| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
L-[35S] cysteine was added as a tracer to a cell-free extract of Clostridium acetobutylicum, and analyzed by autoradiography. NifU-like protein was found to be radiolabeled with 35S. The nifU gene was amplified by PCR and inserted into the pCold I vector to construct an expression system and recombinant NifU-like proteins were obtained. With sulfur transfer system by E. coli cysteine desulfurase IscS and L-[35S]cysteine, the sulfur accepting capacity of the NifU-like protein was analyzed in vitro. The amount of 35S radiolabeled was increased with time, confirming that it has a sulfur receptor capacity.
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