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Oxidation of various compounds by quinoproteins with broad substrate specificities

Research Project

Project/Area Number 17K07722
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionYamaguchi University

Principal Investigator

Yakushi Toshiharu  山口大学, 大学院創成科学研究科, 教授 (30324388)

Co-Investigator(Kenkyū-buntansha) 片岡 尚也  山口大学, 大学院創成科学研究科, 助教 (50713509)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords酢酸菌 / 発酵 / 酵素 / 応用微生物 / バイオテクノロジー / 微生物 / ゲノム
Outline of Final Research Achievements

The incomplete oxidation, or referred to as oxidative fermentation, of acetic acid bacteria is a safe microbial use that has been familiar to human since ancient times, typically vinegar making. This study attempted to analyze the incomplete oxidation of acetic acid bacteria, which remains undeveloped, from the viewpoint of utilizing unused biological, enzymatic, and genetic resources. First, we obtained a mutant strain in which the high oxidation ability of the wild-type acetic acid bacteria was reduced. Furthermore, the potential of the target enzyme in this study was analyzed by constructing the mutant strains producing uncharacterized and chacterized enzymes. The results confirmed (i) the activity of two new enzymes, (ii) enhanced weak substance conversion ability using known enzymes, and (iii) novel substrate oxidation of a known enzyme.

Academic Significance and Societal Importance of the Research Achievements

近年,持続可能な開発目標(SDGs)を頻繁に耳にするようになり,世界レベルで持続可能な社会づくりの基盤整備が求められている。例えば,地下資源に頼らない化学製品の開発に微生物を利用するということが試みられている。酢酸菌は,高速に化学反応(酸化)を触媒できるので,この力を利用し,かつ今まで知られていなかった物質酸化反応に挑戦した。本研究からいくつかの成果が得られたが,総じてこのような試みによって,新しい可能性を提示することができたことが最大の成果である。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (32 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (12 results) Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results) Presentation (11 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (2 results)

  • [Int'l Joint Research] ユーリッヒ研究所(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] カセサート大学/ラジャマンガラ工科大学イサン校(タイ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ラオス国立大学(ラオス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] カントー大学(ベトナム)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] カセサート大学/ラジャマンガラ工科大学イサン校(タイ)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] ユーリッヒ研究所(ドイツ)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] ラオス国立大学(ラオス)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] カントー大学(ベトナム)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] カセサート大学/チュラロンコン大学/ラジャマンガラ工科大学イサン校(タイ)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] ユーリッヒ研究所(ドイツ)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] ラオス国立大学(ラオス)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] カントー大学(ベトナム)

    • Related Report
      2017 Research-status Report
  • [Journal Article] The membrane-bound sorbosone dehydrogenase of Gluconacetobacter liquefaciens is a pyrroloquinoline quinone-dependent enzyme2020

    • Author(s)
      Toshiharu Yakushi, Ryota Takahashi, Minenosuke Matsutani, Naoya Kataoka, Roque A. Hours, Yoshitaka Ano, Osao Adachi, Kazunobu Matsushita
    • Journal Title

      Enzyme and Microbial Technology

      Volume: 137 Pages: 109511-109511

    • DOI

      10.1016/j.enzmictec.2020.109511

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Pyrroloquinoline quinone-dependent dehydrogenases of acetic acid bacteria2018

    • Author(s)
      Matsutani M, Yakushi T
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 102 Issue: 22 Pages: 9531-9540

    • DOI

      10.1007/s00253-018-9360-3

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] ゲノム情報を活用するGluconobacter属酢酸菌の分子生物学研究2018

    • Author(s)
      阿野 嘉孝,藥師 寿治
    • Journal Title

      化学と生物

      Volume: 56 Pages: 414-421

    • Related Report
      2018 Research-status Report
  • [Journal Article] Aldopentoses as new substrates for the membrane-bound, pyrroloquinoline quinone-dependent glycerol (polyol) dehydrogenase of Gluconobacter sp.2018

    • Author(s)
      Yakushi T, Terada Y, Ozaki S, Kataoka N, Akakabe Y, Adachi O, Matsutani M, Matsushita K
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 102 Issue: 7 Pages: 3159-3171

    • DOI

      10.1007/s00253-018-8848-1

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Improved heterologous expression of the membrane-bound quinoprotein quinate dehydrogenase from Gluconobacter oxydans2018

    • Author(s)
      Yakushi T, Komatsu K, Matsutani M, Kataoka N, Vangnai AS, Toyama H, Adachi O, Matsushita K
    • Journal Title

      Protein Expr Purif

      Volume: 145 Pages: 100-107

    • DOI

      10.1016/j.pep.2018.01.007

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Role of a membrane-bound aldehyde dehydrogenase complex AldFGH in acetic acid fermentation with Acetobacter pasteurianus SKU11082018

    • Author(s)
      Yakushi T, Fukunari S, Kodama T, Matsutani M, Nina S, Kataoka N, Theeragool G, Matsushita K
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 印刷中 Issue: 10 Pages: 4549-4561

