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Molecular mechanism of prospore membrane extension in budding yeast: protein dephosphorylation and regulation of phospholipid metabolism

Research Project

Project/Area Number 17K07712
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionThe University of Tokyo

Principal Investigator

Tachikawa Hiroyuki  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (60251576)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords生体膜 / リン脂質 / 出芽酵母 / 胞子形成 / 細胞分化 / 脱リン酸化酵素 / 脂質輸送 / 環境応答 / 微生物
Outline of Final Research Achievements

Sporulation of budding yeast is a dynamic process of intracellular reorganization. In this study, to know the mechanism of de novo membrane formation in the cell, we analyzed the formation of the prospore membrane which appears during spore formation. As to the protein phosphatase type I complex which functions in this process, we tried to identify target protein and obtained some candidates. As to Vps13 containing complex, we showed that it forms the ER-prospore membrane contact sites with other contact site proteins, and functions in regulation of the levels of phospholipids and prospore membrane extension. This study contributed to our understanding of function of Vps13 in membrane formation.

Academic Significance and Societal Importance of the Research Achievements

細胞内新規膜形成は、細胞内で普遍的に見られる現象で、オートファジー、繊毛形成、植物の細胞板形成などがあげられる。出芽酵母の前胞子膜形成は、オートファジーとは全く異なるセットのタンパク質によって起きる細胞内新規膜形成の過程であり、1つのモデルとなると考えらえる。本研究は、真核生物に広く保存された1型脱リン酸化酵素およびVps13、そしてPI4Pの前胞子膜形成における働きの一端を明らかにするものであり、普遍的な膜形成機構の理解に繋がる。また、ヒトVps13ホモログは神経変性疾患の原因遺伝子であり、疾患の分子基盤の理解に繋がる可能性がある。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (26 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (6 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (18 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] Stony Brook University(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] 江南大学(中国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] 江南大学(中国)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] Stony Brook University(米国)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] Jiangnan University(China)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] Stony Brook University(米国)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Dynamic localization of a yeast development-specific PP1 complex during prospore membrane formation is dependent on multiple localization signals and complex formation.2017

    • Author(s)
      Tsuyoshi S Nakamura, Yumi Numajiri, Yuuya Okumura, Junji Hidaka, Takayuki Tanaka, Ichiro Inoue, Yasuyuki Suda, Tetsuo Takahashi, Hideki Nakanishi, Xiao-Dong Gao, Aaron M Neiman, and Hiroyuki Tachikawa
    • Journal Title

      Mol. Biol. Cell

      Volume: 28 Issue: 26 Pages: 3881-3895

    • DOI

      10.1091/mbc.e17-08-0521

    • NAID

      120007134596

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Activation of Rab GTPase Sec4 by its GEF Sec2 is required for prospore membrane formation during sporulation in yeast Saccharomyces cerevisiae2017

    • Author(s)
      Suda Yasuyuki、Tachikawa Hiroyuki、Inoue Ichiro、Kurita Tomokazu、Saito Chieko、Kurokawa Kazuo、Nakano Akihiko、Irie Kenji
    • Journal Title

      FEMS Yeast Res.

      Volume: 18 Issue: 1 Pages: 1-1

    • DOI

      10.1093/femsyr/fox095

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] 出芽酵母の胞子形成における Vps13 の解析2020

    • Author(s)
      藤枝祐二 棟重賢治 中村 毅 須田恭之 舘川宏之
    • Organizer
      日本農芸化学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] PP1結合タンパク質Ysw1の出芽酵母前胞子膜形成における役割2019

    • Author(s)
      棟重賢治 中村毅 藤枝祐二 須田恭之 舘川宏之
    • Organizer
      日本細胞生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 須田恭之 舘川宏之 黒川量雄 中野明彦 入江賢児2019

    • Author(s)
      出芽酵母減数分裂における膜交通関連オルガネラの再構成
    • Organizer
      日本細胞生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 出芽酵母の胞子が非貪食細胞において取り込まれる機構の解析2019

