Molecular mechanism of prospore membrane extension in budding yeast: protein dephosphorylation and regulation of phospholipid metabolism
| Project/Area Number |
17K07712
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Tachikawa Hiroyuki 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (60251576)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 生体膜 / リン脂質 / 出芽酵母 / 胞子形成 / 細胞分化 / 脱リン酸化酵素 / 脂質輸送 / 環境応答 / 微生物 |
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| Outline of Final Research Achievements |
Sporulation of budding yeast is a dynamic process of intracellular reorganization. In this study, to know the mechanism of de novo membrane formation in the cell, we analyzed the formation of the prospore membrane which appears during spore formation. As to the protein phosphatase type I complex which functions in this process, we tried to identify target protein and obtained some candidates. As to Vps13 containing complex, we showed that it forms the ER-prospore membrane contact sites with other contact site proteins, and functions in regulation of the levels of phospholipids and prospore membrane extension. This study contributed to our understanding of function of Vps13 in membrane formation.
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| Academic Significance and Societal Importance of the Research Achievements |
細胞内新規膜形成は、細胞内で普遍的に見られる現象で、オートファジー、繊毛形成、植物の細胞板形成などがあげられる。出芽酵母の前胞子膜形成は、オートファジーとは全く異なるセットのタンパク質によって起きる細胞内新規膜形成の過程であり、１つのモデルとなると考えらえる。本研究は、真核生物に広く保存された１型脱リン酸化酵素およびVps13、そしてPI4Pの前胞子膜形成における働きの一端を明らかにするものであり、普遍的な膜形成機構の理解に繋がる。また、ヒトVps13ホモログは神経変性疾患の原因遺伝子であり、疾患の分子基盤の理解に繋がる可能性がある。
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Dynamic localization of a yeast development-specific PP1 complex during prospore membrane formation is dependent on multiple localization signals and complex formation.2017
Author(s)
Tsuyoshi S Nakamura, Yumi Numajiri, Yuuya Okumura, Junji Hidaka, Takayuki Tanaka, Ichiro Inoue, Yasuyuki Suda, Tetsuo Takahashi, Hideki Nakanishi, Xiao-Dong Gao, Aaron M Neiman, and Hiroyuki Tachikawa
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Journal Title
Mol. Biol. Cell
Volume: 28
Issue: 26
Pages: 3881-3895
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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