Physiological functions of ALMT-family malate transporters specifically expressed in organs of tomato
| Project/Area Number |
17K07697
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Okayama University |
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Principal Investigator |
Sasaki Takayuki 岡山大学, 資源植物科学研究所, 准教授 (60362985)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | リンゴ酸輸送体 / トマト / ALMTファミリー / アルミニウム / 電気生理学 / 果実 / 孔辺細胞 / ALMT / アニオン輸送 |
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| Outline of Final Research Achievements |
In this study, two tomato ALMT-family malate transporter genes expressed in fruit and leaf were analyzed. The SlALMT gene in fruit was found to be expressed in the vascular bundles during the early stage of maturation. The SlALMT transporter exhibited the efflux of malate as well as dicarboxylic acids, and the influx of nitrate and chloride. The transgenic suppresser were constructed and examined several metabolites. Malate content in mature green fruit was not different between wild type and the suppressor lines. The SlALMT gene in leaf was found to be expressed in guard cells. The SlALMT transporter exhibited the malate-efflux activated by extracellular malate concentration.
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| Academic Significance and Societal Importance of the Research Achievements |
ALMTファミリー輸送体は植物特異的なアニオン輸送体として多くの研究が進められているが，その性質の多くは未解明である．申請者は，アルミニウム耐性遺伝子のTaALMT1，気孔閉口に関わるAtALMT12など，世界に先駆けて研究を進めてきた．本研究課題では，モデル作物のトマトにおいて，果実を含めた器官・組織・細胞で発現するALMTファミリーの輸送機能と，関与する生理機能との関係を明らかにした．この成果は，作物がもつ様々なタイプのALMT輸送体の性質を理解し，活用することで，環境ストレス耐性の付与，農作物の成長制御と品質向上への応用を可能にする．
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Report
(4 results)
Research Products
(19 results)
