 Pages: 107-115

    • DOI

      10.1093/pcp/pcy190

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Aluminium-induced cell death requires upregulation of NtVPE1 gene coding vacuolar processing enzyme in tobacco ( Nicotiana tabacum L.)2018

    • Author(s)
      Kariya Koki、Tsuchiya Yoshiyuki、Sasaki Takayuki、Yamamoto Yoko
    • Journal Title

      Journal of Inorganic Biochemistry

      Volume: 181 Pages: 152-161

    • DOI

      10.1016/j.jinorgbio.2017.09.008

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Functional analyses of ALMT malate transporters expressed in guard cells2020

    • Author(s)
      Takayuki Sasaki, Izumi C. Mori, Michiyo Ariyoshi, Yoko Yamamoto
    • Organizer
      日本植物生理学会(大阪)
    • Related Report
      2019 Annual Research Report
  • [Presentation] トマトの気孔で発現するALMT 輸送体の機能解析2019

    • Author(s)
      佐々木孝行,有吉美智代,山本洋子,森泉
    • Organizer
      日本土壌肥料学会(静岡)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物ALMTファミリーの輸送機能の特性2019

    • Author(s)
      佐々木孝行,森泉,有吉美智代,山本洋子
    • Organizer
      日本植物学会(仙台)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物のALMT輸送体研究から解ってきたこと2019

    • Author(s)
      佐々木孝行
    • Organizer
      植物の栄養研究会 第5回研究交流会(広島)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Functional analyses of ALMT-family malate transporters in tomato2019

    • Author(s)
      Takayuki Sasaki, Michiyo Ariyoshi, Toshihiro Obata, Izumi C. Mori, Yoko Yamamoto
    • Organizer
      日本植物生理学会
    • Related Report
      2018 Research-status Report
  • [Presentation] トマトにおけるリンゴ酸輸送体ALMTファミリーの多様性2018

    • Author(s)
      佐々木孝行, 有吉美智代, 森泉, 山本洋子
    • Organizer
      日本土壌肥料学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 植物のALMTリンゴ酸輸送体の多様性2018

    • Author(s)
      佐々木孝行, 有吉美智代, 森泉, 山本洋子
    • Organizer
      日本植物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] シロバナルーピンのクラスター根で発現するMATEファミリートランスポーターの解析2018

    • Author(s)
      古谷浩章, 花城清俊, 藤井友美, 西田翔, 佐々木孝行, 和崎淳
    • Organizer
      第4回植物の栄養研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] Characterization of LaMATE-PI1, a candidate of citrate transporter isolated from cluster roots of white lupin.2018

    • Author(s)
      Hiroaki Furutani, Kiyotoshi Hanashiro, Yumi Fujii, Hayato Maruyama, Takayuki Sasaki, Jun Wasaki
    • Organizer
      Symposium on Phosphorus in Soils and Plants
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] アルミニウムの毒性ならびに耐性機構-植物培養細胞を用いたアプローチ-2017

    • Author(s)
      山本洋子、苅谷耕輝、土屋善幸、佐々木孝行
    • Organizer
      第28回日本微量元素学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] アルミニウムによるタバコの根伸長阻害におけるスクロース輸送体遺伝子の関わり2017

    • Author(s)
      苅谷耕輝、土屋善幸、佐々木孝行、山本洋子
    • Organizer
      日本土壌肥料学会
    • Related Report
      2017 Research-status Report
  • [Presentation] タバコ培養細胞におけるアルミニウム耐性とERF(エチレン応答因子)ファミリー転写調節因子との関係2017

    • Author(s)
      土屋善幸、苅谷耕輝、佐々木孝行、山本洋子
    • Organizer
      日本土壌肥料学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Organic-Acid Transporters in Cluster Roots of White Lupin Induced under Phosphorus Deficiency2017

    • Author(s)
      Hiroaki Furutani, Kiyotoshi Hanashiro, Yumi Fujii, Hayato Maruyama, Takayuki Sasaki, Jun Wasaki
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Remarks] 植物分子生理学グループ

    • URL

      http://www.rib.okayama-u.ac.jp/Plant_Mol_Physiol/index-j.html

    • Related Report
      2019 Annual Research Report
  • [Remarks]

    • URL

      http://www.rib.okayama-u.ac.jp/Plant_Mol_Physiol/index-j.html

    • Related Report
      2018 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

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