Horizontal gene transfer in biofilm and evolution

Research Project

Project/Area Number	17K07714
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Applied microbiology
Research Institution	National Institute of Advanced Industrial Science and Technology
Principal Investigator	Nakashima Nobutaka 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ長 (70357622)
Project Period (FY)	2017-04-01 – 2020-03-31
Project Status	Completed (Fiscal Year 2019)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords	バイオフィルム / 次世代シーケンサ / 遺伝子水平伝播 / 微生物群集 / 微生物集団 / 細菌
Outline of Final Research Achievements	Biofilm samples were obtained from the bottom of a pond in Tsukuba city, and the samples were divided into two. Only one sample was treated with DNase. Genomic DNA was purified from a total of 6 samples, and the obtained DNA samples were subjected to Hiseq sequencing. The sequencing results were manipulated according to the MetaWRAP pipeline. As a result of assembling and Binning, Bins were obtained as expected. Some Bins were found to have statistically significant abundances with and without DNase treatment. Also, we examined changes in gene composition that did not depend on Binning, and revealed the existence of statistically significantly different species with and without DNase treatment.
Academic Significance and Societal Importance of the Research Achievements	本研究は、微生物の多様性獲得のメカニズム、ゲノム進化の過程を解明するにあたって大変意義深いものである。そればかりではなく、バイオフィルムの有効利用、人体や環境に悪影響を与えるバイオフィルムの効果的除去のための基盤情報となる。なお、バイオフィルム内の微生物は抗生物質や各種化学物質、加熱に対して抵抗性を示し、現状ではその除去が困難である。