    • Author(s)
      Yang Yan Wang Qin Liu Guoyu 舘川宏之 高暁冬 中西秀樹
    • Organizer
      日本細胞生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] PP1結合タンパク質Ysw1の出芽酵母前胞子膜形成における役割2019

    • Author(s)
      棟重賢治 藤枝祐二 須田恭之 舘川宏之
    • Organizer
      酵母遺伝学フォーラム
    • Related Report
      2019 Annual Research Report
  • [Presentation] 出芽酵母減数分裂における膜交通関連オルガネラの再構成2019

    • Author(s)
      須田恭之 舘川宏之 中野明彦 入江賢児
    • Organizer
      酵母遺伝学フォーラム
    • Related Report
      2019 Annual Research Report
  • [Presentation] 出芽酵母胞子壁に結合するRNAの解析2019

    • Author(s)
      Yang Yan Song Chaoqun 舘川宏之 高暁冬 中西秀樹
    • Organizer
      酵母遺伝学フォーラム
    • Related Report
      2019 Annual Research Report
  • [Presentation] メンブレンコンタクトサイトの再配置による脂質輸送と膜伸長の分子機構解明2019

    • Author(s)
      舘川宏之 須田恭之
    • Organizer
      オルガネラ・ゾーン研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 酵母の前胞子膜形成におけるリン脂質とオルガネラ接触部位の役割2019

    • Author(s)
      舘川宏之
    • Organizer
      日本農芸化学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 出芽酵母前胞子膜形成の研究-新しい膜構造を構築する分子メカニズムの理解を目指して-2018

    • Author(s)
      舘川宏之
    • Organizer
      酵母細胞研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] Post-Golgi vesicle tethering and fusion for de novo membrane formation during sporulation in budding yeast2018

    • Author(s)
      Yasuyuki Suda, Hiroyuki Tachikawa, Kenji Irie, Akihiko Nakano
    • Organizer
      FEBS special meeting -the 2018 Golgi meeting-
    • Related Report
      2018 Research-status Report
  • [Presentation] 出芽酵母の前胞子膜伸長における PI4P およびオルガネラ接触部位の役割の解析2018

    • Author(s)
      中村毅  棟重賢治  須田恭之  舘川宏之
    • Organizer
      酵母遺伝学フォーラム
    • Related Report
      2018 Research-status Report
  • [Presentation] 出芽酵母の胞子が貪食細胞および非貪食細胞において取り込まれる過程の解析2018

    • Author(s)
      Wang Qin Yang Yan Liu Guoyu 舘川宏之 高暁冬 中西秀樹
    • Organizer
      酵母細胞研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] Analysis of the role of PI4P and organelle contact site in prospore membrane extension during sporulation of budding yeast2018

    • Author(s)
      Tsuyoshi S Nakamura, Kenji Muneshige, Yasuyuki Suda, Hiroyuki Tachikawa
    • Organizer
      日本細胞生物学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Studies on phagocytic uptake of yeast spores2018

    • Author(s)
      Hideki Nakanishi, Qin Wang, Yang Yan, Xiao-Dong Gao, Hiroyuki Tachikawa
    • Organizer
      日本細胞生物学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Analysis of the role of PI4P in prospore membrane extension during sporulation of budding yeast2017

    • Author(s)
      Nakamura Tsuyoshi S.、Okumura Yuuya、Tachikawa Hiroyuki
    • Organizer
      28th International Conference on Yeast Genetics and Molecular Biology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 出芽酵母前胞子膜伸長におけるPI4Pの役割2017

    • Author(s)
      中村毅、舘川宏之
    • Organizer
      酵母遺伝学フォーラム
    • Related Report
      2017 Research-status Report
  • [Presentation] 出芽酵母の前胞子膜伸長に必要な PP1 ターゲティングサブユニット Gip1 の解析2017

    • Author(s)
      中村毅、奥村祐哉、井上一朗、須田恭之、舘川宏之
    • Organizer
      日本細胞生物学会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2022-02-21  

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