| Project/Area Number |
17K07731
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Toyo University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 有機溶媒 / 大腸菌 / キノン / 有機溶媒耐性 / 応用微生物 |
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| Outline of Final Research Achievements |
Biotechnological applications for the synthesis of various valuable chemicals can reduce wastes, save raw materials, and conserve energy. Enhancement of organic solvent-tolerance in microorganisms is expected to enhance the production level of valuable chemicals in the presence of organic solvent. AcrAB-TolC efflux pump plays an important role in organic solvent-tolerance in E. coli. Three homologous transcription activators MarA, SoxS, and Rob regulate a common set of genes known as the mar-sox-rob regulons such as acrAB, and tolC involved in organic solvent-tolerance in E. coli. In this study, we found that 1,4-dihydroxy-2-naphthoic acid (DHNA) increases organic solvent-tolerance in E. coli. AcrAB-TolC pump was found to be involved in the improvement of organic solvent tolerance by DHNA. In addition, it was found that homologous transcription factors MarA and SoxS are involved in the expression of AcrAB-TolC efflux pump.
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| Academic Significance and Societal Importance of the Research Achievements |
バイオ燃料や化学製品等の有用物質生産の効率化のため、微生物の有機溶媒耐性機構が注目されている。化学製品の製造プロセスにおいて一般的に使用されている有機溶媒の存在下では、有機溶媒の細胞毒性のため、生産効率が著しく低下する。このため、用いる細胞に有機溶媒耐性を付与することが重要な課題である。本研究の成果はバイオ燃料や化学製品を製造する幅広い産業分野に普及することが期待される。
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