    • DOI

      10.1007/s00253-018-8940-6

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A single-nucleotide insertion in a drug transporter gene induces a thermotolerant phenotype of Gluconobacter frateurii by increasing the NADPH/NADP+ ratio via metabolic change2018

    • Author(s)
      Matsumoto N, Hattori H, Matsutani M, Matayoshi C, Toyama H, Kataoka N, Yakushi T, Matsushita K
    • Journal Title

      Appl Environ Microbiol

      Volume: - Issue: 10

    • DOI

      10.1128/aem.00354-18

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Two tales of the respiratory chain: 呼吸鎖が物質生産に直接的に関わるお話と間接的に関わるお話2019

    • Author(s)
      薬師 寿治,片岡 尚也,松下 一信
    • Organizer
      日本生物工学会大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Gluconobacter属酢酸菌由来5-ケト-フルクトース還元酵素の構造と機能2019

    • Author(s)
      グエン ミン ツイ,後藤 勝,野田 将平,片岡 尚也,足立 収生,松下 一信,薬師 寿治
    • Organizer
      日本生化学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Komagataeibacter属酢酸菌の膜結合型アルコール脱水素酵素の機能解析2018

    • Author(s)
      藥師寿治,向井妃奈,ニタヤ ピティウィッタヤクル,松谷峰之介,ポーンチャノック タウィーチープ,片岡尚也,ガンジャナ ティーラーグール,松下一信
    • Organizer
      日本農芸化学会中四国支部
    • Related Report
      2018 Research-status Report
  • [Presentation] Gluconacetobacter属酢酸菌のピロロキノリンキノン依存型ソルボソン脱水素酵素の解析2018

    • Author(s)
      高橋亮太,松谷峰之介,片岡尚也,藥師寿治,松下一信
    • Organizer
      日本農芸化学会中四国支部
    • Related Report
      2018 Research-status Report
  • [Presentation] New substrates and products on the action of PQQ-dependent glycerol dehydrogenase of Gluconobacter sp.2018

    • Author(s)
      Toshiharu Yakushi, Yuka Terada, Seishiro Ozaki, Naoya Kataoka, Yoshihiko Akakabe, Osao Adachi, Minenosuke Matsutani, and Kazunobu Matsushita
    • Organizer
      5th International Conference on Acetic Acid Bacteria
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Gluconacetobacter属酢酸菌の膜結合型ソルボソン脱水素酵素はピロロキノリンキノン依存酵素である2018

    • Author(s)
      高橋 亮太, 松谷 峰之介, 片岡 尚也, Roque A. Hours, 足立 収生, 松下 一信, 藥師 寿治
    • Organizer
      日本生化学会
    • Related Report
      2018 Research-status Report
  • [Presentation] アセトバクター属酢酸菌の細胞表層ではたらく膜結合型アルデヒド脱水素酵素の機能解析2018

    • Author(s)
      一木 愛,新納 俊,松谷峰之介,片岡尚也,薬師寿治,ガンジャナ ティーラグール,松下一信
    • Organizer
      日本生化学会中国四国支部
    • Related Report
      2018 Research-status Report
  • [Presentation] Gluconobacter属酢酸菌から精製したPQQ依存型グリセロール脱水素酵素を用いたL-リボースの酸化2018

    • Author(s)
      寺田 優花,尾崎 聖士朗,片岡 尚也,足立 収生,赤壁 善彦,藥師 寿治,松下 一信
    • Organizer
      日本農芸化学会中四国支部
    • Related Report
      2017 Research-status Report
  • [Presentation] 酢酸菌の膜結合型アルデヒド脱水素酵素の分子種と発現:酢酸発酵との関連2018

    • Author(s)
      福成 聖也,松谷 峰之介,薬師 寿治,片岡 尚也,ガンジャナ ティーラグール,松下 一信
    • Organizer
      日本農芸化学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Gluconacetobacter 属酢酸菌の膜結合型ソルボソン脱水素酵素の解析2017

    • Author(s)
      高橋 亮太, 松谷 峰之介, 片岡 尚也, 藥師 寿治, 松下 一信
    • Organizer
      日本生物工学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 酢酸菌膜結合型ヘテロ三量体ソルビトール脱水素酵素の分子構築:小サブユニットの役割2017

    • Author(s)
      藥師 寿治,Soemphol Wichai,Phong Xuan Huynh,片岡 尚也,外山 博英,松下 一信
    • Organizer
      日本生化学会
    • Related Report
      2017 Research-status Report
  • [Remarks] 山口大学農学部応用微生物学研究室

    • URL

      http://web.cc.yamaguchi-u.ac.jp/~oubi/

    • Related Report
      2019 Annual Research Report 2018 Research-status Report 2017 Research-status Report
  • [Remarks] 酢酸菌研究会

    • URL

      http://web.cc.yamaguchi-u.ac.jp/~jaab/

    • Related Report
      2019 Annual Research Report 2018 Research-status Report 2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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