| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The purpose of this research was to elucidate the structure-function relationship of glucoside hydrolase (GH) family enzymes and their applications. Among GH15 enzymes, in addition to known trehalose-hydrolyzing enzymes from euryarchaea Thermoplasma sp., two trehalases from crenarchaeon Sulfolobus could be expressed as soluble active forms and their properties were characterized. In Thermoplasma enzyme, I identified some amino acid residues involving with its catalytic reaction in conserved regions forming its putative active site using protein engineering method, analyzed functions of its putative N-terminal domain, and constructed the basic condition of its crystallization for structural analysis.
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