| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Biocatalysts catalyzing domino-type oxidation-asymmetric cyclization or iminium ion asymmetric reduction were developed and were studied for redox-neutral synthetic process of bioactive alkaloids. We have screened for microorganisms that catalyze domino-type oxidation-asymmetric cyclization or iminium ion asymmetric reduction, which are the key processes in this study, using stock cultures and soil microorganisms. Although the desired biotransformations were not achieved with high stereoselectivity, efficient pathways for the synthesis of substrates and standard products starting from commercially available tryptamine or 3,4-dimethoxyphenethylamine were established, and allowed for biotransformations using wet cells and enzyme activity measurements using cell-free extracts.
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